A5012394244- Influenza Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- HIV Research and Treatment
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- interferon and immune responses
- Mosquito-borne diseases and control
- RNA and protein synthesis mechanisms
- Long-Term Effects of COVID-19
- Viral Infections and Vectors
- RNA Research and Splicing
- HIV/AIDS drug development and treatment
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- RNA modifications and cancer
- Virology and Viral Diseases
- Olfactory and Sensory Function Studies
- SARS-CoV-2 detection and testing
- Viral Infections and Outbreaks Research
- Bacteriophages and microbial interactions
- HIV-related health complications and treatments
- Animal Disease Management and Epidemiology
- Neuroinflammation and Neurodegeneration Mechanisms
Centre National de la Recherche Scientifique
2014-2025
Université Paris Cité
2014-2025
Institut Pasteur
2014-2025
Université Paris-Saclay
2023
Centre de Gestion Scientifique
2021
Centre National pour la Recherche Scientifique et Technique (CNRST)
2020
Sorbonne Paris Cité
2011-2017
Délégation Paris 7
2010-2017
Université Claude Bernard Lyon 1
2009
Institut Cochin
2005
Summary Background The Oise department in France has been heavily affected by COVID-19 early 2020. Methods Between 30 March and 4 April 2020, we conducted a retrospective closed cohort study among pupils, their parents siblings, as well teachers non-teaching staff of high-school located Oise. Participants completed questionnaire that covered history fever and/or respiratory symptoms since 13 January 2020 had blood tested for the presence anti-SARS-CoV-2 antibodies. infection attack rate...
A deletion of about 20 amino acids in the stalk neuraminidase (NA) is frequently detected upon transmission influenza viruses from waterfowl to domestic poultry. Using reverse genetics, a recombinant virus derived wild duck isolate, A/Mallard/Marquenterre/Z237/83 (MZ), and an NA variant (MZ-delNA) were produced. Compared type, MZ-delNA showed moderate growth advantage on avian cultured cells. In 4-week-old chickens inoculated intratracheally with virus, viral replication lungs, liver,...
Background Children’s role in SARS-CoV-2 epidemiology remains unclear. We investigated an initially unnoticed outbreak linked to schools northern France, beginning as early mid-January 2020. Aims This retrospective observational study documents the extent of transmission, affected high school (n = 664 participants) and primary 1,340 participants), context unsuspected circulation limited control measures. Methods Between 30 March April 2020, all staff, well pupils their parents relatives were...
Background: The Oise department in France has been heavily affected by COVID-19 early 2020.Methods: Between 30 March and 4 April 2020, we conducted a retrospective closed cohort study among pupils, their parents siblings, as well teachers non-teaching staff of high-school located Oise. Participants completed questionnaire that covered history fever and/or respiratory symptoms since 13 January 2020 had blood tested for the presence anti-SARS-CoV-2 antibodies. infection attack rate (IAR) was...
Abstract The animal reservoir of SARS-CoV-2 is unknown despite reports various SARS-CoV-2-related viruses in Asian Rhinolophus bats, including the closest virus from R. affinis, RaTG13. Several studies have suggested involvement pangolin coronaviruses emergence. presents a mosaic genome, to which different progenitors contribute. spike sequence determines binding affinity and accessibility its receptor-binding domain (RBD) cellular angiotensin-converting enzyme 2 (ACE2) receptor responsible...
Abstract Anosmia was identified as a hallmark of COVID-19 early in the pandemic, however, with emergence variants concern, clinical profile induced by SARS-CoV-2 infection has changed, anosmia being less frequent. Here, we assessed clinical, olfactory and neuroinflammatory conditions golden hamsters infected original Wuhan strain, its isogenic ORF7-deletion mutant three variants: Gamma, Delta, Omicron/BA.1. We show that animals develop variant-dependent disease including anosmia, ORF7...
ABSTRACT Studies on the intracellular trafficking of influenza virus ribonucleoproteins are currently limited by lack a method enabling their visualization during infection in single cells. This is largely due to difficulty encoding fluorescent fusion proteins within viral genome. To circumvent this limitation, we used split-green protein (split-GFP) system (S. Cabantous, T. C. Terwilliger, and G. S. Waldo, Nat. Biotechnol. 23:102–107, 2005) produce quasi-wild-type recombinant...
After cell entry, HIV undergoes rapid transport toward the nucleus using microtubules and microfilaments. Neither cellular cytoplasmic components nor viral proteins that interact to mediate have yet been identified. Using a yeast two-hybrid screen, we identified four cytoskeletal as putative interaction partners for HIV-1 p24 capsid protein: MAP1A, MAP1S, CKAP1, WIRE. Depletion of MAP1A/MAP1S in indicator lines primary human macrophages led profound reduction infectivity result impaired...
