A5006003266- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Toxin Mechanisms and Immunotoxins
- Transgenic Plants and Applications
- Bacillus and Francisella bacterial research
- SARS-CoV-2 detection and testing
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- COVID-19 Clinical Research Studies
- Herpesvirus Infections and Treatments
- Neuroblastoma Research and Treatments
- vaccines and immunoinformatics approaches
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Poxvirus research and outbreaks
- Biosensors and Analytical Detection
- Chemical Synthesis and Analysis
- Long-Term Effects of COVID-19
- Microfluidic and Bio-sensing Technologies
- Computational Drug Discovery Methods
- Advanced Biosensing Techniques and Applications
- Neurological disorders and treatments
- Viral Infections and Outbreaks Research
Israel Institute for Biological Research
2016-2025
Laboratory of Molecular Genetics
2021
Abstract The novel highly transmissible human coronavirus SARS-CoV-2 is the causative agent of COVID-19 pandemic. Thus far, there no approved therapeutic drug specifically targeting this emerging virus. Here we report isolation and characterization a panel neutralizing monoclonal antibodies receptor binding domain (RBD). These were selected from phage display library constructed using peripheral circulatory lymphocytes collected patients at acute phase disease. are shown to recognize...
Abstract The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome 2 (SARS-CoV-2), exhibits high levels of mortality and morbidity has dramatic consequences on human life, sociality global economy. Neutralizing antibodies constitute a highly promising approach for treating preventing infection this novel pathogen. In present study, we characterize further evaluate recently identified monoclonal MD65 antibody its ability to provide protection against...
Public health experts emphasize the need for quick, point-of-care SARS-CoV-2 detection as an effective strategy controlling virus spread. To this end, many "antigen" devices were developed and commercialized. These are mostly based on detecting SARS-CoV-2's nucleocapsid protein. Recently, alerts issued by both FDA CDC raised concerns regarding devices' tendency to exhibit false positive results. In work, we a novel alternative spike-based antigen assay, comprising four high-affinity,...
Neutralizing antibodies represent a valuable therapeutic approach to countermeasure the current COVID-19 pandemic. Emergence of SARS-CoV-2 variants emphasizes notion that antibody treatments need rely on highly neutralizing monoclonal (mAbs), targeting several distinct epitopes for circumventing therapy escape mutants. Previously, we reported efficient human mAbs recognizing spike receptor-binding domain (RBD) SARS-CoV-2. Here report isolation, characterization, and recombinant production 12...
Abstract The eradication of smallpox was officially declared by the WHO in 1980, leading to discontinuation vaccination campaign against virus. Consequently, immunity and related orthopoxviruses like Monkeypox virus gradually declines, highlighting need for efficient countermeasures not only prevention, but also treatment already exposed individuals. We have recently developed human-like monoclonal antibodies (mAbs) from vaccinia virus-immunized non-human primates. Two mAbs, MV33 EV42,...
Ricin, a highly toxic plant-derived toxin, is considered potential weapon in biological warfare due to its high availability and ease of preparation. Pulmonary exposure ricin results the generation an acute edematous inflammation followed by respiratory insufficiency death. Passive immunization with polyclonal anti-ricin antibodies conferred protection against pulmonary ricinosis, however, at clinically-relevant time points for treatment, survival rates were limited. In this study,...
Ricin, derived from the castor bean plant Ricinus communis, is one of most potent and lethal toxins known, against which there no available antidote. To date, use neutralizing antibodies promising post-exposure treatment for ricin intoxication. The aim this study was to isolate high affinity anti-ricin that possess toxin-neutralization capabilities. Two non-human primates were immunized with either a ricin-holotoxin- or subunit-based vaccine, ensure elicitation diverse antibodies. By using...
Ricin, a plant-derived exotoxin, inhibits protein synthesis by ribosomal inactivation. Due to its wide availability and ease of preparation, ricin is considered biothreat, foremost respiratory exposure. We examined the in vivo interactions between cells lungs mice intranasally exposed toxin revealed multi-phasic cell-type-dependent binding profiles. While macrophages (MΦs) dendritic (DCs) displayed biphasic ricin, monophasic patterns were observed for other cell types; epithelial early...
The COVID-19 pandemic led to development of mRNA vaccines, which became a leading anti-SARS-CoV-2 immunization platform. Preclinical studies are limited infection-prone animals such as hamsters and monkeys in protective efficacy vaccines cannot be fully appreciated. We recently reported SARS-CoV-2 human Fc-conjugated receptor-binding domain (RBD-hFc) vaccine delivered via lipid nanoparticles (LNPs). BALB/c mice demonstrated specific immunologic responses following RBD-hFc vaccination. Now,...
Monoclonal antibodies represent an important avenue for COVID-19 therapy and are routinely used rapid accessible diagnosis of SARS-CoV-2 infection. The recent emergence genetic variants emphasized the need to enlarge repertoire that target diverse epitopes, combination which may improve immune-diagnostics, augment efficiency immunotherapy prevent selection escape-mutants. Antigen-specific controlled immunization experimental animals elicit antibody repertoires significantly differ from those...
