A5020107036- Biosensors and Analytical Detection
- Advanced Fluorescence Microscopy Techniques
- SARS-CoV-2 detection and testing
- Advanced Biosensing Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Electron Spin Resonance Studies
- Microfluidic and Bio-sensing Technologies
- Biotin and Related Studies
- Near-Field Optical Microscopy
- Force Microscopy Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Sensors
- Cell Image Analysis Techniques
- Click Chemistry and Applications
Ludwig-Maximilians-Universität München
2022-2025
TU Dortmund University
2024-2025
Abstract Single-molecule Förster-resonance energy transfer (smFRET) experiments allow the study of biomolecular structure and dynamics in vitro vivo. We performed an international blind involving 19 laboratories to assess uncertainty FRET for proteins with respect measured efficiency histograms, determination distances, detection quantification structural dynamics. Using two protein systems distinct conformational changes dynamics, we obtained ≤0.06, corresponding interdye distance precision...
Pulsed electron-electron double resonance spectroscopy (PELDOR/DEER) and single-molecule Förster energy transfer (smFRET) are frequently used to determine conformational changes, structural heterogeneity, inter probe distances in biological macromolecules. They provide qualitative information that facilitates mechanistic understanding of biochemical processes quantitative data for modelling. To a comprehensive comparison the accuracy PELDOR/DEER smFRET, we use library cysteine variants four...
Over the past decades, single-molecule and super-resolution microscopy have advanced represent essential tools for life science research. There is, however, a growing gap between state of art what is accessible to biologists, biochemists, medical researchers, or labs with financial constraints. To bridge this gap, we introduce Brick-MIC, versatile affordable open-source 3D-printed microspectroscopy imaging platform. Brick-MIC enables integration various fluorescence techniques resolution...
Abstract Attaching fluorescent dyes to biomolecules is essential for assays in biology, biochemistry, biophysics, biomedicine and imaging. A systematic approach the selection of suitable labeling sites macromolecules, particularly proteins, missing. We present a quantitative strategy identify such protein residues using naïve Bayes classifier. Analysis >100 proteins with ~400 successfully labeled allows four parameters, which can rank via single metric (the label score). The tested...
Over the past decades, single-molecule and super-resolution microscopy have advanced represent essential tools for life science research. There is,however, a growing gap between state-of-the-art what is accessible to biologists, biochemists, medical researchers or labs with financial constraints. To bridge this gap, we introduce Brick-MIC, versatile affordable open-source 3D-printed micro-spectroscopy imaging platform. Brick-MIC enables integration of various fluorescence techniques...
Abstract An essential requirement for the use of fluorescent dyes in biomedicine, molecular biology, biochemistry, biophysics and optical imaging is their (covalent) attachment to biomolecules. There is, however, no systematic automated approach selection suitable labeling sites macromolecules, which particular problematic proteins. Here, we present a general quantitative strategy identify optimal residues protein using naïve Bayes classifier. Based on literature search bioinformatics...
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Abstract Mainstream virus detection relies on the specific amplification of nucleic acids via polymerase chain reaction, a process that is slow and requires extensive laboratory expertise equipment. Other modalities, such as antigen-based tests, allow much faster but have reduced sensitivity. In this study, we report development flow virometer for rapid single nanoparticles based confocal microscopy. The combination laminar multiple dyes enable correlated fluorescence signals, providing...