Interferon restricts SARS-CoV-2 replication in cell culture, but only a handful of Stimulated Genes with antiviral activity against have been identified. Here, we describe functional CRISPR/Cas9 screen aiming at identifying restriction factors. We identify DAXX, scaffold protein residing PML nuclear bodies known to limit the DNA viruses and retroviruses, as potent inhibitor SARS-CoV human cells. Basal expression DAXX is sufficient SARS-CoV-2, over-expression further infection. an early,...
<title>Abstract</title> Recombination is a crucial process in the evolution of many organisms. Although evolutionary reasons behind its occurrence RNA viruses are debated, this phenomenon has been associated with major epidemiological events such as virus host range expansion, antigenic shift or variation virulence 1,2, and occurs frequently positive strand coronaviruses. The SARS-CoV-2 pandemic repeated emergence variants concern presenting increased transmissibility, severity immune escape...
Influenza A viruses are major pathogens in humans and animals, whose genome consists of eight single-stranded RNA segments negative polarity. Viral mRNAs synthesized by the viral RNA-dependent polymerase nucleus infected cells, close association with cellular transcriptional machinery. Two proteins essential for multiplication, exportin NS2/NEP ion channel protein M2, produced splicing NS1 M1 mRNAs, respectively. Here we identify two human spliceosomal factors, RED SMU1, that control...
Serological studies of COVID-19 convalescent patients have identified polyclonal lineage-specific and cross-reactive antibodies (Abs), with varying effector functions against virus variants. Individual specificities anti-SARS-CoV-2 Abs their impact on infectivity by other variants been little investigated to date. Here, we dissected at a monoclonal level neutralizing enhancing elicited early how they affect emerging B cells from 13 originally infected D614G or Alpha were immortalized isolate...
ABSTRACT A deletion of ∼20 amino acids in the stalk neuraminidase is frequently observed upon transmission influenza viruses from waterfowl to domestic poultry. pair recombinant H7N1 bearing either a short- or long-stalk was genetically engineered. Inoculation long-stalk-neuraminidase virus resulted higher cloacal excretion ducks and led conversely lower-level oropharyngeal chickens, associated with higher-level local immune response better survival. Therefore, short-stalk determinant viral...
A precise mapping of pathogen–host interactions is essential for comprehensive understanding the processes infection and pathogenesis. The most frequently used techniques interactomics are yeast two-hybrid binary methodologies, which do not recapitulate pathogen life cycle, tandem affinity purification mass spectrometry co-complex cannot distinguish direct from indirect interactions. New technologies thus needed to improve In current study, we detected between influenza virus polymerase host...
AIDS vaccination has a pressing need for more potent vectors capable of eliciting strong, diversified, and long-lasting cellular immune responses against human immunodeficiency virus (HIV). Lentiviral have demonstrated efficiency not only as gene delivery vehicles therapy applications but also tools. This is likely due to their ability transduce nondividing cells, including dendritic enabling sustained endogenous antigen presentation thus the induction high proportions specific cytotoxic T...
Abstract Enhancing the knowledge of host factors that are required for efficient influenza A virus (IAV) replication is essential to address questions related pathogenicity and identify targets antiviral drug development. Here we focused on interplay between IAV DExD-box RNA helicases (DDX), which play a key role in cellular metabolism by remodeling RNA-RNA or RNA-protein complexes. We performed targeted RNAi screen 35 human DDX proteins those involved life cycle. DDX19 was major hit. In...
Abstract An accurate spatial representation of protein-protein interaction networks is needed to achieve a realistic and biologically relevant interactomes. Here, we leveraged the information included in Proximity-Dependent Biotin Identification (BioID) interactomes SARS-CoV-2 proteins calculate weighted distances model organization SARS-CoV-2-human interactome three dimensions (3D) within cell-like volume. Cell regions with viral occupancy were highlighted, along coordination exploiting...
<title>Abstract</title> Influenza virus infections can cause severe complications such as Acute Necrotizing Encephalopathy (ANE), which is characterised by rapid onset pathological inflammation following febrile infection. Heterozygous dominant mutations in the nucleoporin RANBP2/Nup358 predispose to influenza-triggered ANE1. The aim of our study was determine whether RANBP2 plays a role IAV-triggered inflammatory responses. We found that depletion human airway epithelial cell line increased...
Besides the classical respiratory and systemic symptoms, unusual complications of influenza A infection in humans involve skeletal muscles. Numerous cases acute myopathy and/or rhabdomyolysis have been reported, particularly following outbreak pandemic A(H1N1) 2009. The pathogenesis these influenza-associated myopathies (IAM) remains unkown, although direct muscle cells is suspected. Here, we studied susceptibility cultured human primary to a 2009 2008 seasonal isolate. Using from different...
Background During the 2007–2008 season, A(H1N1) viruses naturally resistant to oseltamivir due an H275Y substitution in neuraminidase emerged and spread human population. The of has increased affinity for sialic acids as compared with N1 previously circulating viruses. Methods Using site-directed mutagenesis analysis enzymatic assay on cells transiently expressing viral neuraminidase, amino acid changes that could account particular properties were explored. substrate ( K m ) inhibition...