A5043808791- Helicobacter pylori-related gastroenterology studies
- Gastric Cancer Management and Outcomes
- Galectins and Cancer Biology
- Gut microbiota and health
- Veterinary medicine and infectious diseases
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Colorectal Cancer Screening and Detection
- Gastrointestinal disorders and treatments
- Mycobacterium research and diagnosis
- Eosinophilic Esophagitis
- Antibiotic Use and Resistance
- Genetic factors in colorectal cancer
- Peptidase Inhibition and Analysis
- Digestive system and related health
- Cancer Immunotherapy and Biomarkers
- Pancreatic and Hepatic Oncology Research
- Glycosylation and Glycoproteins Research
- Cancer-related molecular mechanisms research
- Esophageal Cancer Research and Treatment
- Radiomics and Machine Learning in Medical Imaging
- Advanced biosensing and bioanalysis techniques
- Wnt/β-catenin signaling in development and cancer
- Microbial Metabolites in Food Biotechnology
- Antioxidants, Aging, Portulaca oleracea
Universidade do Porto
2016-2025
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2016-2025
IPO Porto
2019-2023
Hospital Prof. Dr. Fernando Fonseca
2023
Algarve Biomedical Center
2019
University Medical Center Groningen
2016
Institute of Molecular Pathology and Pathomorphology
2012-2016
Gastric carcinoma development is triggered by Helicobacter pylori. Chronic H. pylori infection leads to reduced acid secretion, which may allow the growth of a different gastric bacterial community. This change in microbiome increase aggression mucosa and contribute malignancy. Our aim was evaluate composition microbiota chronic gastritis carcinoma.The retrospectively investigated 54 patients with 81 16S rRNA gene profiling, using next-generation sequencing. Differences microbial two patient...
The amount of host DNA, poses a major challenge to metagenome analysis. However, there is no guidance on the levels nor depth sequencing needed acquire meaningful information from whole (WMS). Here, we evaluated impact wide range amounts DNA and depths microbiome taxonomic profiling using WMS. Synthetic samples with increasing were created by spiking mock bacterial community, mouse-derived cell line. Taxonomic analysis revealed that proportions led decreased sensitivity in detecting very low...
There are no established predictive markers of progression gastric preneoplastic lesions. The aim this study was to analyze the relationship between Helicobacter pylori cagA and vacA genotypes lesions.This a follow-up that carried out in province Spain with high risk cancer. A total 312 patients who underwent upper endoscopy biopsy 1988-1994 diagnoses normal mucosa, non-atrophic gastritis (NAG), non-metaplastic multifocal atrophic (MAG), complete or incomplete intestinal metaplasia (IM),...
H. pylori drug-resistant strains and non-compliance to therapy are the major causes of eradication failure. For some bacterial species it has been demonstrated that fatty acids have a growth inhibitory effect. Our main aim was assess ability docosahexaenoic acid (DHA) inhibit both in vitro mouse model. The effectiveness standard (ST) combination with DHA on recurrence prevention success also investigated. effects were analyzed an dose-response study n vivo We analized colonize mice gastric...
Helicobacter pylori induces an invasive phenotype in gastric epithelial cells through a mechanism that requires the type IV secretion system and phosphorylation of c-Met. The E-cadherin-catenin complex is major component adherens junctions functions as invasion suppressor. We investigated whether E-cadherin has role H. pylori-induced, c-Met phosphorylation-dependent cell-invasive phenotype.AGS lack are to stimulation were transduced with infected pylori. NCI-N87 cells, which endogenously...
Summary Background First‐degree relatives ( FDR s) of early‐onset gastric carcinoma EOGC ) patients are at increased risk cancer development. OLGA / OLGIM (Operative Link on Gastritis/Intestinal Metaplasia Assessment) classifications have been proposed for the identification individuals high Aim To estimate prevalence and severity premalignant conditions lesions in s patients. Methods A case–control study was conducted encompassing 103 (cases) 101 age‐ gender‐matched controls, all submitted...
Ferreira R M, Machado J C, Leite Carneiro F & Figueiredo C (2012) Histopathology 60, 992–998 The number of Helicobacter pylori CagA EPIYA tyrosine phosphorylation motifs influences the pattern gastritis and development gastric carcinoma Aims: To characterize variation in virulence associated with Glu‐Pro‐Ile‐Tyr‐Ala (EPIYA) motifs, to explore its relationship histopathological features chronic carcinoma. Methods results: A total 169 H. ‐infected patients were studied. presence cagA type...
