A5051598334- Digestive system and related health
- Prion Diseases and Protein Misfolding
- Genetic factors in colorectal cancer
- Fermentation and Sensory Analysis
- Glycosylation and Glycoproteins Research
- Carcinogens and Genotoxicity Assessment
- Helicobacter pylori-related gastroenterology studies
- Cancer-related gene regulation
- Reproductive System and Pregnancy
- Carbohydrate Chemistry and Synthesis
- Horticultural and Viticultural Research
- Neurological diseases and metabolism
- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
- Cytokine Signaling Pathways and Interactions
- Plant biochemistry and biosynthesis
- RNA modifications and cancer
- Parasitic Infections and Diagnostics
- Coccidia and coccidiosis research
- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Cancer Cells and Metastasis
- Trace Elements in Health
- Genomic variations and chromosomal abnormalities
- Polyamine Metabolism and Applications
Universidade do Porto
2003-2025
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2018-2025
Universidade Federal do Amazonas
2025
Sabin Medicina Diagnostica (Brazil)
2019-2024
Instituto Nacional de Investigação Agrária e Veterinária
2012-2020
University of Lisbon
1996-2018
Universidade Federal de Santa Catarina
2013-2014
Institute of Molecular Pathology and Pathomorphology
2004
Universidade Nova de Lisboa
1996-2001
Polytechnic Institute of Castelo Branco
2001
In intestinal metaplasia and 30% of gastric carcinomas, MUC2 mucin the intestine-specific transcription factors Cdx-1 Cdx-2 are aberrantly expressed. The involvement in development their role several genes support hypothesis that and/or play important roles aberrant differentiation program carcinoma. To clarify mechanisms transcriptional regulation gene cells, pGL3 deletion constructs covering 2.6 kb human promoter were used transient transfection assays, enabling us to identify a relevant...
Gastric cancer remains a serious health burden with few therapeutic options. Therefore, the recognition of stem cells (CSCs) as seeds tumorigenic process makes them prime target. Knowing that transcription factors SOX2 and OCT4 promote stemness, our approach was to isolate stem-like in human gastric cell lines using traceable reporter system based on SOX2/OCT4 activity (SORE6-GFP). Cells transduced SORE6-GFP were sorted into SORE6+ SORE6- populations, their biological behavior characterized....
Intestinal metaplasia (IM) is a preneoplastic lesion of the stomach in which there transdifferentiation gastric mucosa to an intestinal phenotype. The caudal-related homeobox gene CDX2 encodes intestine-specific transcription factor crucial for regulation proliferation and differentiation cells. In addition, involved induction IM stomach. aim this study was access putative involvement OCT-1 expression de novo leading onset IM. protein evaluated by immunohistochemistry 31 biopsies with...
Abstract Background Gastric cancer (GC) is the fourth most common cause of cancer-related mortality and fifth worldwide. Despite efforts, identification biomarkers new therapeutic approaches for GC remains elusive. Recent studies have begun to reveal role N6-adenosine methylation (m 6 A) in regulation gene expression. Methods The expression reader YT521-B homology domain-containing family 3 (YTHDF3) was assessed 331 patients using immunohistochemistry. cell lines depleted YTHDF3 CRISPR-Cas9...
In the present work, single grape variety wines, Moscatel and Arinto, were used. Analysis by denaturing polyacrylamide gel electrophoresis of wine proteins revealed presence only a few polypeptides ranging in molecular mass from 15 to 30 kDa. However, more detailed examination whole protein fraction, combination techniques, showed that these wines contain very large number (many tens and, possibly, many more) distinct polypeptides, exhibiting similar masses but different electrical charges....
Six Portuguese varietal wines (Fernão Pires, Assario, Tamarez, Verdelho, Arinto, and Moscatel) were used to study the factors that control protein insolubility in stored at high temperatures. Each wine exhibited a characteristic pattern of turbidity formation when exposed increasing temperatures (30 80 °C). This formation, highly specific for each wine, also varied prepared from same grape variety different vintages. was not determined by proteins. When A depleted its own back-added with B,...
