A5080334202- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Synthesis and Biological Evaluation
- Biochemical and Molecular Research
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- Bioactive Compounds and Antitumor Agents
- Influenza Virus Research Studies
- Toxin Mechanisms and Immunotoxins
- Medical and Biological Ozone Research
- Vibrio bacteria research studies
- Monoclonal and Polyclonal Antibodies Research
- Heme Oxygenase-1 and Carbon Monoxide
- Mollusks and Parasites Studies
- Mosquito-borne diseases and control
- Listeria monocytogenes in Food Safety
- Escherichia coli research studies
- Neonatal Health and Biochemistry
- Diphtheria, Corynebacterium, and Tetanus
- COVID-19 Clinical Research Studies
- Carbohydrate Chemistry and Synthesis
- Biochemical and Structural Characterization
- Insect and Pesticide Research
- Helminth infection and control
- Infection Control and Ventilation
Fundação Oswaldo Cruz
2018-2025
International Olive Council
2024
Universidade Federal Fluminense
2012-2022
Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais
2022
(1) Background: Disinfection of medical devices designed for clinical use associated or not with the growing area tissue engineering is an urgent need. However, traditional disinfection methods are always suitable some biomaterials, especially those sensitive to chemical, thermal, radiation. Therefore, objective this study was evaluate minimal concentration ozone gas (O3) necessary control and kill a set multi-resistant Gram-positive Gram-negative bacteria. The cell viability, membrane...
(1) Background: coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome 2 (SARS-CoV-2), has been linked to hematological dysfunctions, but there are little experimental data that explain this. Spike (S) and Nucleoprotein (N) proteins have putatively associated with these dysfunctions. In this work, we analyzed the recruitment of hemoglobin (Hb) other metabolites (hemin protoporphyrin IX-PpIX) SARS-Cov2 using different approaches. (2) Methods: shotgun proteomics...
Background: The diphtheria toxoid antigen is a major component in pediatric and booster combination vaccines known to raise protective humoral immune response upon vaccination. Although antibodies are considered critical for protection, little about the antigenic determinants that maintain immunity. Methods: One-hundred twelve 15 mer peptides covering entire sequence of toxin (DTx) protein were prepared by SPOT synthesis. immunoreactivity membrane-bound with sera from mice immunized triple...
Health care-associated infections (HAIs) are a significant public health problem worldwide, favoring multidrug-resistant (MDR) microorganisms. The SARS-CoV-2 infection was negatively associated with the increase in antimicrobial resistance, and ESKAPE group had most impact on HAIs. study evaluated bactericidal effect of high concentration O3 gas some reference bacteria.Four standard strains four clinical or environmental MDR were exposed to elevated ozone doses at different concentrations...
Acinetobacter baumannii is a prevalent pathogen in hospital settings with increasing importance infections associated biofilm production. Due to rapid increase its drug resistance and the failure of commonly available antibiotics treat A. infections, this bacterium has become critical public health issue. For these multi-drug resistant baumannii, polymyxin are considered only option for treatment severe infections. Concerning, several polymyxin-resistant strains have been isolated over last...
Abstract: The worldwide spread of SARS-CoV-2 has led to a significant economic and social burden on global scale. Even though the pandemic concluded, there remains apprehension regarding emergence highly transmissible variants capable evading immunity induced by either vaccination or prior infection. success viral penetration is specific amino acid residues Receptor Binding Motif (RBM) involved in attachment. This region interacts with cellular receptor ACE2, triggering neutralizing antibody...
Serological tests have an important and irreplaceable role in the diagnosis of infectious diseases, both for individual patients society. They indirectly identify causative pathogen through capture antibodies, which contrasts with direct detection by antigen molecular biology that are also limited to active infections. Within point-of-care platforms, serodiagnostic assays can 1) support immediate decisions patient care (2) contain spread disease as they do not require highly trained...
The first immune response controls many bacterial and viral inflammatory diseases. Oral immunization with cholera toxin (CT) elicits antibodies can prevent cholerae in endemic environments. While the IgG to is well-documented, IgA IgM epitopes responsible for initial reaction remained uncharted. In this study, our objective was identify characterize immunologically structurally these epitopes. We conducted SPOT synthesis create two libraries, each containing one hundred twenty-two 15-mer...
