A5022859817- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Synthesis and Biological Evaluation
- Insect symbiosis and bacterial influences
- Biological Stains and Phytochemicals
- Parasites and Host Interactions
- Mosquito-borne diseases and control
- Environmental Toxicology and Ecotoxicology
- Insect Pest Control Strategies
- Education and Public Policy
- Biochemical and Molecular Research
- Insect-Plant Interactions and Control
- Biotechnology and Related Fields
- Physiological and biochemical adaptations
- Calcium signaling and nucleotide metabolism
- Malaria Research and Control
- Studies on Chitinases and Chitosanases
- Adenosine and Purinergic Signaling
- Pesticide Exposure and Toxicity
- Enzyme Production and Characterization
- Natural Products and Biological Research
- Edible Oils Quality and Analysis
- Aquatic Ecosystems and Phytoplankton Dynamics
- Business and Management Studies
- Invertebrate Immune Response Mechanisms
Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular
2011-2024
Universidade do Estado do Rio de Janeiro
2013-2024
Universidade Federal do Rio de Janeiro
1990-2011
Trypanosoma cruzi proliferate and differentiate inside different compartments of triatomines gut that is the first environment encountered by T. cruzi. Due to its complex life cycle, parasite constantly exposed reactive oxygen species (ROS). We tested influence pro-oxidant molecules H2O2 superoxide generator, Paraquat, as well as, metabolism products vector, with distinct redox status, in proliferation metacyclogenesis. These are heme, hemozoin urate. also antioxidants NAC GSH. Heme induced...
Abstract Background Sepsis is a prevalent condition in critically ill patients and may be associated with thiamine deficiency (TD). The aim of this study was to evaluate the effect TD on inflammation, oxidative stress cellular recruitment sepsis model. Methods experimental model, cecal ligation puncture (CLP), utilized mice comparison sham procedure. following four groups were compared against each other: SHAM AIN93G complete chow, deficient (TD) CLP chow. Thiamine pyrophosphate (TPP) blood...
Abstract Here we investigated H 2 O production and detoxification in the hematophagous hemiptera, Rhodnius prolixus . Superoxide dismutase (SOD) catalyzes dismutation of superoxide radical (O – ). This reaction produces hydrogen peroxide, which is scavenged by antioxidant enzymes such as catalase (CAT). SOD CAT activities were found all tissues studied, being highest midgut. was dose‐dependently inhibited vivo injections 3‐amino‐1,2,4‐triazole (AT). Insects treated with AT showed a twofold...
Heme is a ubiquitous molecule that has number of physiological roles. The toxic effects this have been demonstrated in various models, based on both its pro-oxidant nature and through detergent mechanism. It estimated about 10 mM heme released during blood digestion the blood-sucking bug's midgut. parasite Trypanosoma cruzi, agent Chagas' disease, proliferates midgut insect vector; however, metabolism trypanosomatids remains to be elucidated. Here we provide mechanistic explanation for...
Abstract We previously demonstrated that alcoholic extracts from Pterodon pubescens Benth. (Sucupira branca, Leguminosae) seeds exhibit anti-arthritic activity. In the present work we show oleaginous extract obtained P. (OEP) exhibits acute or topic anti-edematogenic activity when tested in carrageenan-induced paw edema croton oil-induced ear assays, respectively. Four fractions were OEP by sequential liquid–liquid extraction. The properties predominant hexanic fraction, which was further...
Trypanosoma cruzi, the protozoan responsible for Chagas disease, has a complex life cycle comprehending two distinct hosts and series of morphological functional transformations. Hemoglobin degradation inside insect vector releases high amounts heme, this molecule is known to exert number physiological functions. Moreover, absence its complete biosynthetic pathway in T. cruzi indicates heme as an essential trypanosomatid survival. Within hosts, cope with sudden environmental changes...
Abstract Background Plant derived compounds have been shown to be important sources of several anti-cancer agents. As cell cycle deregulation and tumor growth are intimately linked, the discovery new substances targeting events in this biochemical pathway would great value. The anti-leukemic effect an ethanolic extract Pterodon pubescens seeds (EEPp) has previously demonstrated now we show that a terpenic subfraction (SF5) EEPp containing farnesol, geranylgeraniol vouacapan derivatives...
Trypanosoma cruzi, the etiologic agent of Chagas disease, is transmitted through triatomine vectors during their blood-meal on vertebrate hosts. These hematophagous insects usually ingest approximately 10 mM heme bound to hemoglobin in a single meal. Blood forms parasite are transformed into epimastigotes crop which initiates few hours after ingestion. In previous work, we investigated role cell proliferation and showed that addition significantly increased dose-dependent manner [1]. To...
Heme crystallization as hemozoin represents the dominant mechanism of heme disposal in blood feeding triatomine insect vectors Chagas disease. The absence drugs or vaccine for disease causative agent, parasite Trypanosoma cruzi, makes control vector population best available strategy to limit spread. Although and redox homeostasis regulation is critical both insects T. physiological relevance these organisms remains unknown. Here, we demonstrate that selective blockage vivo by antimalarial...
Trypanosoma cruzi, the causative agent of Chagas disease, faces changes in redox status and nutritional availability during its life cycle. However, influence oxygen fluctuation upon biology T. cruzi is unclear. The present work investigated response epimastigotes to hypoxia. parasites showed an adaptation hypoxic condition, presenting increase proliferation a reduction metacyclogenesis. Additionally, cultured hypoxia produced more reactive species (ROS) compared normoxia. analyses...
Geranylgeraniol is a natural isoprenoid with anti-inflammatory properties extracted from the Pterodon pubescens Benth. fruit oil (PpO). In this work, antiplatelet effect of both PpO and geranylgeraniol investigated. ADP-, thrombin- arachidonic acid (AA)-induced aggregation in human rabbit platelets showed prime involvement cascade. The lack any significant inhibition platelet induced by U-46 619 thrombin, associated suppression prostaglandin E2 thromboxane A2 formation demonstrate, for first...
Chagas disease, also known as American trypanosomiasis, is a potentially life-threatening illness caused by the protozoan parasite, Trypanosoma cruzi, and transmitted triatomine insects during its blood meal. Proliferative epimastigotes forms thrive inside in presence of heme (iron protoporphyrin IX), an abundant product digestion, however little about metabolic outcome this signaling molecule parasite. Trypanosomatids exhibit unusual gene transcription employing polycistronic mechanism...