A5015698166- Glycosylation and Glycoproteins Research
- Helicobacter pylori-related gastroenterology studies
- Digestive system and related health
- Galectins and Cancer Biology
- Genetic factors in colorectal cancer
- Monoclonal and Polyclonal Antibodies Research
- Gastric Cancer Management and Outcomes
- Carbohydrate Chemistry and Synthesis
- Gastrointestinal disorders and treatments
- Eosinophilic Esophagitis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- Gastrointestinal Tumor Research and Treatment
- Metastasis and carcinoma case studies
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Proteoglycans and glycosaminoglycans research
- Cancer-related gene regulation
- Cell Adhesion Molecules Research
- Peptidase Inhibition and Analysis
- Infective Endocarditis Diagnosis and Management
- Protease and Inhibitor Mechanisms
- Cervical Cancer and HPV Research
Universidade do Porto
2015-2024
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2024
University of Port Harcourt
2019-2024
Hôpital Saint Joseph
2018-2023
Université Paris Cité
2020
Infection, Anti-microbiens, Modélisation, Evolution
2020
University of Trieste
2017
Portuguese League Against Epilepsy
2017
University of Alberta
2015-2016
Institute of Molecular Pathology and Pathomorphology
2004-2014
Abstract Intestinal metaplasia (IM) is part of a stepwise sequence alterations the gastric mucosa, leading ultimately to cancer, and strongly associated with chronic Helicobacter pylori infection. The molecular mechanisms underlying onset IM remain elusive. aim this study was assess putative involvement two intestine‐specific transcription factors, CDX1 CDX2, in pathogenesis carcinoma. Eighteen foci 46 cases carcinoma were evaluated by immunohistochemistry for CDX2 expression. expressed all...
BLAST analysis of expressed sequence tags (ESTs) using the coding human UDP-galactose:beta-N-acetylglucosamine beta1, 4-galactosyltransferase, designated beta4Gal-T1, revealed a large number ESTs with identical as well similar sequences. sequences to that beta4Gal-T1 could be grouped into at least two non-identical sets. Analysis predicted amino acid novel conservation short motifs cysteine residues previously shown important for function beta4Gal-T1. The likelihood identified represented...
In order to investigate the expression of MUC5AC mucin in normal gastric mucosa and carcinomas, we produced 3 monoclonal antibodies (MAbs) using a synthetic peptide. The immunohistochemical study was performed one these MAbs (CLH2) which reacted with different designs peptides based on tandem repeat native deglycosylated extracted from tissues. CLH2 immunoreactivity restricted foveolar mucopeptic neck cells mucosa. No reactivity observed type-I intestinal metaplasia. Out 66 42 (63.6%)...
Abstract Mucins are a large family of heavily O-glycosylated proteins that cover all mucosal surfaces and constitute the major macromolecules in most body fluids. primarily defined by their variable tandem repeat (TR) domains densely decorated with different O-glycan structures distinct patterns, these arguably convey much informational content mucins. Here, we develop cell-based platform for display production human TR O-glycodomains (~200 amino acids) tunable patterns O-glycans using...
To investigate the expression of MUC6 mucin in gastric carcinomas, we generated a novel monoclonal antibody (MAb CLH5) using an synthetic peptide. MAb CLH5 reacted exclusively with peptide and native deglycosylated extracts from tissues. immunoreactivity was observed normal mucosa restricted to pyloric glands antrum mucopeptic cells neck zone body region. In series 104 31 (29.8%) were immunoreactive for MUC6. The not associated histomorphological type or clinicopathological features...
In intestinal metaplasia and 30% of gastric carcinomas, MUC2 mucin the intestine-specific transcription factors Cdx-1 Cdx-2 are aberrantly expressed. The involvement in development their role several genes support hypothesis that and/or play important roles aberrant differentiation program carcinoma. To clarify mechanisms transcriptional regulation gene cells, pGL3 deletion constructs covering 2.6 kb human promoter were used transient transfection assays, enabling us to identify a relevant...
Chronic Helicobacter pylori infection is recognized as a cause of gastric cancer. H. adhesion to cells mediated by bacterial adhesins such sialic acid–binding adhesin (SabA), which binds the carbohydrate structure sialyl–Lewis x. Sialyl–Lewis x expression in epithelium induced during persistent infection, suggesting that modulates host cell glycosylation patterns for enhanced adhesion. Here, we evaluate changes glycosylation-related gene profile human carcinoma line following infection. We...
