A5009164884- Hematopoietic Stem Cell Transplantation
- Acute Myeloid Leukemia Research
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- Acute Lymphoblastic Leukemia research
- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- Chronic Myeloid Leukemia Treatments
- T-cell and B-cell Immunology
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Immunodeficiency and Autoimmune Disorders
- SARS-CoV-2 detection and testing
- Renal Transplantation Outcomes and Treatments
- Viral-associated cancers and disorders
- Transplantation: Methods and Outcomes
- COVID-19 Clinical Research Studies
- Renal and related cancers
- Polyomavirus and related diseases
- Mesenchymal stem cell research
- Cancer-related Molecular Pathways
- Advanced Breast Cancer Therapies
- Respiratory viral infections research
Hospital General Universitario Gregorio Marañón
2016-2025
Universidad Carlos III de Madrid
2024
Universidad Complutense de Madrid
2021
Instituto de Biomedicina de Valencia
2021
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias
2021
Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública
2021
Institute for Integrative Systems Biology
2021
Universitat de València
2021
Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana
2021
John Wiley & Sons (United States)
2018
This article has been corrected. The original version (PDF) is appended to this as a Supplement. Background: multifactorial mechanisms associated with radical reductions in HIV-1 reservoirs after allogeneic hematopoietic stem cell transplant (allo-HSCT), including case of HIV cure, are not fully understood. Objective: To investigate the mechanism eradication allo-HSCT. Design: Nested series within IciStem observational cohort. Setting: Multicenter European study. Participants: 6...
Abstract Chimeric antigen receptor (CAR) T cell–related HLH/MAS is an unusual manifestation of severe cytokine release syndrome (CRS) with poor prognosis and a challenging diagnosis. The establishment specific diagnosis criteria essential, the combination several techniques for CAR T‐cell follow‐up, allows more precise management this complication.
Relapse remains the main cause of treatment failure in patients with acute myelogeous leukemia (AML) after allogeneic hemopoietic stem cell transplantation (SCT). The Wilms' tumor 1 gene (WT1) is reportedly overexpressed >90% AML and thus can be useful for minimal residual disease (MRD) monitoring. aim this study was to evaluate usefulness WT1 expression as a relapse predictor marker SCT compare it flow cytometry (FC) chimerism studies. assessed retrospectively using quantitative RT-PCR bone...
Abstract Allogeneic stem cell transplantation (allo‐ SCT ) has become the treatment of choice in patients with intermediate‐risk and high‐risk acute myeloid leukemia ( AML ). The quality response to treatment, assessed terms detection minimal residual disease MRD ), been consistently associated prognosis clinical outcome . aim present study was evaluate prognostic impact analyzing bone marrow using 4‐color multiparametric flow cytometry MFC 29 before after allo‐ Eighteen who were shown be...
We aimed at assessing the clinical significance of levels acute lymphoblastic leukemia (ALL) cells in samples cerebrospinal fluid (CSF) during therapy. studied 990 CSF from 108 patients, time diagnosis (108) and each intrathecal therapy (882). The proportions leukemic were assessed by flow cytometry (FCM). Patients with central nervous system (CNS) involvement (FCM+) showed predominantly a T-ALL, higher percentages known negative prognostic factors: high risk group, white blood cell counts,...
A successful Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variant, B.1.1.7, has recently been reported in the UK, causing global alarm. Most likely, new variant emerged a persistently infected patient, justifying special focus on these cases. Our aim this study was to explore certain clinical profiles involving severe immunosuppression that may help explain prolonged persistence of viable viruses. We present three severely immunosuppressed cases (A, B, and C) with history...
Chimeric antigen receptor T-cells (CAR-T) has emerged as a promising therapy, over 60% of patients fail to sustain long-term response. The underlying factors that leads the effectiveness this therapy are not completely understood, CAR-T cell persistence and monitoring seems be pivotal for ensuring successful Various methods such multiparametric flow cytometry (MFC) or quantitative PCR (qPCR) have been applied. Our objective is develop digital (dPCR) assays detection quantification cells,...
Posttransplant cyclophosphamide (PTCy) effectively prevents graft-versus-host disease (GVHD) after HLA-haploidentical hematopoietic stem cell transplantation (HSCT). The use of PTCy in HLA-identical HSCT is less explored. We conducted a retrospective study 107 consecutive patients undergoing an sibling (10/10) 2 centers Spain, 50 with GVHD prophylaxis methotrexate-cyclosporin A (MTX-CsA) and 57 using PTCy-based regimen additional immunosuppression. Graft source was unmanipulated mobilized...
