A5086161043- Neuroinflammation and Neurodegeneration Mechanisms
- Mosquito-borne diseases and control
- Neurological Disease Mechanisms and Treatments
- HIV Research and Treatment
- Studies on Chitinases and Chitosanases
- Bacteriophages and microbial interactions
- Listeria monocytogenes in Food Safety
- interferon and immune responses
- Muscle Physiology and Disorders
- RNA Research and Splicing
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Tryptophan and brain disorders
- Escherichia coli research studies
- Congenital heart defects research
- Cytomegalovirus and herpesvirus research
- Treatment of Major Depression
- Biosensors and Analytical Detection
- Cell Image Analysis Techniques
- Viral Infections and Outbreaks Research
- Genomics and Phylogenetic Studies
- Nuclear Structure and Function
- Tuberculosis Research and Epidemiology
- Trace Elements in Health
- Signaling Pathways in Disease
Institut Pasteur
2014-2024
Université Paris Cité
1988-2024
Laboratoire de Biologie, Bioingéniérie et Bioimagerie ostéoarticulaire
2022-2023
Biology of Infection
2019
Institut Textile et Chimique
2019
Human Technopole
2013
Eckert & Ziegler (United States)
2011
Biblioteca Nacional de España
2011
Escola da Cidade
2011
Éditions Gallimard (France)
2011
DNA methylation is an epigenetic mark thought to be robust environmental perturbations on a short time scale. Here, we challenge that view by demonstrating the infection of human dendritic cells (DCs) with live pathogenic bacteria associated rapid and active demethylation at thousands loci, independent cell division. We performed integrated analysis data genome-wide methylation, histone patterns, chromatin accessibility, gene expression, before after infection. found infection-induced rarely...
Mitochondria modulate Ca2+ signals by taking up, buffering, and releasing at key locations near release or influx channels. The role of such local interactions between channels organelles is difficult to establish in living cells because mitochondria form an interconnected network constantly remodeled coordinated fusion fission reactions. To study the effect a controlled disruption mitochondrial on homeostasis, we took advantage hFis1, protein that promotes recruiting dynamin-related...
Huntington's disease (HD) is a dominantly inherited neurodegenerative caused by CAG expansion in the huntingtin gene, which adds homopolymeric tract of polyglutamine (polyQ) to encoded protein leading formation toxic aggregates. Despite rapidly accumulating evidences supporting role for intercellular transmission aggregates, little known about whether and how (Htt) misfolding progresses through brain. It has been recently reported that synthetic polyQ peptides recombinant fragments mutant...
The nuclear pore complex (NPC) mediates nucleo-cytoplasmic transport of macromolecules and is an obligatory point passage functional bottleneck in the replication some viruses. Human Immunodeficiency Virus (HIV) has evolved required mechanisms for active import its genome through NPC. However by which NPC allows or even assists HIV translocation are still unknown. We investigated involvement four key nucleoporins HIV-1 docking, translocation, integration: Nup358/RanBP2, Nup214/CAN, Nup98...
Human neurons express the innate immune response receptor, Toll-like receptor 3 (TLR3). TLR3 levels are increased in pathological conditions such as brain virus infection. Here, we further investigated production, cellular localisation, and function of neuronal during neuronotropic rabies (RABV) infection human cells. Following RABV infection, is not only present endosomes, observed absence but also detergent-resistant perinuclear inclusion bodies. As well TLR3, these bodies contain viral...
Skeletal muscle has the capacity to adapt environmental changes and regenerate upon injury. To study these processes, most experimental methods use quantification of parameters obtained from images immunostained skeletal muscle. Muscle cross-sectional area, fiber typing, localization nuclei within fiber, number vessels, fiber-associated stem cells are used assess physiology. Manual is time consuming only poorly reproducible. While current state-of-the-art software tools unable analyze all...
Microglial cells are tissue-resident macrophages of the central nervous system. They extremely dynamic, sensitive to their microenvironment and present a characteristic complex heterogeneous morphology distribution within brain tissue. Many experimental clues highlight strong link between function in response aggression. However, due "dendritic-like" aspect that constitutes major pool murine microglial dense network, precise powerful morphological studies not easy realize complicate...
Escape into the host cell cytosol following invasion of mammalian cells is a common strategy used by invasive pathogens. This requires membrane rupture vesicular or vacuolar compartment formed around bacteria after uptake cell. The mechanism pathogen-induced disassembly poorly understood. We established novel, robust and sensitive fluorescence microscopy method that tracks precise time point vacuole upon Gram-negative bacteria. revealed enteroinvasive pathogen Shigella flexneri escapes...