Gastric cancer is one of the most frequent tumours and third leading cause cancer-related death worldwide. The investigation new biomarkers that can predict patient outcome more accurately allow better treatment follow-up decisions crucial importance. SOX9 (sex-determining region Y (SRY)-box 9) a regulator cell fate in embryogenesis adulthood. Here, we sought to ascertain relevance transcription factor as prognostic marker gastric cancer. expression was analyzed by immunohistochemistry 333...
Abstract Background Colorectal cancer (CRC) remains a serious health concern worldwide. Despite advances in diagnosis and treatment, about 15 to 30% of stage II CRC patients subjected tumor resection with curative intent, develop disease relapse. Moreover, the therapeutic strategy adopted after surgery is not consensual for these patients. This supports imperative need find new prognostic predictive biomarkers CRC. Methods For this purpose, we used one-hospital series 227 patient samples...
In this study we describe the development of strain BMX100, a new Escherichia coli K12 tester strain, derived from MX100, which was constructed for detection mutagens and mechanistic studies chemical carcinogens. We demonstrate here that BMX100 can be used stable expression human CYP1A2 or fused to rat liver NADPH cytochrome P450 reductase. Mutagenicity precarcinogens known bioactivated by CYP1A2, namely 2-aminoanthracene (2-AA), aflatoxin Bl (AFB1) 2-amino-3-methylimidazo[4,5-f]quinoline...
Aberrant glycosylation of mucins is a common phenomenon associated with oncogenic transformation. We investigated the association between expression tumor-associated antigens T, Tn, and sialyl-Tn polymorphism in length MUC1 mucin tandem repeat series gastric carcinomas. further evaluated relevance on these carbohydrate (TACAs) using carcinoma cell line model expressing recombinant constructs carrying 0, 3, 9, 42 repeats. Gastric carcinomas showed high prevalence Tn antigens, whereas T...
Aging | doi:10.18632/aging.203074. Taciane Barbosa Henriques, Diandra Zipinotti dos Santos, Isabella Santos Guimarães, Nayara Gusmão Tessarollo, Paulo Cilas Morais Lyra-Junior, Patricia Mesquita, Diana Pádua, Ana Luisa Amaral, Bruno Cavadas, Pereira, Ian Victor Silva, Raquel Maria da Silva Graça Almeida, Leticia Batista Azevedo Rangel
The establishment of an association between prion protein gene (PRNP) polymorphisms and scrapie susceptibility in sheep has enabled the development breeding programmes to increase resistance European Union. Intense selection for PRNP genotype may lead correlated genes linked PRNP. We intended investigate if any exists genetic variation prion-like Doppel (PRND) susceptibility, determined through genotyping. Sampling included 460 from eight Portuguese breeds PRND coding region was analysed by...
A hallmark of prion diseases or transmissible spongiform encephalopaties is the conversion cellular protein (PrPC), expressed by gene (prnp), into an abnormally folded isoform (PrPSc) with amyloid-like features that causes scrapie in sheep among other diseases. prnp together prnd (which encodes a prion-like designated as Doppel), and prnt (that testis specific - Prt) sprn (shadow gene, Shadoo Sho) genes, constitute "prion complex". Whereas role for proper functioning male reproductive system...
Summary The objective of this study was to search for polymorphisms in the ovine prion‐related protein (testis‐specific) gene ( PRNT ). Sampling included 567 sheep from eight Portuguese breeds. gene‐coding region analyzed by single‐strand conformation polymorphism and sequencing, allowing identification first polymorphisms, codons 6, 38, 43 48: c.17C>T (p.Ser6Phe, which disrupts a consensus arginine‐X‐X‐serine/threonine motif); c.112G>C (p.Gly38>Arg); c.129T>C c.144A>G...
Gastric cancer is a serious health problem worldwide. Although its incidence decreasing, the five-year survival rate remains low. Thus, it essential to identify new biomarkers that could promote better diagnosis and treatment of patients with gastric cancer. High-mobility group AT-hook 1 (HMGA1) non-histone, chromatin-binding protein has been found overexpressed in several tumor types. It correlated invasion, metastasis, drug resistance, leading worse patient survival. The aim this work was...