Chimerism refers to the relative proportion of donor and recipient DNA after hematopoietic stem cell transplantation (HSCT) its quantitative follow-up is great clinical utility in this setting. PCR short tandem repeats (STR-PCR) constitutes gold standard method for chimerism quantification, although more sensitive techniques (such as qPCR) have recently arisen. We compared sensitivity quantification capacity both patient samples artificial mixtures demonstrated adequate performance methods,...
EZH2 is mutated in nearly 25% of follicular lymphoma (FL) cases. Little known about how affects patients' response to therapy. In this context, the aim study was retrospectively analyze frequency mutations at diagnosis tissue and ctDNA patients with FL assess outcomes after receiving immunochemotherapy, depending on mutation status. Among 154 included study, 27% had (46% high-grade 26% low-grade FL). Of samples, identified 44% not any patient unmutated tissue.Unmutated who received R-CHOP...
Abstract In chronic lymphocytic leukemia, the reliability of next‐generation sequencing (NGS) to detect TP53 variants ≤10% allelic frequency (low‐VAF) is debated. We tested ability 23 such in 41 different laboratories using their NGS method choice. The sensitivity was 85.6%, 94.5%, and 94.8% at 1%, 2%, 3% VAF cut‐off, respectively. While only one false positive (FP) result reported >2% VAF, it more challenging distinguish true <2% from background noise (37 FPs by 9 laboratories)....
Molecular diagnosis of myeloid neoplasms (MN) is based on the detection multiple genetic alterations using various techniques. Next-generation sequencing (NGS) has been proved as a useful method for analyzing many genes simultaneously. In this context, we analyzed diagnostic samples from 121 patients affected by MN and ten relapse subset acute leukemia two enrichment-capture NGS gene panels. Pathogenicity classification variants was enhanced development application custom onco-hematology...
// Gonzalo Blanco 1, 2, 3 , Anna Puiggros 2 Panagiotis Baliakas 4 Anastasia Athanasiadou 5 MªDolores García-Malo 6 Rosa Collado 7 Aliki Xochelli 4, 8 María Rodríguez-Rivera Margarita Ortega 9 Mª José Calasanz 10 Elisa Luño 11 MªTeresa Vargas 12 Javier Grau 13 Carolina Martínez-Laperche 14 Alberto Valiente 15 Cervera 16 Achilles Anagnostopoulos Eva Gimeno 17 Eugènia Abella Evangelia Stalika Jesús Hernández-Rivas 18...
Heterogenous immune reconstitution and de novo priming of HIV-specific T cells characterize allo-HSCT recovery in HIV-infected individuals.
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a curative treatment for patients with hematologic malignances. Haploidentical HSCT (Haplo-HSCT) an alternative option who do not have HLA-matched donor. The use of post-transplantation high dose cyclophosphamide (PT-Cy) commonly employed graft-versus-host disease (GVHD) prophylaxis in haplo-HSCT. Cyclophosphamide (Cy) alkylating agent antineoplastic and immunosuppressive activity, whose bioactivation requires the activity...
Introduction: Although a number of patients with HIV infection and hematological disease have successfully undergone allogeneic hematopoietic stem-cell transplantation (HSCT), short long-term outcomes remain not well known. We report the largest Spanish experience treating HIV-infected adult high-risk malignancies HSCT. Methods: retrospectively reviewed 22 HIV-positive who received HSCT in five centers Spain. Results: A total were transplanted between 1999 2018. Median age was 44 years. With...
Centers for Disease Control and Prevention guidelines consider SARS-CoV-2 reinfection when sequential COVID-19 episodes occur >90 days apart. However, genomic diversity acquired over recent waves could mean previous infection provides insufficient cross-protection. We used analysis to assess the percentage of early reinfections in a sample 26 patients with 2 separated by 20-45 days. Among sampled patients, 11 (42%) had involving different variants or subvariants. Another 4 cases were...
Background CART therapy has produced a paradigm shift in the treatment of relapsing FL patients. Strategies to optimize disease surveillance after these therapies are increasingly necessary. This study explores potential value ctDNA monitoring with an innovative signature personalized trackable mutations. Method Eleven patients treated anti-CD19 CAR T-cell were included. One did not respond and was excluded. Genomic profiling performed before starting lymphodepleting chemotherapy identify...
Abstract Background SARS-CoV-2 recombinants involving the divergent Delta and Omicron lineages have been described, one of them, “Kraken” (XBB.1.5), has recently a matter concern. Recombination requires coexistence two strains in same individual. Only limited number studies focused on identification co-infections are restricted to Delta/Omicron lineages. Methods We performed systematic throughout pandemic (7609 different patients sequenced), not biassed towards involvement highly Through...