A5011463781- HIV Research and Treatment
- Immune Cell Function and Interaction
- HIV/AIDS Research and Interventions
- HIV/AIDS drug development and treatment
- T-cell and B-cell Immunology
- HIV-related health complications and treatments
- Cytomegalovirus and herpesvirus research
- interferon and immune responses
- Epigenetics and DNA Methylation
- Animal Disease Management and Epidemiology
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Antimicrobial Peptides and Activities
- HIV/AIDS oral health manifestations
- Immunotherapy and Immune Responses
- Herpesvirus Infections and Treatments
- Bioactive Natural Diterpenoids Research
- Systemic Lupus Erythematosus Research
- Viral-associated cancers and disorders
- Respiratory viral infections research
- Parvovirus B19 Infection Studies
- SARS-CoV-2 detection and testing
- Vaccine Coverage and Hesitancy
- Biological Stains and Phytochemicals
Université Paris Cité
2021-2025
Institut Pasteur
2016-2025
Inserm
2017-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2017-2024
Infectious Disease Models and Innovative Therapies
2017-2024
CEA Paris-Saclay
2020-2024
Université Paris-Saclay
2020-2024
Institut thématique Immunologie, inflammation, infectiologie et microbiologie
2021-2022
Université Paris-Sud
2017-2020
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2017-2020
Abstract Despite scientific evidence originating from two patients published to date that CCR5Δ32/Δ32 hematopoietic stem cell transplantation (HSCT) can cure human immunodeficiency virus type 1 (HIV-1), the knowledge of immunological and virological correlates is limited. Here we characterize a case long-term HIV-1 remission 53-year-old male who was carefully monitored for more than 9 years after allogeneic HSCT performed acute myeloid leukemia. sporadic traces DNA detected by droplet...
Abstract HIV remission can be achieved in some people, called post-treatment controllers, after antiretroviral treatment discontinuation. Treatment initiation close to the time of infection was suggested favor control, but circumstances and mechanisms leading this outcome remain unclear. Here we evaluate impact early (week 4) vs. late 24 post-infection) SIVmac 251 -infected male cynomolgus macaques receiving 2 years therapy before analytical interruption. We show that strongly promotes which...
Virus-specific CD8+ T cells play a major role in the natural control of HIV infection, linked to memory-like features such as high survival capacity and polyfunctionality. However, virus-specific from non-controllers exhibit an effector-like exhausted profile, with limited antiviral potential. Metabolic reprogramming could reinvigorate their functional capacities. Considering implication cholesterol pathway induction cell exhaustion, here we evaluated impact rosuvastatin, inhibitor...
The existence of HIV reservoirs in infected individuals under combined antiretroviral therapy (cART) represents a major obstacle toward cure. Viral are assessed by quantification nucleic acids, method which does not discriminate between infectious and defective viruses, or viral outgrowth assays, require large numbers cells long-term cultures. Here, we used an ultrasensitive p24 digital assay, report to be 1,000-fold more sensitive than classical enzyme-linked immunosorbent assays (ELISAs)...
Virus-specific CD8+ T cells play a central role in HIV-1 natural controllers to maintain suppressed viremia the absence of antiretroviral therapy. These display memory program that confers them stemness properties, high survival, polyfunctionality, proliferative capacity, metabolic plasticity, and antiviral potential. The development maintenance such qualities by appear crucial achieving control. Here, we show targeting signaling pathways Wnt/transcription factor cell 1 (Wnt/TCF-1) mTORC...
To investigate the origin and to reconstruct onset date of HIV-1 subtype C epidemic in Brazil.Three independent datasets sequences isolated from HIV-1-positive patients southern Brazil over a period 15 years (1991-2006) were analyzed: 82 env V3 (213 nt), 40 C2-C5 (559 72 pol (960 nt).Brazilian compared with other reference strains database using basic local alignment search tool, phylogenetic analyses, searching specific amino acid signature patterns. Evolutionary parameters estimated...
Elevated blood CXCL10/IP-10 levels during primary HIV-1 infection (PHI) were described as an independent marker of rapid disease onset, more robust than peak viremia or CD4 cell nadir. IP-10 enhances the recruitment CXCR3+ cells, which include major HIV-target raising question if it promotes establishment viral reservoirs. We analyzed data from four cohorts HIV+ patients, allowing us to study before (Amsterdam cohort), well controlled and uncontrolled (ANRS cohorts). also addressed...
