A5027029856- T-cell and B-cell Immunology
- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- Immunotherapy and Immune Responses
- Blood groups and transfusion
- Cytomegalovirus and herpesvirus research
- Acute Myeloid Leukemia Research
- Organ Donation and Transplantation
- Immunodeficiency and Autoimmune Disorders
- Biomedical Ethics and Regulation
- Organ Transplantation Techniques and Outcomes
- vaccines and immunoinformatics approaches
- Transplantation: Methods and Outcomes
- Reproductive System and Pregnancy
- Hemoglobinopathies and Related Disorders
- Prenatal Screening and Diagnostics
- Glycosylation and Glycoproteins Research
- Olfactory and Sensory Function Studies
- Diabetes and associated disorders
- Acute Lymphoblastic Leukemia research
- Single-cell and spatial transcriptomics
- Mesenchymal stem cell research
- Evolutionary Psychology and Human Behavior
- COVID-19 Clinical Research Studies
German National Bone Marrow Donor Registry
2014-2025
Deutsche Knochenmarkspenderdatei
2016-2025
Centre for Genomic Regulation
2024
University of Massachusetts Chan Medical School
2020
WHU – Otto Beisheim School of Management
2014
Banc de Sang i Teixits
2010
École Supérieure des Sciences Économiques et Commerciales
2010
CY Cergy Paris Université
2010
Newcastle University
2010
Fondation de France
2010
Abstract Background A close match of the HLA alleles between donor and recipient is an important prerequisite for successful unrelated hematopoietic stem cell transplantation. To increase chances finding donor, registries recruit many hundred thousands volunteers each year. Many with limited resources have had to find a trade-off cost resolution extent typing newly recruited donors in past. Therefore, we taken advantage recent improvements NGS develop workflow low-cost, high-resolution...
Whether high-dose cytarabine-based salvage chemotherapy, administered to induce complete remission in patients with poor responsive or relapsed acute myeloid leukaemia scheduled for allogeneic haematopoietic stem-cell transplantation (HSCT) after intensive conditioning confers a survival advantage, is unclear. To test chemotherapy before HSCT, aged between 18 and 75 years non-favourable-risk not first induction untreated relapse were randomly assigned 1:1 cytarabine (3 g/m2 intravenously, 1...
A catalog of common, intermediate and well‐documented (CIWD) HLA‐A, ‐B, ‐C, ‐DRB1, ‐DRB3, ‐DRB4, ‐DRB5, ‐DQB1 ‐DPB1 alleles has been compiled from over 8 million individuals using data 20 unrelated hematopoietic stem cell volunteer donor registries. Individuals are divided into seven geographic/ancestral/ethnic groups summarized for each group the total population. P (two‐field) G assignments one four frequency categories: common (≥1 in 10 000), 100 (≥5 occurrences) or not‐CIWD. Overall 26%...
At the DKMS Life Science Lab, Next Generation Sequencing (NGS) has been used for ultra-high-volume high-resolution genotyping of HLA loci last three and a half years. Here, we report on our experiences in HLA, CCR5, ABO, RHD KIR genes using direct amplicon sequencing approach Illumina MiSeq HiSeq 2500 instruments.Between January 2013 June 2016, 2,714,110 samples largely from German, Polish UK-based potential stem cell donors have processed. 98.9% all alleles targeted (HLA-A, -B, -C, -DRB1,...
Homozygous carriers of CCR5-Δ32, a gene variant CC-type chemokine receptor 5 (CCR5), are highly resistant to infections with human immunodeficiency virus type 1 (HIV-1) and therefore preferred stem cell donors for HIV-infected patients. We analyzed CCR5 typing data 1,333,035 potential hematopoietic enlisted three national DKMS donor centers. Allele genotype frequencies were determined 87 countries origin as self-assessed by the donors. CCR5-Δ32 allele ranged from 16.4% in Norwegian sample 0...
Background A catalogue of common and well‐documented (CWD) human leukocyte antigen (HLA), previously established by the American Society for Histocompatibility Immunogenetics (ASHI), is widely used as indicator typing ambiguities to be resolved in tissue transplantation or checking universality any HLA allele world. However, European population samples, which are characterized a substantial level genetic variation, underrepresented ASHI catalogue. Therefore, Population Genetics Working Group...
