A5005728573- Wound Healing and Treatments
- Mesenchymal stem cell research
- Craniofacial Disorders and Treatments
- Cleft Lip and Palate Research
- Tissue Engineering and Regenerative Medicine
- Dermatologic Treatments and Research
- Reconstructive Surgery and Microvascular Techniques
- Surgical Sutures and Adhesives
- Bone fractures and treatments
- Electrospun Nanofibers in Biomedical Applications
- dental development and anomalies
- Tendon Structure and Treatment
- Pluripotent Stem Cells Research
- Connective tissue disorders research
- Body Contouring and Surgery
- Cancer Cells and Metastasis
- Biomedical Ethics and Regulation
- Fibroblast Growth Factor Research
- Cellular Mechanics and Interactions
- Periodontal Regeneration and Treatments
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Effects of Radiation Exposure
- TGF-β signaling in diseases
- Angiogenesis and VEGF in Cancer
Stanford University
2016-2025
Stanford Medicine
2015-2024
California Institute for Regenerative Medicine
2015-2024
Palo Alto University
2011-2024
Institute for Stem Cell Biology and Regenerative Medicine
2014-2023
Creative Commons
2016-2023
Stanford Health Care
2003-2023
Menlo School
2013-2022
University of Virginia
2008-2022
BG University Hospital Bergmannsheil Bochum
2022
Recent in situ hybridization studies had demonstrated a strong increase vascular endothelial growth factor (VEGF) mRNA expression the hyperproliferative epithelium during wound healing. To determine potential mediators of VEGF induction this process, we analyzed regulation cultured human keratinocytes. We found large upon treatment quiescent cells with serum, epidermal factor, transforming factor-β1, keratinocyte or proinflammatory cytokine tumor necrosis α, respectively. Since all these...
Human induced pluripotent stem cells generated from patients with familial dilated cardiomyopathy model cardiovascular disease in these patients.
Background Inadequate oxygen (hypoxia) triggers a multifaceted cellular response that has important roles in normal physiology and many human diseases. A transcription factor, hypoxia-inducible factor (HIF), plays central role the hypoxia response; its activity is regulated by oxygen-dependent degradation of HIF-1α protein. Despite ubiquity importance responses, little known about variation global transcriptional to among different cell types or how this might relate tissue- cell-specific...
Recent studies have shown that application of basic fibroblast growth factor (basic FGF) to a wound has beneficial effect. However, it not been assessed whether endogenous FGF also plays role in tissue repair. In this study we found 160-fold induction mRNA encoding keratinocyte (KGF) 1 day after skin injury. This large was unique within the family FGFs, since levels acidic FGF, and FGF-5 were only slightly induced (2- 10-fold) during healing, there no expression FGF-3, FGF-4, FGF-6 detected...
The function of keratinocyte growth factor (KGF) in normal and wounded skin was assessed by expression a dominant-negative KGF receptor transgene basal keratinocytes. transgenic mice characterized epidermal atrophy, abnormalities the hair follicles, dermal hyperthickening. Upon injury, inhibition signaling reduced proliferation rate keratinocytes at wound edge, resulting substantially delayed reepithelialization wound.
Dermal fibroblasts represent a heterogeneous population of cells with diverse features that remain largely undefined. We reveal the presence at least two fibroblast lineages in murine dorsal skin. Lineage tracing and transplantation assays demonstrate single lineage is responsible for bulk connective tissue deposition during embryonic development, cutaneous wound healing, radiation fibrosis, cancer stroma formation. Lineage-specific cell ablation leads to diminished wounds reduces melanoma...
Ectopic expression of transcription factors can reprogram somatic cells to a pluripotent state. However, most the studies used skin fibroblasts as starting population for reprogramming, which usually take weeks expansion from single biopsy. We show here that induced stem (iPS) be generated adult human adipose (hASCs) freshly isolated patients. Furthermore, iPS readily derived hASCs in feeder-free condition, thereby eliminating potential variability caused by using feeder cells. safely and...
Hypertrophic scars occur following cutaneous wounding and result in severe functional esthetic defects. The pathophysiology of this process remains unknown. Here, we demonstrate for the first time that mechanical stress applied to a healing wound is sufficient produce hypertrophic mice. resulting are histopathologically identical human persist more than six months brief (one-week) period augmented during proliferative phase healing. Resulting structurally showed dramatic increases volume...
Skin scarring, the end result of adult wound healing, is detrimental to tissue form and function. Engrailed-1 lineage-positive fibroblasts (EPFs) are known function in but lineage-negative (ENFs) remain poorly characterized. Using cell transplantation transgenic mouse models, we identified a dermal ENF subpopulation that gives rise postnatally derived EPFs by activating expression during healing. By studying responses substrate mechanics, found mechanical tension drives activation via...