Glycoconjugates expressed on gastric mucosa play a crucial role in host-pathogen interactions. The FUT2 enzyme catalyzes the addition of terminal alpha(1,2)fucose residues, producing H type 1 structure surface epithelial cells and mucosal secretions secretor individuals. Inactivating mutations human gene are associated with reduced susceptibility to Helicobacter pylori infection. H. infects over half world's population causes diverse lesions, from gastritis cancer. adhesion constitutes step...
Changes in mucin protein expression and glycosylation are common features pre-neoplastic lesions cancer therefore used as cancer-associated markers. De novo of intestinal MUC2 sialyl-Tn antigen frequently observed metaplasia (IM) gastric cancer. However, despite that these antigens often co-localize, has not been demonstrated to be a carrier sialyl-Tn. By using the situ proximity ligation assay (in PLA), we herein could show is major all IM cases most carcinoma cases. The requirement by PLA...
The extracellular matrix (ECM) plays critical roles in tumor progression and metastasis. However, the contribution of ECM proteins to early metastatic onset peritoneal cavity remains unexplored. Here, we suggest a new route metastasis through interaction integrin alpha 2 (ITGA2) with collagens enriched coinciding poor outcome patients ovarian cancer. Using multiple gene-edited cell lines patient-derived samples, demonstrate that ITGA2 triggers cancer adhesion collagen, promotes migration,...
The membrane-bound MUC1 mucin is expressed in normal mucosas and the aberrant expression of its under-glycosylated forms has been reported carcinomas from different sites. Several studies have provided conflicting evidence regarding relationship between outcome cancer patients. In this study, we investigated immunohistochemical epitopes, using 2 monoclonal antibodies (MAbs): HMFG1, which reacts with fully glycosylated MUC1, was studied 73 gastric carcinomas; SM3, recognises an form 180...
Mutations in recently identified genes on chromosomes 2 and 3 seem to be responsible for repair errors (RER+) throughout the genome. This novel genetic mechanism was first reported hereditary non-polyposis colorectal cancer syndrome cancers that are characteristic of this syndrome, such as carcinomas right colon, stomach endometrium. We investigated frequency RER+ phenotype a series 34 sporadic gastric carcinomas, an attempt see if cases displayed any particular morphologic features and/or...
Salivary duct carcinoma is a relatively uncommon aggressive neoplasm, typically found in the parotid glands of older men. The histologic appearance that an situ and invasive high-grade adenocarcinoma, it closely resembles ductal breast. Several variants latter are very well known, but only papillary, sarcomatoid, low-grade subtypes have so far been reported salivary carcinoma. This study describes clinicopathologic immunohistochemical findings four examples additional previously undescribed...
Abstract Helicobacter pylori (Hp) infects half the world's population and causes diverse gastric lesions, from gastritis to cancer. Our aim was evaluate significance of secretor Lewis status in infection vitro adherence by Hp expressing BabA adhesin. We enrolled 304 Hp‐infected individuals Northern Portugal. Gastric biopsies, blood saliva were collected. Polymerase chain reaction (PCR) immunofluorescence used detect + biopsies. In a strain biopsies performed. Secretor identified Ulex, lectin...
Mucin glycoproteins are major secreted or membrane-bound molecules that, in cancer, show modifications both the mucin proteins expression and O-glycosylation profile, generating some of most relevant tumour markers clinical use for decades. Thus far, identification these biomarkers has been based on detection either protein O-glycan modifications. We therefore aimed to identify combined features, that is, specific glycoforms, an attempt increase specificity cancer biomarkers. Using situ...
Gastric cancer is preceded by a carcinogenesis pathway that includes gastritis caused Helicobacter pylori infection, chronic atrophic may progress to intestinal metaplasia (IM), dysplasia, and ultimately gastric carcinoma of the more common subtype. The identification glycosylation changes in circulating serum proteins patients with precursor lesions high interest represents source putative new biomarkers for early diagnosis intervention. This study applies glycoproteomic approach identify...
Helicobacter pylori infection is the main risk factor for intestinal metaplasia (IM) and gastric cancer development. IM a pre-neoplastic lesion, induced by transcription CDX2, where mucosa converted to an phenotype. We previously demonstrated that key elements of bone morphogenetic protein (BMP) pathway co-localize with CDX2 in upregulate expression cell lines. These observations, together hypothesis could be repressed SOX2, led us test whether H. , through BMPs, SOX2 participate molecular...