A5102791520- Helicobacter pylori-related gastroenterology studies
- European and International Law Studies
- RNA modifications and cancer
- European Criminal Justice and Data Protection
- Cancer-related molecular mechanisms research
- Probiotics and Fermented Foods
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Taxation and Legal Issues
- International Law and Aviation
- Viral gastroenteritis research and epidemiology
- Bacterial Genetics and Biotechnology
- Human Rights and Immigration
- Genetic factors in colorectal cancer
- Healthcare Systems and Practices
- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Digitalization, Law, and Regulation
- Corporate Governance and Law
- Fermentation and Sensory Analysis
- Veterinary medicine and infectious diseases
- Galectins and Cancer Biology
- Carcinogens and Genotoxicity Assessment
- Disaster Management and Resilience
- Conflict of Laws and Jurisdiction
Institut Pasteur
2013-2025
Centre National de la Recherche Scientifique
1999-2025
Unité de Recherche Technologie et Analyses Laitières
2025
Université Paris Cité
2025
Université de Toulon
2013-2024
Roche (France)
2013-2024
University of Virginia
2023-2024
Université de Tours
2024
Actalia (France)
2014-2023
Engineering Systems (United States)
2023
Helicobacter pylori is a major cause of gastric carcinoma. To investigate possible link between bacterial infection and genetic instability the host genome, we examined effect H. on known cellular repair pathways in vitro vivo. Moreover, various types instabilities nuclear mitochondrial DNA (mtDNA) were examined.We observed effects cell line (AGS), C57BL/6 mice, individuals with chronic gastritis. In AGS cells, base excision mismatch (MMR) was analyzed by reverse transcription-PCR, Western...
H. pylori drug-resistant strains and non-compliance to therapy are the major causes of eradication failure. For some bacterial species it has been demonstrated that fatty acids have a growth inhibitory effect. Our main aim was assess ability docosahexaenoic acid (DHA) inhibit both in vitro mouse model. The effectiveness standard (ST) combination with DHA on recurrence prevention success also investigated. effects were analyzed an dose-response study n vivo We analized colonize mice gastric...
Helicobacter pylori (Hp) is a major risk factor for gastric cancer (GC). Hp promotes DNA damage and proteasomal degradation of p53, the guardian genome stability. reduces expression transcription USF1 shown to stabilise p53 in response genotoxic stress. We investigated whether Hp-mediated deregulation impacts p53-response consequently genetic instability. also explored vivo role carcinogenesis.Human epithelial cell lines were infected with Hp7.13, exposed or not DNA-damaging agent...
Metal acquisition is crucial for all cells and the virulence of many bacterial pathogens. In particular, nickel a determinant human gastric pathogen Helicobacter pylori as it cofactor two enzymes essential in vivo colonization, urease [NiFe] hydrogenase. To import despite its scarcity body, H. requires efficient uptake mechanisms that are only partially defined. Indeed, alternative ways entry were predicted to exist addition well-described NixA permease. Using genetic screen, we identified...
We are developing a genetic approach to study with single antibody the folding and topology of LamB, an integral outer membrane protein from Escherichia coli K-12. This consists inserting same reporter foreign antigenic determinant (the C3 epitope poliovirus) at different sites LamB so that resulting hybrid proteins have essentially kept in vivo biological properties therefore its cellular location structure; corresponding called permissive sites. A specific monoclonal can then be used...
Abstract Background: Helicobacter pylori infection is associated with gastric cancer. Study the Big Blue mouse model has reported a mutagenic effect H. infection, as result in part of oxidative DNA damage. The present work investigates consequences deficiency OGG1 glycosylase, responsible for excision 8‐oxo guanine, on inflammatory and genotoxic host response to infection. Materials Methods: Ogg1 –/– C57BL/6 mice were orally inoculated strain SS1 or vehicle only, sacrificed after 1, 3, 6...
Metal acquisition and intracellular trafficking are crucial for all cells metal ions have been recognized as virulence determinants in bacterial pathogens. Virulence of the human gastric pathogen Helicobacter pylori is dependent on nickel, cofactor two enzymes essential vivo colonization, urease [NiFe] hydrogenase. We found that small paralogous nickel-binding proteins with high content Histidine (Hpn Hpn-2) play a central role maintaining non-toxic nickel controlling its trafficking....
The psychrotrophic bacterium Pseudomonas fragi was subjected to cold shocks from 30 or 20 5 degrees C. downshifts were followed by a lag phase before growth resumed at characteristic C rate. analysis of protein patterns two-dimentional gel electrophoresis revealed overexpression 25 17 proteins and underexpression 12 following the 30- 20-to-5 shift, respectively. two shared similar variations synthesis proteins. kinetic distinguished induced into shock (Csps), which rapidly but transiently...
The multifactorial origin of gastric cancer encompasses environmental factors mainly associated with diet. Pteridium aquilinum-bracken fern-is the only higher plant known to cause in animals. Its carcinogenic toxin, ptaquiloside, has been identified milk cows and groundwater. Humans can be directly exposed by consumption plant, contaminated water or milk, spore inhalation. Epidemiological studies have shown an association between bracken exposure cancer. In present work, genotoxicity P....
