A5077117537- Neonatal Respiratory Health Research
- Preterm Birth and Chorioamnionitis
- Congenital Diaphragmatic Hernia Studies
- Infant Nutrition and Health
- Neonatal and fetal brain pathology
- Neuroscience of respiration and sleep
- Respiratory Support and Mechanisms
- Neonatal and Maternal Infections
- Reproductive System and Pregnancy
- Infant Development and Preterm Care
- Immune Response and Inflammation
- Pregnancy and preeclampsia studies
- Mesenchymal stem cell research
- Anesthesia and Neurotoxicity Research
- Reproductive tract infections research
- Congenital Anomalies and Fetal Surgery
- Neuroinflammation and Neurodegeneration Mechanisms
- TGF-β signaling in diseases
- Pregnancy-related medical research
- Mechanical Circulatory Support Devices
- Anesthesia and Pain Management
- Neurogenesis and neuroplasticity mechanisms
- Infant Health and Development
- Injury Epidemiology and Prevention
- Cardiac Arrest and Resuscitation
Maastricht University
2014-2025
Poznan University of Medical Sciences
2024-2025
The University of Western Australia
2002-2023
King Edward Memorial Hospital
2002-2023
St John of God Subiaco Hospital
2022-2023
Maastricht University Medical Centre
2013-2022
University Medical Center
2009-2022
European Graduate School of Neuroscience
2020-2022
London Women's Clinic
2019
University College London
2019
Extracellular vesicles (EVs), such as exosomes and microvesicles, are released by different cell types participate in physiological pathophysiological processes. EVs mediate intercellular communication cell‐derived extracellular signalling organelles that transmit specific information from their of origin to target cells. As a result these properties, defined may serve novel tools for various therapeutic approaches, including (a) anti‐tumour therapy, (b) pathogen vaccination, (c)...
Abstract Preterm neonates are susceptible to perinatal hypoxic-ischemic brain injury, for which no treatment is available. In a preclinical animal model of injury in ovine fetuses, we have demonstrated the neuroprotective potential systemically administered mesenchymal stromal cells (MSCs). The mechanism MSC unclear but suggested be paracrine, through secretion extracellular vesicles (EVs). Therefore, investigated this study protective effects cell-derived (MSC-EVs) preterm injury. Ovine...
Rationale: Premature infants are exposed to potentially injurious ventilation in the delivery room. Assessments of lung injury confounded by effects subsequent ventilatory support.Objectives: To evaluate response a brief period large tidal volume (Vt) ventilation, simulating neonatal resuscitation preterm neonates.Methods: Preterm lambs (129 d gestation; term is150 d) were ventilated (Vt = 15 ml/kg, no positive end-expiratory pressure) for minutes simulate room resuscitation, either with...
<h3>Importance</h3> Dexamethasone initiated after the first week of life reduces rate death or bronchopulmonary dysplasia (BPD) but may cause long-term adverse effects in very preterm infants. Hydrocortisone is increasingly used as an alternative, evidence supporting its efficacy and safety lacking. <h3>Objective</h3> To assess effect hydrocortisone between 7 14 days birth on BPD <h3>Design, Setting, Participants</h3> Double-blind, placebo-controlled randomized trial conducted 19 neonatal...
The identification of independent clinical risk factors for necrotizing enterocolitis (NEC) may contribute to early selection infants at risk, allowing the development targeted strategies aimed prevention NEC.The objective this study was identify contributing NEC in a large multicenter cohort.This prospective cohort performed 9 neonatal intensive care units. Infants born gestational age ≤30 weeks were included. Demographic and data collected daily until day 28 postnatally. Factors predictive...
Chorioamnionitis is the most significant source of prenatal inflammation and preterm delivery. Prematurity are associated with compromised postnatal developmental outcomes, intestinal immune defence, gut barrier function vascular system. We developed a sheep model to study how antenatal development was affected by gestation and/or endotoxin induced chorioamnionitis.Chorioamnionitis at different gestational ages (GA). Animals were sacrificed low GA after 2d or 14d exposure chorioamnionitis....
Abstract Background Hypoxic-ischemic encephalopathy (HIE) is one of the most important causes brain injury in preterm infants. Preterm HIE predominantly caused by global hypoxia-ischemia (HI). In contrast, focal ischemia common adult and known to result cerebral inflammation activation peripheral immune system. These inflammatory responses are considered play an role adverse outcomes following ischemia. this study, we hypothesize that also involved after HI. Methods instrumented fetal sheep...
The proinflammatory stimulus of chorioamnionitis is commonly associated with preterm delivery. Women at risk delivery receive antenatal glucocorticoids to functionally mature the fetal lung. However, effects combined exposures and on fetus are poorly understood. Time-mated ewes singleton fetuses received an intra-amniotic injection lipopolysaccharide (LPS) either preceding or following maternal intramuscular betamethasone 7 14 days before delivery, were delivered 120 gestational age (GA)...
Blood-brain barrier (BBB) disruption is associated with hypoxia-ischemia (HI) induced brain injury and life-long neurological pathologies. Treatment options are limited. Recently, we found that mesenchymal stem/stromal cell derived extracellular vesicles (MSC-EVs) protected the in ovine fetuses exposed to HI. We hypothesized Annexin A1 (ANXA1), present MSC-EVs, contributed their therapeutic potential by targeting ANXA1/Formyl peptide receptor (FPR), thereby preventing loss of BBB integrity....
Liquid-based perinatal life support (PLS) technology will probably be applied in a first-in-human study within the next decade. Research and development of PLS should not only address technical issues, but also consider socio-ethical legal aspects, its application area, corresponding design implications. This paper represents consensus opinion group healthcare professionals, designers, ethicists, researchers patient representatives, who have expertise tertiary obstetric neonatal care,...
Antenatal inflammation may be an important triggering event in the pathogenesis of bronchopulmonary dysplasia but also accelerate fetal lung maturation. We examined effects intra-amniotic (IA) interleukin (IL)-1 alpha and IL-1 beta on maturation sheep lung. These cytokine were compared with IA endotoxin, a potent proinflammatory stimulus that accelerated Date-bred ewes received 15 or 150 microg recombinant ovine 10 mg Escherichia coli endotoxin by injection at 118 days gestation (term =...
Antenatal betamethasone (Beta) is widely used in women with asymptomatic chorioamnionitis at risk for preterm delivery, but its effects on fetal inflammation are unstudied. Groups of ewes 109 ± 1 days gestation received the following treatments: intra-amniotic (IA) saline (control), 0.5 mg/kg intramuscular Beta, 10 mg IA endotoxin (Endo), and Beta + 2 h later Endo (Beta Endo). suppressed Endo-induced lung day. However, compared 5 after treatment, lambs had increased alveolar neutrophils,...
The fetal lung normally contains immature monocytes and very few mature macrophages. chorioamnionitis frequently associated with preterm birth induces monocyte influx into the lung. Previous studies demonstrated that in developing can mediate injury responses resemble BPD humans. We hypothesized would induce maturation of Groups three to seven time-mated ewes received saline or 10 mg endotoxin (Escherichia coli 055:B5) by intra-amniotic injection for intervals from 1 14 days before operative...