Leprosy, a chronic infectious disease caused by Mycobacterium leprae (M. leprae), was very common in Europe till the 16th century. Here, we perform an ancient DNA study on medieval skeletons from Denmark that show lesions specific for lepromatous leprosy (LL). First, test remains M. to confirm infection status of individuals and assess bacterial diversity. We assemble 10 complete genomes all differ each other. Second, evaluate whether human leukocyte antigen allele DRB1*15:01, strong LL...
The killer-cell immunoglobulin-like receptor (KIR) genes regulate natural killer cell activity, influencing predisposition to immune mediated disease, and affecting hematopoietic stem transplantation outcome. Owing the complexity of KIR locus, with extensive gene copy number variation allelic diversity, high-resolution characterization has so far been applied only relatively small cohorts. Here, we present a comprehensive high-throughput genotyping approach based on next generation...
The high‐throughput department of DKMS Life Science Lab encounters novel human leukocyte antigen ( HLA ) alleles on a daily basis. To characterise these alleles, we have developed system to sequence the whole gene from 5′‐ 3′‐ UTR for loci A, B, C, DQB1 and DPB1 submission European Molecular Biology Laboratory – Nucleotide Archive EMBL‐ENA IPD‐IMGT / Database. Our workflow is based dual redundant sequencing strategy. Using shotgun an Illumina MiSeq instrument single molecule real‐time SMRT...
Optimizing natural killer (NK) cell alloreactivity could further improve outcome after allogeneic hematopoietic transplantation (alloHCT). The donor’s Killer-cell Immunoglobulin-like Receptor (KIR) genotype may provide important information in this regard. In the past decade, different models have been proposed aiming at maximizing NK activation by activating KIR-ligand interactions or minimizing inhibitory interactions. Alternative classifications intended predicting alloHCT donor...
The characterization of the ABO blood group status is vital for transfusion and solid organ transplantation. Several methods molecular gene, which encodes alleles that give rise to different groups, have been described. However, application those has so far restricted selected samples not applied population-scale analysis. We describe a cost-effective method high-throughput genotyping system by next generation sequencing. Sample specific barcodes sequencing adaptors are introduced during...
The risk of acute graft-versus-host disease (GvHD) after hematopoietic stem cell transplantation is increased with donor-recipient HLA-DPB1 allele mismatching. single-nucleotide polymorphism (SNP) rs9277534 within the 3' untranslated region (UTR) correlates allotype expression and serves as a marker for permissive mismatches. Since not routinely typed, we analyzed 32,681 samples mostly European ancestry to investigate if can be reliably imputed from standard DPB1 genotyping. We confirmed...
Mothers are able to identify the body odour (BO) of their own child and prefer this smell above other BOs. It has hence been assumed that infantile BO functions as a chemosignal promoting targeted parental care. We tested hypothesis examined whether children's BOs signal genetic similarity developmental status mothers. In addition, we assessed facilitate inbreeding avoidance (Westermarck effect). cross-sectional design, N = 164 mothers participated with biological children ( 226 children,...
Pathogens and associated outbreaks of infectious disease exert selective pressure on human populations, any changes in allele frequencies that result may be especially evident for genes involved immunity. In this regard, the 1346-1353 Yersinia pestis-caused Black Death pandemic, with continued plague spanning several hundred years, is one most devastating recorded history. To investigate potential impact Y. pestis immunity genes, we extracted DNA from 36 victims buried a mass grave...
This study explores pregnant women's awareness of cord blood stem cells and their attitude regarding banking options in France, Germany, Italy, Spain, the UK.Questionnaires were distributed six maternities. anonymous self-completed questionnaire included 29 multiple-choice questions based on: 1) sociodemographic factors, 2) access to information about banking, 3) option preferences, 4) donating units (CBUs) research.A total 79% women had little (n = 1620). A 58% heard therapeutic benefits...
Highlights•8/8 and 10/10 donor match rates vary by patient race ethnicity.•Three-year unrelated registry growth resulted in 2% to 4% increase 8/8 rate.•Most searches did not have a matched identified before clarifying typing.•Most patients can find after testing 1to 2 donors, when 1 exists.AbstractThe National Marrow Donor Program's Be The Match Registry® facilitates the worldwide utilization of (URD) grafts for need hematopoietic cell transplantation. In this study, we estimate URD rate...