A5090713091- HIV Research and Treatment
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- HIV/AIDS drug development and treatment
- Immunotherapy and Immune Responses
- HIV-related health complications and treatments
- HIV/AIDS Research and Interventions
- Sex work and related issues
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer Immunotherapy and Biomarkers
- Platelet Disorders and Treatments
- Inflammasome and immune disorders
- Xenotransplantation and immune response
- Signaling Pathways in Disease
- RNA Interference and Gene Delivery
- COVID-19 Impact on Reproduction
- Organ and Tissue Transplantation Research
- Reproductive System and Pregnancy
- Immunodeficiency and Autoimmune Disorders
- Galectins and Cancer Biology
- Cell Adhesion Molecules Research
- Helicobacter pylori-related gastroenterology studies
- Hepatitis C virus research
- Virus-based gene therapy research
Institut Pasteur
2014-2020
Ragon Institute of MGH, MIT and Harvard
2009-2015
Nantes Université
2008-2012
Inserm
2008-2012
Institut de Transplantation Urologie en Nephrologie
2008-2010
Centre Hospitalier Universitaire de Nantes
2008
Despite accumulating evidence for the importance of allospecific CD8(+) regulatory T cells (Tregs) in tolerant rodents and free immunosuppression transplant recipients, mechanisms underlying Treg-mediated tolerance remain unclear. By using a model transplantation mediated by Tregs following CD40Ig treatment rats, this study, we show that accumulation tolerogenic plasmacytoid dendritic (pDCs) allograft spleen but not lymph nodes was associated with induction vascularized recipients. pDCs...
The existence of HIV reservoirs in infected individuals under combined antiretroviral therapy (cART) represents a major obstacle toward cure. Viral are assessed by quantification nucleic acids, method which does not discriminate between infectious and defective viruses, or viral outgrowth assays, require large numbers cells long-term cultures. Here, we used an ultrasensitive p24 digital assay, report to be 1,000-fold more sensitive than classical enzyme-linked immunosorbent assays (ELISAs)...
Regulatory T cells (Tregs) are potent immune modulators, but their role in human immunodeficiency virus type 1 (HIV-1) pathogenesis remains poorly understood. We performed a detailed analysis of the frequency and function Tregs large cohort HIV-1–infected individuals HIV-1 negative controls. While HIV "elite controllers" uninfected had similar Treg numbers frequencies, absolute declined blood gut-associated lymphoid tissue patients with chronic progressive infection. Despite quantitative...
Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design testing microfluidic intracellular systems that cause temporary membrane disruption by rapid mechanical deformation human mouse cells. Dextran, antibody siRNA performance measured in multiple cell types approach's potential to engineer function demonstrated HIV infection studies.
Elevated blood CXCL10/IP-10 levels during primary HIV-1 infection (PHI) were described as an independent marker of rapid disease onset, more robust than peak viremia or CD4 cell nadir. IP-10 enhances the recruitment CXCR3+ cells, which include major HIV-target raising question if it promotes establishment viral reservoirs. We analyzed data from four cohorts HIV+ patients, allowing us to study before (Amsterdam cohort), well controlled and uncontrolled (ANRS cohorts). also addressed...
T cells chronically stimulated with the same peptide tend to express exhaustion markers such as PD-1 or LAG-3. Deficiencies in and LAG-3 pathways have been linked development of autoimmune diseases. IMP761 is a LAG-3-specific humanized agonist Ab immunosuppressive properties both vitro vivo an Ag-specific delayed-type hypersensitivity (DTH) model cynomolgus macaque (Macaca fascicularis). inhibits TCR-mediated NFAT activation Ag-induced human cell proliferation activation. In DTH model,...
T cell dysfunction in the presence of ongoing antigen exposure is a cardinal feature chronic viral infections with persistent high viremia, including HIV-1. Although interleukin-10 (IL-10) has been implicated as an important mediator this dysfunction, regulation IL-10 production HIV-1 infection remains poorly understood. We demonstrated that elevated plasma individuals and blockade signaling results restoration HIV-1-specific CD4 proliferation, gamma interferon (IFN-γ) secretion, and, to...
The impact of CD4+ regulatory T cells (Tregs) on human immunodeficiency virus type 1 (HIV-1) pathogenesis remains incompletely understood. Although it has been shown that Tregs can be infected with HIV-1, the consequences infection a per-cell basis are still unknown. In vitro HIV-GFP and noninfected were isolated by flow-based cell-sorting to investigate Treg suppressive capacity gene expression profiles. Our data show HIV-1-infected significantly less than demonstrated down-regulation genes...
Heterogenous immune reconstitution and de novo priming of HIV-specific T cells characterize allo-HSCT recovery in HIV-infected individuals.
Abstract Compared with HIV-1, HIV-2 infection is characterized by a larger proportion of slow or nonprogressors. A better understanding pathogenesis should open new therapeutic avenues to establish control HIV-1 replication in infected patients. In this study, we studied the production CD8+ T cells and their capacity for viral controllers from French ANRS CO5 cohort. display robust support long-term renewal cell compartment preserving immune resources, including hematopoietic progenitors...
Regulatory T cells (Tregs) are potent immune modulators, but their precise role in HIV pathogenesis remains incompletely understood. Most studies to date have focused on frequencies or phenotypes of 'bulk' Treg populations. However, although antigen-specific Tregs been reported other diseases, HIV-1 epitope-specific not described date. We here report the first identification functional HIV-1-Gag-specific regulatory using human leukocyte antigen class II tetramer staining HIV-1-infected individuals.
While modulation of regulatory T cell (Treg) function and adoptive Treg transfer are being explored as therapeutic modalities in the context autoimmune diseases, transplantation cancer, their role HIV-1 pathogenesis remains less well defined. Controversy persists regarding beneficial or detrimental effects disease, which warrants further detailed exploration. Our objectives were to investigate if functional CD4+ Tregs can be isolated expanded from HIV-1-infected individuals for experimental...
CD4+ Regulatory T cells (Tregs) are potent immune modulators and serve an important function in human homeostasis. Depletion of Tregs has led to measurable increases antigen-specific cell responses vaccine settings for cancer infectious pathogens. However, their role HIV-1 immuno-pathogenesis remains controversial, as they could either suppress deleterious HIV-1-associated activation thus slow disease progression or alternatively HIV-1-specific immunity thereby promote virus spread....
Heme oxygenase-1 (HO-1) is an inducible stress response protein with potent anti-inflammatory activity and recent data suggest a potentially beneficial role in HIV pathogenesis. We investigated the impact of HO-1 novel subset HO-1-specific CD8 regulatory T cells on virus-specific T-cell immunity HIV-1-infected individuals.HO-1 levels were quantified plasma from individuals at different stages HIV-1 disease longitudinally following primary infection. by flow cytometry using human leukocyte...
CD4+ Regulatory T cells (Tregs) are potent immune modulators and serve an important function in human homeostasis. Depletion of Tregs has led to measurable increases antigen-specific cell responses vaccine settings for cancer infectious pathogens. However, their role HIV-1 immuno-pathogenesis remains controversial, as they could either suppress deleterious HIV-1-associated activation thus slow disease progression or alternatively HIV-1-specific immunity thereby promote virus spread....