Background: Chagas disease (CD), a life-threatening caused by Trypanosoma cruzi, remains significant global public health concern. The limited efficacy of the available drugs (nifurtimox and benznidazole), their severe adverse events, unsatisfactory outcomes clinical trials drive search for new, effective, safe drugs. Objective: This study describes synthesis, structural characterization, in vitro antiparasitic activity novel pyrazole-benzimidazole derivatives against mammalian developmental...
Leprosy is a chronic granulomatous disease caused by Mycobacterium leprae affecting the skin and peripheral nerves. Despite M. invasion of keratinocytes importance in innate immunity, interaction these cells vitro during infection poorly understood. Conventional fluorescence optical microscopy, transmission electronic flow cytometry ELISA were used to study with HaCaT human keratinocyte cell line. Keratinocytes uptake described, modulation surface expression CD80 CD209, cathelicidin TNF-α...
Current treatment of cutaneous leishmaniasis includes pentavalent antimonials as first-line drugs, but this therapy has shown severe adverse effects. An alternative to minimize issue is based on combination scheme with other drugs. In study we analyzed the potential association meglumine antimoniate (MA) oxiranes epoxy-α-lapachone (LAP) or epoxymethyl-lawsone (LAW). Results demonstrated that between these drugs enhanced leishmanicidal activity Leishmania (Leishmania) amazonensis infection....
Intracellular parasites such as Trypanosoma cruzi need to acquire valuable carbon sources from the host cell replicate. Here, we investigated energetic metabolism of T. during metacyclogenesis through determination enzymatic activities and quantification by HPLC glycolytic Krebs cycle short-chain carboxylic acids. Altered concentrations in pyruvate, acetate, succinate, glycerate were measured growth epimastigote complex medium BHI their differentiation trypomastigotes chemically defined...
Abstract The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome virus 2 (SARS-CoV-2), has led to a global crisis that included collapsing healthcare systems and shut-down communities, producing considerable economic burden. Despite the number of effective vaccines quickly implemented, emergence new variants is primary concern. scientific community undertook rapid response better study this virus. However, critical questions about viral protein-protein...
(1) Background: Disinfection of medical devices designed for clinical use associated or not with the growing area tissue engineering is an urgent need. However, traditional disinfection methods are always suitable some biomaterials, especially those sensitive to chemical, thermal, radiation. Therefore, objective this study was evaluate minimal concentration ozone gas (O3) necessary control and kill a set multi-resistant Gram-positive Gram-negative bacteria. The cell viability, membrane...
(1) Background: The diphtheria toxoid antigen is a major component in pediatric and booster combination vaccines known to raise protective humoral immune response upon vaccination. Although antibodies are considered critical for protection, little about the antigenic determinants that maintain immunity. (2) Methods: One hundred twelve 15-mer peptides covering entire sequence of DTx protein were prepared by Spot-synthesis. Membrane-bound immunoreactivity with sera from mice immunized triple...
Chagas disease therapy still relies on two nitroderivatives, nifurtimox and benznidazole (Bz), which have important limitations serious adverse effects. New therapeutic alternatives for this silent disease, has become a worldwide public health problem, are essential its control elimination. In study, 1,2,3-triazole analogues were evaluated efficacy against T. cruzi. Three triazole derivatives, 1d (0.21 µM), 1f (1.23 1g (2.28 showed potent activity trypomastigotes, reaching IC50 values 10 to...
The worldwide spread of SARS-CoV-2 has led to a significant economic and social burden on global scale. Even though the pandemic concluded, apprehension remains regarding emergence highly transmissible variants capable evading immunity induced by either vaccination or prior infection. success viral penetration is due specific amino acid residues receptor-binding motif (RBM) involved in attachment. This region interacts with cellular receptor ACE2, triggering neutralizing antibody (nAb)...
Despite successful vaccination efforts, the emergence of new SARS-CoV-2 variants poses ongoing challenges to control COVID-19. Understanding humoral responses regarding infections and their impact is crucial for developing future vaccines that are effective worldwide. Here, we identified 41 immunodominant linear B-cell epitopes in its spike glycoprotein with an SPOT synthesis peptide array probed a pool serum from hospitalized COVID-19 patients. The bioinformatics showed restricted set...
We developed a protein to rapidly and accurately diagnose Chagas disease, life-threatening illness identified by the WHO as critical worldwide public health risk. Complicating current situation are serological test limitations that contribute most infected persons being unaware of their condition therefore untreated. To improve diagnostic testing, we an immunological mimic etiological agent, Trypanosoma cruzi, combining ten pathogen specific epitopes within beta-barrel structure Thermal...