This protocol describes the use of a biolayer interferometry platform for assessing antibody-antigen interactions. The focuses on affinity determination and epitope binning, although system can be utilized measuring any protein-protein interaction. Readings are collected in real time, allowing unlabeled molecules, data thus obtained fast easy manner. Experiments should carefully designed, taking into consideration tested interaction, available sensors, suitable controls. For complete details...
In this study, we assess an alternative Fc-fusion protein purification method that does not rely on chromatographic media or ligands. Recombinant human acetylcholinesterase, fused to the Fc domain of IgG1 (henceforth, AChE-Fc), was purified with precipitated aromatic complexes composed bathophenanthroline batho) chelator either Zn2+ Cu2+ ions (i.e., [(batho)3:Zn2+] [(batho)2:Cu2+]) in presence polyethylene glycol 6000 (PEG-6000). a three-step process conducted at pH 7, AChE-Fc captured by...
Background: Rift Valley fever virus (RVFV) is a zoonotic that poses significant threat to both livestock and human health has caused outbreaks in endemic regions. In humans, most patients experience febrile illness; however, some patients, RVF disease may result hemorrhagic fever, retinitis, or encephalitis. While several veterinary vaccines are being utilized countries, currently, there no licensed therapeutics for use. Neutralizing antibodies specifically targeting vulnerable pathogen...
Pharmaceutical companies are aware of the ongoing effort to satisfy increasing global demand for therapeutic grade monoclonal antibodies (mAbs), an especially difficult challenge poor and developing countries. We present a simple, economical, single-step purification approach at neutral pH polyclonal human IgG (hIgG) that does not require expensive ligands, chromatography columns, polymers, nor membranes. Instead, porous precipitates commercial, recyclable aromatic...
Ricin, a highly toxic glycoprotein derived from the seeds of Ricinus communis, poses significant risks in bioterrorism and toxicology due to its rapid absorption ease dissemination. Rapid, ultra-sensitive detection is crucial for timely medical intervention implementing security measures. However, existing methods often lack sufficient sensitivity or require lengthy processing, limiting their utility trigger-to-treat scenarios. Here, we present an optical modulation biosensing (OMB)-based...
Pharmaceutical companies are aware of the ongoing effort to satisfy increasing global demand for therapeutic-grade monoclonal antibodies (mAbs), an especially difficult challenge poor and developing countries. We present a simple, economical, single-step purification approach at neutral pH polyclonal human IgG (hIgG), which does not require any expensive ligands, chromatography columns, polymers, or membranes. Instead, porous precipitates commercial, recyclable aromatic...
Acetylcholinesterase (AChE) is the physiological target of organophosphate nerve agent compounds. Currently, development a formulation for prophylactic administration cholinesterases as bioscavengers in established risk situations exposure to agents incentive many efforts. While cholinesterase were found be highly effective conferring protection against animal models, their therapeutic use complicated by short circulatory residence time. To create bioscavenger with prolonged plasma...
ABSTRACT Ricin, one of the most potent and lethal toxins known, is classified by Centers for Disease Control Prevention (CDC) as a select agent. Currently, there no available antidote against ricin exposure, promising therapy based on neutralizing antibodies elicited active vaccination or that are given passively. The aim this study was to characterize repertoire anti-ricin generated in rabbits immunized with toxoid. These exhibit an exceptionally high avidity (thiocyanate-based index, 9 M)...
Glycosylation patterns commonly change in cancer, resulting expression of tumor-associated carbohydrate antigens (TACA). While promising, currently available anti-glycan antibodies are not useful for clinical cancer therapy. Here, we show that potent can be engineered to acquire therapeutic efficacy. We designed yeast surface display generate and select against the TACA SLea (CA19−9) colon pancreatic cancers. Elite clones showed increased affinity, better specificity, improved binding human...
A wide range of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing monoclonal antibodies (mAbs) have been reported, most which target the spike glycoprotein. Therapeutic implementation these has challenged by emerging SARS-CoV-2 variants harboring mutated versions. Consequently, re-assessment previously identified mAbs is high priority. Four selected targeting non-overlapping epitopes are now evaluated for binding potency to RBD versions, reported mediate escape from...
While potent monoclonal antibodies against ricin were introduced over the years, question whether increasing antibody affinity enables better toxin neutralization was not fully addressed yet. The aim of this study to characterize contribution potential antibody. cHD23 that targets B-subunit and interferes with its binding membranal receptors, isolated. In order create clones improved toward ricin, a scFv-phage display library containing mutated versions variable regions constructed...
Abstract Francisella tularensis ( Ft ), the causative agent of lethal tularemia, is classified as a category A biological warfare threat agent. While infection treatable by antibiotics, many failed antibiotic treatments were reported, highlighting need for effective new treatments. It has been demonstrated that binding antibody-coated bacteria to Fc receptor located on phagocytic cells key process needed efficient protection against . Yet, utilizes same enter in order escape immune system....
ABSTRACT Members of the Orthopoxvirus genus can cause severe infections in humans. Global vaccination against smallpox, caused by variola virus, resulted eradication disease 1980. Shortly thereafter, was discontinued, and as a result, large proportion current population is not protected orthopoxviruses. The concerns that virus or other engineered forms poxviruses may re-emerge bioweapons sporadic outbreaks zoonotic members family, such Mpox, which are becoming more frequent prevalent, also...