A5029800498- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Cancer-related gene regulation
- Medical Image Segmentation Techniques
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Brain Tumor Detection and Classification
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Animal Genetics and Reproduction
- Genetics and Neurodevelopmental Disorders
- Zebrafish Biomedical Research Applications
- 3D Printing in Biomedical Research
- Reproductive Biology and Fertility
- Bioinformatics and Genomic Networks
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Neural Network Applications
- AI in cancer detection
- Ubiquitin and proteasome pathways
- Gene expression and cancer classification
- Image Processing Techniques and Applications
- Inflammatory Myopathies and Dermatomyositis
- Protein Kinase Regulation and GTPase Signaling
Weizmann Institute of Science
2015-2025
Hebrew University of Jerusalem
2007-2011
Broad Institute
2011
Massachusetts Institute of Technology
2011
Howard Hughes Medical Institute
2011
mRNA modification regulates pluripotency When stem cells progress from an embryonic pluripotent state toward a particular lineage, molecular switches dismantle the transcription factor network that keeps cell pluripotent. Geula et al. now show N6-methyladenosine (m6A), messenger RNA (mRNA) present on transcripts of factors, drives this transition. Methylation destabilized and limited their translation efficiency, which promoted timely decay naïve pluripotency. This m6A methylation was also...
The N6-methyladenosine (m 6 A) modification is the most prevalent post-transcriptional mRNA modification, regulating decay and splicing. It plays a major role during normal development, differentiation, disease progression. regulated by set of writer, eraser, reader proteins. YTH domain family proteins consists three homologous m A-binding proteins, Ythdf1, Ythdf2, Ythdf3, which were suggested to have different cellular functions. However, their sequence similarity tendency bind same targets...
In vitro cultured stem cells with distinct developmental capacities can contribute to embryonic or extraembryonic tissues after microinjection into pre-implantation mammalian embryos. However, whether independently give rise entire gastrulating embryo-like structures and compartments remains unknown. Here, we adapt a recently established platform for prolonged ex utero growth of natural embryos generate mouse post-gastrulation synthetic whole embryo models (sEmbryos), both compartments,...
Isolating human MEK/ERK signaling-independent pluripotent stem cells (PSCs) with naive pluripotency characteristics while maintaining differentiation competence and (epi)genetic integrity remains challenging. Here, we engineer reporter systems that allow the screening for defined conditions induce molecular functional features of pluripotency. Synergistic inhibition WNT/β-CATENIN, protein kinase C (PKC), SRC signaling consolidates induction teratoma-competent PSCs, capacity to differentiate...
The ability to study human post-implantation development remains limited owing ethical and technical challenges associated with intrauterine after implantation1. Embryo-like models spatially organized morphogenesis structure of all defining embryonic extra-embryonic tissues the conceptus (that is, disc, bilaminar yolk sac, chorionic sac surrounding trophoblast layer) remain lacking1,2. Mouse naive stem cells have recently been shown give rise capable self-assembling into post-gastrulation...
The recent derivation of human trophoblast stem cells (TSCs) from placental cytotrophoblasts and blastocysts opened opportunities for studying the development function placenta. Recent reports have suggested that naïve, but not primed, pluripotent (PSCs) retain an exclusive potential to generate TSCs. Here we report that, in absence WNT stimulation, transforming growth factor β (TGF-β) pathway inhibition leads direct robust conversion primed PSCs into resulting PSC-derived TSC lines exhibit...
Abstract Our ability to study early human post-implantation development remains highly limited due the ethical and technical challenges associated with intrauterine of embryo after implantation. Despite great progress made on gastruloids, axioloids in vitro cultured blastoids, such elegant models do not constitute an integrated Stem cell-derived Embryo Models (SEMs) that includes all key extra-embryonic tissues conceptus (e.g., hypoblast, yolk-sac, trophoblasts, amnion, extraembryonic...
The prevalence and morbidity of asthma, a chronic inflammatory airway disease, is increasing. Animal models provide meaningful but limited view the mechanisms asthma in humans. A systems-level that integrates multiple levels molecular functional information needed. For this, we compiled gene expression compendium from five publicly available mouse microarray datasets knowledge base 4,305 annotation sets. Using this collection generated high-level map themes characterize animal dominated by...
Hereditary inclusion body myopathy (HIBM) is an adult onset, slowly progressive distal and proximal myopathy. Although the causing gene, GNE, encodes for a key enzyme in biosynthesis of sialic acid, its primary function HIBM remains unknown. To elucidate pathological mechanisms leading from mutated GNE to phenotype, we attempted identify characterize early occurring downstream events by analyzing genomic expression patterns muscle specimens 10 patients carrying M712T Persian Jewish founder...
Abstract Motivation: Deciphering the complex mechanisms by which regulatory networks control gene expression remains a major challenge. While some studies infer regulation from dependencies between levels of putative regulators and their targets, others focus on measured physical interactions. Results: Here, we present Physical Module Networks, unified framework that combines Bayesian model describing modules co-expressed genes shared programs, interaction graph, protein–protein interactions...
Abstract The N6-methyladenosine (m 6 A) modification is the most prevalent post-transcriptional mRNA modification, regulating decay, translation and splicing. It plays a major role during normal development, differentiation, disease progression. dynamically regulated by set of writer, eraser reader proteins. YTH-domain family proteins: Ythdf1, Ythdf2, Ythdf3, are three homologous m A binding proteins, which have different cellular functions. However, their sequence similarity tendency to...