ABSTRACT Here, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as new diagnostic test for Helicobacter pylori clarithromycin resistance detection paraffin-embedded gastric biopsy specimens. Both retrospective study and prospective cohort were conducted to evaluate the specificity sensitivity of PNA-FISH determine H. resistance. In ( n = 30 patients), full agreement between PCR-sequencing was observed. Compared reference (culture...
The high background of host RNA poses a major challenge to metatranscriptome analysis human samples. Hence, metatranscriptomics has been mainly applied microbe-rich samples, while its application in tissues with low ratio microbial cells yet be explored. Since there is no computational workflow specifically designed for the taxonomic and functional this type we propose an effective strategy accurately characterize microbiome content. We experimentally generated synthetic samples...
The present report describes a novel method for genotyping the virulence-associated vacA intermediate (i) region of Helicobacter pylori in archive material. i-region genotypes as determined by were completely concordant with those sequence analysis and functional vacuolation activity. was further validated directly gastric biopsy specimens 386 H. pylori-positive cases, effective characterization i obtained 191 192 (99.5%) frozen 186 194 (95.9%) formalin-fixed paraffin-embedded specimens,...
Abstract Background Triple therapy is the gold standard treatment for Helicobacter pylori eradication from human stomach, but increased resistance to clarithromycin became main factor of failure. Until now, fastidious culturing methods are generally method choice assess status. In this study, a new genotypic detect in clinical samples, based on fluorescent situ hybridization (FISH) using set peptide nucleic acid probes (PNA), proposed. Results The targeting point mutations responsible was...
Heterogeneity at the Helicobacter pylori cagA gene promoter region has been linked to variation in CagA expression and gastric histopathology. Here, we characterized 46 H. strains from Portugal. Our results confirm relationship between protein originally observed Colombia. We that individuals with intestinal metaplasia were all infected containing a specific motif. Additionally, provided novel functional evidence strain-specific sequences levels influence interleukin 8 secretion by host...
Helicobacter pylori colonizes the human stomach and increases risk for peptic ulcer disease gastric carcinoma. H. upregulates expression activity of several matrix metalloproteinases (MMPs) in cell lines mucosa. The aim this study was to explore mechanisms leading upregulation MMP10 epithelial cells induced by Infection with led an increase levels MMP-10 messenger RNA, protein secretion, activity. cagA knockout mutants or CagA phosphorylation-defective failed expression. These results were...
Abstract Emerging evidence of the relationship between microbiome composition and development numerous diseases, including cancer, has led to an increasing interest in study human microbiome. Technological breakthroughs regarding DNA sequencing methods propelled studies with a large number samples, which called for necessity more sophisticated data-analytical tools analyze this complex relationship. The aim work was develop machine learning-based approach distinguish type cancer based on...
Abstract Introduction Gastric cancer (GC) burden is currently evolving with regional differences associated complex behavioural, environmental, and genetic risk factors. The LEGACy study a Horizon 2020-funded multi-institutional research project conducted prospectively to provide comprehensive data on the tumour biological characteristics of gastroesophageal from European LATAM countries. Material methods Treatment-naïve advanced adenocarcinoma patients were recruited in seven Formalin-fixed...
Abstract Gastric cancer (GC) has a poor prognosis. The LEGACy consortium been established to enhance GC outcomes though improved primary and secondary prevention strategies. We performed an educational intervention study using online module disseminate knowledge about risk factors symptoms the general population. Participants were recruited through various media channels exposed questionnaire assess their knowledge, before after intervention. included informative brochure short video...
Gastric cancer (GC) is recognized for intrinsic heterogeneity, although it similarly approached in Europe and Latin America (LATAM). The LEGACY project aimed to deepen GC molecular understanding through multi-omics analysis LATAM samples. Tumor samples were centrally reviewed histology, human epidermal growth factor receptor 2 (HER2) expression, mismatch repair-deficient (dMMR)/microsatellite instability (MSI) status. In addition, we assessed Epstein-Barr virus (EBV) status, programmed...
Abstract Background Gastric cancer (GC) patients from European (EU) and especially Latin American (LATAM) countries are underrepresented in previous large-scale multi-omic studies that have identified clinically relevant subgroups. The LEGACY study aimed to profile the molecular immunological features of GCs EU LATAM countries. Methods Tumor biopsies 95 56 were profiled with immunohistochemistry (CD3, CD8, FOXP3, PD-L1, MSI HER2), Nanostring mRNA expression analyses, microbiome sequencing....