Shiga toxin-producing Escherichia coli (STEC) is a food-borne pathogen that may be responsible for severe human infections. Only limited number of serotypes, including O26:H11, are involved in the majority serious cases and outbreaks. The main virulence factors, toxins (Stx), encoded by bacteriophages. Seventy-four STEC O26:H11 strains various origins (including human, dairy, cattle) were characterized their stx subtypes Stx phage chromosomal insertion sites. food cattle possessed stx(1a)...
Gastric cancer represents a major health burden worldwide and is often diagnosed at an advanced stage. Biomarkers for screening prevention of gastric are missing. Changes in peripheral blood mitochondrial DNA (mtDNA) have emerged as potential preventive/diagnosis biomarker risk. We aimed to determine whether leukocytes mtDNA levels associated with stages the carcinogenesis cascade.We measured by quantitative real-time PCR assay 28 patients non-atrophic gastritis (NAG), 74 cancer, 48 matched...
Shiga‐toxin producing Escherichia coli (STEC) strains may cause human infections ranging from simple diarrhea to Haemolytic Uremic Syndrome (HUS). The five main pathogenic serotypes of STEC (MPS‐STEC) identified thus far in Europe are O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28. Because can survive or grow during cheese making, particularly soft cheeses, a stochastic quantitative microbial risk assessment model was developed assess the HUS associated with MPS‐STEC raw milk cheeses. A...
Helicobacter pylori (Hp) infection is the main cause of peptic ulcer and gastric cancer. Hp eradication rates have fallen due to increasing bacterial resistance currently used broad-spectrum antimicrobials. We designed, synthesized, tested redox variants nitroethylene- 7-nitrobenzoxadiazole-based inhibitors essential protein flavodoxin. Derivatives 7-nitrobenzoxadiazole lead, carrying reduced forms nitro group and/or oxidized a sulfur atom, display high therapeutic indexes against several...
Gastric cancer is an inflammation-driven disease often associated with a bad prognosis. Upstream stimulatory factors USF1 and USF2 are pleiotropic transcription factors, tumor suppressor function. Low expression of low survival in gastric patients. genetic polymorphism -202G > A has been susceptibility. Our aim was to investigate gene serum level the risk cancer. USF1-202 G/A analyzed by sanger sequencing, measure USF1/USF2 levels ELISA H. pylori-positive patients chronic gastritis,...
Background: Gastric cancer (GC) is often associated with a poor prognosis, due to its asymptomatic early stages. The current gold standard for diagnosing GC, upper endoscopy, invasive and has limited sensitivity detecting gastric preneoplasia such as dysplasia. Non-invasive biomarkers, circulating proteins in the blood, hold promise detection of lesions. Methods: During an exploratory study, plasma samples from 39 participants, including patients diagnosed different pathologies (gastritis,...
ABSTRACT The performance of a new method developed in 2021 by the European Union Reference Laboratory (EURL) for Listeria monocytogenes based on 12 multiplex real-time PCR, allowing identification molecular serotype and 30 major L. multilocus sequence typing clonal complexes (CC), was assessed through interlaboratory validation trial (ILVT). This ILVT adapted from ISO standard 16140 part 6. Overall, 98 blinded pure strains ( monocytogene s or spp.), previously characterized EURL, were sent...
Raw cow milk microflora: diversity and influence of conditions production .The microbial composition 158 raw samples taken from 27 farms located in the French Alps area was determined.Microbial analyses included total mesophilic aerobic bacteria count as well numeration some useful cheesemaking microorganisms (lactic acid bacteria, halophilic propionic bacteria…), spoilage (Pseudomonas, enterobacteriaceae, Clostridium spp.) pathogenic (coagulase positive staphylococci, Escherichia coli).Milk...
Helicobacter pylori infection is associated with the development of gastric adenocarcinoma. Upstream stimulatory factors USF1 and USF2 regulate transcription genes related to immune response, cell cycle proliferation. A decrease in their expression observed human epithelial cells infected H. pylori, a lower binding DNA E-box recognition site as shown by electrophoretic mobility shift assay. methylation leads gene silencing. The treatment 5′-azacytidine, an inhibitor methylation, restored...
H. pylori colonizes half of the world's population leading to gastritis, ulcers and gastric cancer. strains resistant antibiotics are increasing which raises need for alternative therapeutic approaches. Docosahexaenoic acid (DHA) has been shown decrease growth its associated-inflammation through mechanisms poorly characterized. We aimed explore DHA action on pylori-mediated inflammation adhesion epithelial cells (AGS) also identify bacterial structures affected by DHA. metabolism was...
Targeting mitochondria is a powerful strategy for pathogens to subvert cell physiology and establish infection. Helicobacter pylori bacterial pathogen associated with gastric cancer development that known target directly exclusively through its pro-apoptotic vacuolating cytotoxin VacA. By in vitro infection of epithelial cells wild-type VacA-deficient H. strains, treatment purified VacA proteins mouse model, we show deregulates by two novel mechanisms, both rather host survival. First, early...