A5068547078- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Genomics and Chromatin Dynamics
- T-cell and B-cell Immunology
- RNA Research and Splicing
- Immune Cell Function and Interaction
- Neuroinflammation and Neurodegeneration Mechanisms
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Kruppel-like factors research
- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- Tendon Structure and Treatment
- RNA and protein synthesis mechanisms
- 14-3-3 protein interactions
- Pluripotent Stem Cells Research
- NF-κB Signaling Pathways
- Genetic Syndromes and Imprinting
- Neurogenesis and neuroplasticity mechanisms
- Estrogen and related hormone effects
- Neonatal Respiratory Health Research
- Autoimmune and Inflammatory Disorders Research
- Chemokine receptors and signaling
- RNA modifications and cancer
- Congenital heart defects research
Weizmann Institute of Science
2015-2025
University of Washington
2020-2022
Rappaport Family Institute for Research in the Medical Sciences
2011
Technion – Israel Institute of Technology
2011
Israel Institute
2011
Microglia are embryonically seeded macrophages that contribute to brain development, homeostasis, and pathologies. It is thus essential decipher how microglial properties temporally regulated by intrinsic extrinsic factors, such as sexual identity the microbiome. Here, we found microglia undergo differentiation phases, discernable transcriptomic signatures chromatin accessibility landscapes, which can diverge in adult males females. Remarkably, absence of microbiome germ-free mice had a time...
The genomics of human development Understanding the trajectory a developing requires an understanding how genes are regulated and expressed. Two papers now present pooled approach using three levels combinatorial indexing to examine single-cell gene expression chromatin landscapes from 15 organs in fetal samples. Cao et al. focus on measurements RNA broadly distributed cell types provide insights into organ specificity. Domcke examined accessibility cells these identify regulatory elements...
Drosophila melanogaster is a powerful, long-standing model for metazoan development and gene regulation. We profiled chromatin accessibility in almost 1 million expression half nuclei from overlapping windows spanning the entirety of embryogenesis. Leveraging developmental asynchronicity within embryo collections, we applied deep neural networks to infer age each nucleus, resulting continuous, multimodal views molecular cellular transitions absolute time. identify cell lineages; their...
Significance CD74 has been associated with tumor progression and metastasis. Its expression suggested to serve as a prognostic factor in many cancers, higher relative behaving marker of progression. Our previous studies showed that stimulation expressed on chronic lymphocytic cells initiates signaling cascade leading survival. The present study demonstrates CD74’s cytoplasmic domain binds chromatin regulates transcription genes involved immune regulation, cell survival, hematopoietic...
The mechanical challenge of attaching elastic tendons to stiff bones is solved by the formation a unique transitional tissue. Here, we show that murine tendon-to-bone attachment cells are bi-fated, activating mixture chondrocyte and tenocyte transcriptomes, under regulation shared regulatory elements Krüppel-like factors (KLFs) transcription factors. High-throughput bulk single-cell RNA sequencing humeral revealed expression hundreds chondrogenic tenogenic genes, which was validated in situ...
Antigen recognition within immunological synapses triggers and sustains T cell activation by nucleating protein microclusters that gather receptors (TCRs), kinases, adaptors. Dissipation of these results in signal termination, but how this process is regulated unclear. In paper, we reveal release the adaptors SLP76 GADS from signaling induced serine/threonine kinase HPK1 phosphorylation plays a major role process. We found was recruited into triggered their dissipation inducing...
Background: Rift Valley fever virus (RVFV) is a zoonotic that poses significant threat to both livestock and human health has caused outbreaks in endemic regions. In humans, most patients experience febrile illness; however, some patients, RVF disease may result hemorrhagic fever, retinitis, or encephalitis. While several veterinary vaccines are being utilized countries, currently, there no licensed therapeutics for use. Neutralizing antibodies specifically targeting vulnerable pathogen...
Approximately 50% of prostate cancer (PCa) patients harbor fusions involving the TMPRSS2 and ERG genes. Despite this, tailored therapies targeting fused gene, tERG, remain undeveloped. Our study analyzed biopsy samples from two clinical trials assessing efficacies androgen receptor (AR) signaling inhibitors (ARSIs). The results revealed that tERG promotes resistance to ARSIs is associated with elevated levels glucocorticoid (GR). Subsequent assays showed GR directly interacts alleviates...
Abstract Activation of endothelial cells by IL-1β triggers the expression multiple inflammatory cytokines and leukocyte-attracting chemokines. The machineries involved in secretion these inducible proteins are poorly understood. With use genome-wide transcriptional analysis inflamed human dermal microvascular cells, we identified several IL-1β−induced candidate regulators chose to focus our study on TNF-α-induced protein 2 (myeloid-secretory). silencing myeloid-secretory did not affect...
The bone marrow hosts NK cells whose distribution, motility and response to systemic immune challenge are poorly understood. At steady state, two-photon microscopy of the in Ncr1gfp/+ mice captured motile interacting with dendritic cells. expressed markers effector molecules mature Following poly (I:C) injection, RNA-Seq revealed three phases transcription featuring genes followed by posttranscriptional processes proliferation. Functionally, promoted upregulation granzyme B, enhanced...
The establishment of long-lasting immunity against pathogens is facilitated by the germinal center (GC) reaction, during which B cells increase their antibody affinity and differentiate into antibody-secreting (ASC) memory cells. These events involve modifications in chromatin packaging that orchestrate profound restructuring gene expression networks determine cell fate. While several remodelers were implicated lymphocyte functions, less known about SMARCA5. Here, using ribosomal pull-down...