A5035837228- Inflammatory Myopathies and Dermatomyositis
- Immunotherapy and Immune Responses
- Rheumatoid Arthritis Research and Therapies
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Eosinophilic Disorders and Syndromes
- Immune Response and Inflammation
- Immunodeficiency and Autoimmune Disorders
- T-cell and B-cell Immunology
- Systemic Sclerosis and Related Diseases
- Systemic Lupus Erythematosus Research
- Atherosclerosis and Cardiovascular Diseases
- Lymphoma Diagnosis and Treatment
- Virus-based gene therapy research
- Virology and Viral Diseases
- SARS-CoV-2 and COVID-19 Research
- Immune cells in cancer
- HIV Research and Treatment
- Chronic Lymphocytic Leukemia Research
- COVID-19 Clinical Research Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Mosquito-borne diseases and control
- Cell death mechanisms and regulation
- Inflammasome and immune disorders
- Alcoholism and Thiamine Deficiency
Université Claude Bernard Lyon 1
2006-2025
Hospices Civils de Lyon
2017-2025
Hôpital Edouard Herriot
2019-2024
Hôpital Lyon Sud
2017-2024
HCL Technologies (India)
2024
Institut thématique Immunologie, inflammation, infectiologie et microbiologie
2021-2023
Inserm
2001-2022
Université de Lyon
2019
Centre Hospitalier Universitaire de Clermont-Ferrand
2018
Université Clermont Auvergne
2016
Systemic anticancer chemotherapy is immunosuppressive and mostly induces nonimmunogenic tumor cell death. Here, we show that even in the absence of any adjuvant, cells dying response to anthracyclins can elicit an effective antitumor immune suppresses growth inoculated tumors or leads regression established neoplasia. Although both antracyclins mitomycin C induced apoptosis with caspase activation, only anthracyclin-induced immunogenic death was immunogenic. Caspase inhibition by Z-VAD-fmk...
Abstract Proinflammatory oxidized phospholipids are generated during oxidative modification of low-density lipoproteins (LDL). The production these proinflammatory is controlled by secreted enzymes that circulate as proteins complexed with LDL and high-density lipoprotein. During the acute phase response to tissue injury, profound changes occur in lipoprotein enzymatic composition alter their anti-inflammatory function. Monocytes may encounter vivo differentiation macrophages or dendritic...
Abstract The compound 1-methyl-tryptophan (1-MT) is a competitive inhibitor of IDO that can break tolerance and induce fetus, graft, tumor rejection. Because its broad effect on immune-related mechanisms, the direct action 1-MT human monocyte-derived dendritic cells (DC) was analyzed. It shown here DC dependent maturation pathway. Although had no stimulated by TLR3 ligand poly(I:C), it strongly enhanced Th1 profile with TLR2/1 or TLR2/6 ligands. Drastic changes in function TLR4 LPS were...
Anti-melanoma differentiation-associated gene 5 (MDA5) antibody (Ab) positive dermatomyositis (anti-MDA5 DM) is a rare entity associated with poor prognosis and multiple immunologic abnormalities. These include the presence of autoAbs deleterious interferon (IFN)-gamma production in severe form disease. Here, we show that profile differs between patients, depending on disease severity, from B cells patients directly stimulate IFN-gamma by peripheral blood cells. Serum 29 anti-MDA5 DM were...
To discover new and detect known antisynthetase autoantibodies (ASAs) through protein immunoprecipitation combined with gel-free liquid chromatography-tandem mass spectrometry (IP-MS).IP-MS was performed using sera of individuals showing features syndrome (ASyS) without (n=5) (n=12) previously detected ASAs, healthy controls (n=4). New candidate aminoacyl-tRNA-synthetase (ARS) autoantigens identified unbiased IP-MS were confirmed by IP-western blot. A targeted assay for various ASA...
Abstract During the acute phase response, interplay between high density lipoproteins and low (LDL) favors transient generation of oxidized LDL with proinflammatory activities. We hypothesized that oxidative modification is an endogenous signal for immune system, we have shown promotes mature dendritic cell transition from monocyte, therefore linking nonspecific response to adaptive immunity. Lysophosphatidylcholine (LPC) a major lipid component reported now report LPC acts through G...