Type I IFN (IFN-I) production by plasmacytoid DCs (pDCs) occurs during acute HIV-1 infection in response to TLR7 stimulation, but the role of pDC-derived IFN-I controlling or promoting is ambiguous. We report here a sex-biased interferogenic phenotype for frequent single-nucleotide polymorphism human TLR7, rs179008, displaying an impact on key parameters infection. show allele rs179008 T determine lower protein abundance cells from women, specifically — likely diminishing mRNA translation...
Abstract Natural killer (NK) cells play a critical understudied role during HIV infection in tissues. In natural host of SIV, the African green monkey (AGM), NK mediate strong control SIVagm secondary lymphoid We demonstrate that induces expansion terminally differentiated NKG2a low organs displaying an adaptive transcriptional profile and increased MHC-E-restricted cytotoxicity response to SIV Env peptides while expressing little IFN-γ. Such cell differentiation was lacking SIVmac-infected...
While most individuals suffer progressive disease following HIV infection, a small fraction spontaneously controls the infection. Although CD8 T-cells have been implicated in this natural control, their mechanistic roles are yet to be established. Here, we combined mathematical modeling and analysis of previously published data from 16 SIV-infected macaques, which 12 were controllers, elucidate role control. For each macaque, considered, addition canonical vivo plasma viral load SIV DNA...
The mechanisms behind natural control of HIV replication are still unclear, and several studies pointed that elite controllers (ECs) a heterogeneous group.We performed analyses virologic, genetic, immunologic parameters HIV-1 groups: (1) ECs (viral load, <80 copies/mL); (2) ebbing (EECs; transient viremia/blips); viremic (VCs; detectable viremia, <5000 copies/mL). Untreated noncontrollers (NCs), patients under suppressive highly active antiretroviral therapy (HAART), HIV-1-negative...
SARS-CoV-2 has caused global disruptions, prompting studies on immune responses to COVID-19 vaccines, particularly antibodies against the Spike (S) protein. However, Nucleocapsid (N) protein remain less explored. This study evaluated whether CoronaVac induces anti-N antibodies, and analyzed antibody dynamics after a BNT162b2 booster, given that targets both S N proteins, while only Serum samples were collected at multiple intervals post-vaccination. The percentage of participants with...
The AIDS epidemic in Southern Brazil has unique features, showing co-circulation of HIV-1 subtypes C, B and recombinant forms. Florianópolis the second highest incidence among Brazilian capitals, but limited information is available about HIV molecular epidemiology prevalence primary drug resistance.To investigate to describe resistance mutations (DMRs).Epidemiological clinical data from 82 untreated patients (2008-2009) were analyzed. subtype at envelope, protease, reverse transcriptase...
The multiplicity, heterogeneity, and dynamic nature of human immunodeficiency virus type-1 (HIV-1) latency mechanisms are reflected in the current lack functional cure for HIV-1. Accordingly, all classes latency-reversing agents (LRAs) have been reported to present variable ex vivo potencies. Here, we investigated molecular underlying potency variability one LRA: DNA methylation inhibitor 5-aza-2'-deoxycytidine (5-AzadC).
Background: Antiretroviral drugs targeting integrase (IN) have recently been approved for use in combined and salvage therapeutic interventions. Objective: To evaluate the presence of natural polymorphisms resistance mutations associated with IN inhibitors among HIV-1 subtypes B, C, F samples obtained from drug-naive individuals patients failing highly active antiretroviral therapy Brazil. Methods: Proviral DNA was blood 105 HIV-1-positive infected by or plasma viral RNA 30 subtype...
Heterogenous immune reconstitution and de novo priming of HIV-specific T cells characterize allo-HSCT recovery in HIV-infected individuals.
Highly efficient CD8+ T cells are associated with natural HIV control, but it has remained unclear how these generated and maintained. We have used a macaque model of spontaneous SIVmac251 control to monitor the development cell responses. Our results show that SIV-specific emerge during primary infection in all animals. The ability suppress SIV is suboptimal acute phase increases progressively controller macaques before establishment sustained low-level viremia. Controller develop optimal...
Abstract We previously demonstrated that alcoholic extracts from Pterodon pubescens Benth. (Sucupira branca, Leguminosae) seeds exhibit anti-arthritic activity. In the present work we show oleaginous extract obtained P. (OEP) exhibits acute or topic anti-edematogenic activity when tested in carrageenan-induced paw edema croton oil-induced ear assays, respectively. Four fractions were OEP by sequential liquid–liquid extraction. The properties predominant hexanic fraction, which was further...