A5015144738- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Hematopoietic Stem Cell Transplantation
- Extracellular vesicles in disease
- Periodontal Regeneration and Treatments
- Angiogenesis and VEGF in Cancer
- MicroRNA in disease regulation
- Acute Myeloid Leukemia Research
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Skin and Cellular Biology Research
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Cancer, Hypoxia, and Metabolism
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- RNA Interference and Gene Delivery
- Platelet Disorders and Treatments
- Cancer Genomics and Diagnostics
- Wound Healing and Treatments
- Sarcoma Diagnosis and Treatment
- Hematological disorders and diagnostics
- RNA Research and Splicing
Paracelsus Medical University
2016-2025
University of Salzburg
2011-2024
Alfried Krupp Hospital
2023
Humboldt-Universität zu Berlin
1989-2019
Charité - Universitätsmedizin Berlin
2019
Freie Universität Berlin
2019
Rosalind Franklin University of Medicine and Science
2019
Medical University of Graz
2006-2015
Stem Cell Institute
2008-2014
Medizinische Hochschule Hannover
2012
Extracellular vesicles (EVs), such as exosomes and microvesicles, are released by different cell types participate in physiological pathophysiological processes. EVs mediate intercellular communication cell‐derived extracellular signalling organelles that transmit specific information from their of origin to target cells. As a result these properties, defined may serve novel tools for various therapeutic approaches, including (a) anti‐tumour therapy, (b) pathogen vaccination, (c)...
BACKGROUND: Human multipotent mesenchymal stromal cells (MSCs) are promising candidates for a growing spectrum of regenerative and immunomodulatory cellular therapies. Translation auspicious experimental results into clinical applications has been limited by the dependence MSC propagation from fetal bovine serum (FBS). STUDY DESIGN AND METHODS: The capacity human platelet lysate (HPL) to replace FBS clinical-scale was analyzed. RESULTS: HPL could be efficiently produced buffy coats....
Abstract Mesenchymal stromal cells (MSCs) are promising candidates for novel cell therapeutic applications. For clinical scale manufacturing, human factors from serum or platelets have been suggested as alternatives to fetal bovine (FBS). We previously shown that pooled (HS) and thrombin-activated platelet releasate in plasma (tPRP) support the expansion of adipose tissue-derived MSCs. Contradictory results with bone marrow (BM)-derived MSCs initiated a comprehensive comparison HS, tPRP,...
Abstract Nanoparticles can acquire a plasma protein corona defining their biological identity. Corona functions were previously considered for cell‐derived extracellular vesicles (EVs). Here we demonstrate that nano‐sized EVs from therapy‐grade human placental‐expanded (PLX) stromal cells are surrounded by an imageable and functional when enriched with permissive technology. Scalable EV separation cell‐secreted soluble factors via tangential flow‐filtration (TFF) subtractive tandem mass‐tag...
Abstract The generation of endothelial progenitor cells (EPCs) from blood monocytes has been propagated as a novel approach in the diagnosis and treatment cardiovascular diseases. Low-density lipoprotein (LDL) uptake lectin binding together with marker expression are commonly used to define these EPCs. Considerable controversy exists regarding their nature, particular, because myelomonocytic share several properties (ECs). This study was performed elucidate whether determination is...
Adult mesenchymal stem cells (MSCs) are considered as valuable mediators for tissue regeneration and cellular therapy. This study was performed to develop conditions regularly propagating a clinical quantity of >2 × 108 MSCs without animal serum from small bone marrow (BM) aspiration volumes within short time. We established optimized culture with pooled human platelet lysate (pHPL) replacing fetal bovine (FBS) MSC propagation. quality, identity, purity, function were assessed accordingly....
Evaluation of novel cellular therapies in large-animal models and patients is currently hampered by the lack imaging approaches that allow for long-term monitoring viable transplanted cells. In this study, sodium iodide symporter (NIS) transgene was evaluated as an approach to follow vivo survival, engraftment, distribution human-induced pluripotent stem cell (hiPSC) derivatives a pig model myocardial infarction.Transgenic hiPSC lines stably expressing fluorescent reporter NIS...
Background: Umbilical cord blood (UCB) is an easily accessible alternative source for multipotent mesenchymal stromal cells (MSCs) and generally believed to provide MSCs with a higher proliferative potential compared adult bone marrow. Limitations in cell number strict dependence of expansion procedures from selected lots fetal bovine serum have hampered the progress clinical applications UCB-derived MSCs. Methods: We analyzed isolation human UCB marrow under optimized ex vivo culture...
Endothelial progenitor cells (EPC) are considered powerful biologic markers for vascular function and cardiovascular risk, predicting events death from causes. Colony-forming units of endothelial (CFU-EC) used to quantify EPC circulating in human peripheral blood. The mechanisms underlying colony formation the nature contributing not clear. We performed subtractive CFU-EC analyses determine impact various blood cell types kinetics protein gene expression during formation. found that mainly...
Mesenchymal stromal cells (MSC) are currently tested in a large number of clinical trials and raise high hope regenerative medicine. These have to be expanded vitro before transplantation several studies demonstrated that long-term culture evokes continuous changes MSC: proliferation rate decays, the cell size increases, differentiation potential is affected, chromosomal instabilities may arise molecular acquired. Long-term preparations might also therapeutic consequences, although this has...
The inherent immunomodulatory capacity of mesenchymal stem/progenitor cells (MSPCs) encouraged initiation multiple clinical trials. Release criteria for therapeutic MSPCs cover identity, purity and safety but appropriate potency assessment is often missing. Reports on functional heterogeneity created additional uncertainty regarding donor organ/source selection. We established a robust immunomodulation assay based pooling responder leukocytes to minimize individual immune response...
Spinal cord injury is characterized by initial neural tissue disruption that triggers secondary damage and extensive non-resolving inflammation, which aggravates loss of function hinders recovery. The early onset inflammation following traumatic spinal underscores the importance acute intervention after trauma. Injections mesenchymal stromal cells (MSCs) can reduce injury. We asked if extracellular vesicles (EVs) substitute anti-inflammatory anti-scarring activities their parental MSCs in a...