Lentiviral vectors are under intense scrutiny as unique candidate viral vector vaccines against tumor and aggressive pathogens because of their ability to initiate potent durable specific immune responses. Strategies that alleviate safety concerns will facilitate the clinical developments involving lentiviral vectors. In this respect, development integration deficient circumvents relative insertional mutagenesis might pave way for applications in which gene transfer is targeted non-dividing...
Serological studies of COVID-19 convalescent patients have identified polyclonal lineage-specific and cross-reactive antibodies (Abs), with varying effector functions against virus variants. Individual specificities anti-SARS-CoV-2 Abs their impact on infectivity by other variants been little investigated to date. Here, we dissected at a monoclonal level neutralizing enhancing elicited early how they affect emerging B cells from 13 originally infected D614G or Alpha were immortalized isolate...
Abstract High level of phospholipase A 2 (PLA ) activity is found in serum and biological fluids during the acute‐phase response (APR). Extracellular PLA patients with inflammatory diseases such as sepsis, acute pancreatitis or rheumatoid arthritis also associated propagation inflammation. involved release bothpro‐ anti‐inflammatory lipid mediators from phospholipids cellular membranes circulating lipoproteins. may thus generate signals that influence immune responses. Here, groupIII...
AIDS vaccination has a pressing need for more potent vectors capable of eliciting strong, diversified, and long-lasting cellular immune responses against human immunodeficiency virus (HIV). Lentiviral have demonstrated efficiency not only as gene delivery vehicles therapy applications but also tools. This is likely due to their ability transduce nondividing cells, including dendritic enabling sustained endogenous antigen presentation thus the induction high proportions specific cytotoxic T...
Background: Antibodies against Ku have been described in patients with various connective tissue diseases. The objective of this study was to describe the clinical, functional, and imaging characteristics interstitial lung disease anti-Ku antibodies. Methods: This single-center, retrospective observational conducted at a tertiary referral institution. Patients positive antibodies identified between 2007 2022 were included. Clinical, immunological, data systematically reviewed. Results:...
Because of its oxidative modification during the acute-phase response to an aggression, low density lipoprotein (LDL) can be regarded as a source lipid mediators that act both promote and inhibit inflammation. This exemplified by production anti-inflammatory oxidized fatty acids proinflammatory lysophosphatidylcholine (LPC) LDL oxidation. We have shown previously (oxLDL) plays active role at interface between innate adaptive immunity delivering instructive molecules such LPC, which promotes...
Dear Editor, Anti-eukaryotic initiation factor 2B (eIF2B) autoantibodies are a recently described autoantibody specificity in SSc associated with diffuse cutaneous involvement, interstitial lung disease (ILD) and cytoplasmic pattern on HEp-2 indirect immunofluorescence (HEp-2 IIF) [1–3]. Hitherto only 19 patients have been identified, all by radiolabelled protein immunoprecipitation Here we describe immunoprecipitation-mass spectrometry (IP-MS) as detection method for anti-eIF2B further...
Trials testing the RTS,S candidate malaria vaccine and radiation-attenuated sporozoites (RAS) have shown that protective immunity against can be induced an effective is not out of reach. However, longer-term protection higher rates are required to eradicate from endemic regions. It implies there still a need explore new strategies. Lentiviral vectors very potent at inducing strong immunological memory. However their integrative status challenges safety profile. Eliminating integration step...
Inflammatory monocyte-derived dendritic cells (Mo-DCs) have been described in several chronic inflammatory disorders, such as rheumatoid arthritis (RA), and are suspected to play a detrimental role by fueling inflammation skewing adaptive immune responses. However, the characterization of their phenotype is still limited, well comprehension factors that govern differentiation. Here, we show Mo-DCs generated vitro expressed large atypical panel C-type lectin receptors, including isoforms...
The objective of this study was to develop a serum biochemical marker the degradation type III and IV collagens, as an index synovium turnover, evaluate its performance in patients with rheumatoid arthritis (RA). An enzyme-linked immunosorbent assay for synovial collagen fragments (Col3-4) developed using antibody recognizing specific sequence from human collagen, which shares 70% homology collagen. Immunohistochemistry performed localize Col3-4 matrix metalloprotease MMP-9 is upregulated RA...