Background Septins are conserved GTPases that form filaments and required in many organisms for several processes including cytokinesis. We previously identified SEPT9 associated with phagosomes containing latex beads coated the Listeria surface protein InlB. Methodology/Principal Findings Here, we investigated septin function during entry of invasive bacteria non-phagocytic mammalian cells. found SEPT9, its interacting partners SEPT2 SEPT11, recruited as collars next to actin at site...
Mycolactone is a diffusible lipid secreted by the human pathogen Mycobacterium ulcerans, which induces formation of open skin lesions referred to as Buruli ulcers. Here, we show that mycolactone operates hijacking Wiskott-Aldrich syndrome protein (WASP) family actin-nucleating factors. By disrupting WASP autoinhibition, leads uncontrolled activation ARP2/3-mediated assembly actin in cytoplasm. In epithelial cells, mycolactone-induced stimulation ARP2/3 concentrated perinuclear region,...
Major depressive disorder is a complex multifactorial condition with so far poorly characterized underlying pathophysiology. Consequently, the available treatments are from satisfactory as it estimated that up to 30% of patients resistant conventional treatment. Recent comprehensive evidence has been accumulated which suggests inflammation may be implied in etiology this disease. Here we investigated ketamine an innovative treatment strategy due its immune-modulating capacities. In murine...
Helicobacter pylori (Hp) is a major risk factor for gastric cancer (GC). Hp promotes DNA damage and proteasomal degradation of p53, the guardian genome stability. reduces expression transcription USF1 shown to stabilise p53 in response genotoxic stress. We investigated whether Hp-mediated deregulation impacts p53-response consequently genetic instability. also explored vivo role carcinogenesis.Human epithelial cell lines were infected with Hp7.13, exposed or not DNA-damaging agent...
Significance During embryonic development, multipotent stem cells progressively acquire specific cell fates. The somite is an embryological structure that gives rise to different mesodermal types, including skeletal muscle and vascular of blood vessels. We show by genetic manipulation the Notch signaling pathway promotes a cell-fate choice at expense in mouse somite. Pax3 + adjacent somites give myogenic endothelial limbs. Gain-of-function or inhibition affects this prior migration these...
After cell entry, HIV undergoes rapid transport toward the nucleus using microtubules and microfilaments. Neither cellular cytoplasmic components nor viral proteins that interact to mediate have yet been identified. Using a yeast two-hybrid screen, we identified four cytoskeletal as putative interaction partners for HIV-1 p24 capsid protein: MAP1A, MAP1S, CKAP1, WIRE. Depletion of MAP1A/MAP1S in indicator lines primary human macrophages led profound reduction infectivity result impaired...
Contactin genes CNTN5 and CNTN6 code for neuronal cell adhesion molecules that promote neurite outgrowth in sensory-motor pathways. Mutations of have previously been reported individuals with autism spectrum disorders (ASDs), but very little is known on their prevalence clinical impact. In this study, we identified deleterious variants ASD. Among the carriers, a girl ASD attention-deficit/hyperactivity disorder was carrying five copies CNTN5. For CNTN6, both deletions (6/1534 vs 1/8936...
Abstract Hepatic fibrosis is a major consequence of chronic liver disease such as non-alcoholic steatohepatitis which undergoing dramatic evolution given the obesity progression worldwide, and has no treatment to date. stellate cells (HSCs) play key role in process, because damage, they transdifferentiate from “quiescent” an “activated” phenotype responsible for most collagen deposition tissue. Here, using diet-induced murine model (choline-deficient amino acid-defined, high fat diet), we...
Abstract The synaptic protein SHANK3 encodes a multidomain scaffold expressed at the postsynaptic density of neuronal excitatory synapses. We previously identified de novo mutations in patients with autism spectrum disorders (ASD) and showed that represents one major genes for ASD. Here, we analyzed pyramidal cortical neurons derived from induced pluripotent stem cells four ASD carrying truncating mutations. At 40–45 days after differentiation neural cells, dendritic spines presented...
Background/Objectives Human leishmaniases are parasitic diseases causing severe morbidity and mortality. No vaccine is available numerous factors limit the use of current therapies. There thus an urgent need for innovative initiatives to identify new chemotypes displaying selective activity against intracellular Leishmania amastigotes that develop proliferate inside macrophages, thereby pathology leishmaniasis. Methodology/Principal Findings We have developed a biologically sound High...
Antigen recognition within immunological synapses triggers and sustains T cell activation by nucleating protein microclusters that gather receptors (TCRs), kinases, adaptors. Dissipation of these results in signal termination, but how this process is regulated unclear. In paper, we reveal release the adaptors SLP76 GADS from signaling induced serine/threonine kinase HPK1 phosphorylation plays a major role process. We found was recruited into triggered their dissipation inducing...