A5005629871- Digestive system and related health
- Helicobacter pylori-related gastroenterology studies
- Gastric Cancer Management and Outcomes
- Genetic factors in colorectal cancer
- Gastrointestinal disorders and treatments
- Glycosylation and Glycoproteins Research
- Esophageal Cancer Research and Treatment
- Colorectal Cancer Screening and Detection
- Metastasis and carcinoma case studies
- Cancer Cells and Metastasis
- Eosinophilic Esophagitis
- Lung Cancer Treatments and Mutations
- Colorectal Cancer Treatments and Studies
- Skin and Cellular Biology Research
- Cancer-related gene regulation
- Galectins and Cancer Biology
- Cancer Immunotherapy and Biomarkers
- Hair Growth and Disorders
- Veterinary Oncology Research
- Seaweed-derived Bioactive Compounds
- Algal biology and biofuel production
- Wnt/β-catenin signaling in development and cancer
- Esophageal and GI Pathology
- Cytokine Signaling Pathways and Interactions
- Peptidase Inhibition and Analysis
Universidade do Porto
2015-2025
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2025
St Thomas' Hospital
2025
IPO Porto
2023-2024
Hospital de São João
2024
Hospital de Santa Maria
2022
Institute of Molecular Pathology and Pathomorphology
2012
Universidad Complutense de Madrid
2012
Main Recommendations Patients with chronic atrophic gastritis or intestinal metaplasia (IM) are at risk for gastric adenocarcinoma. This underscores the importance of diagnosis and stratification these patients. High definition endoscopy chromoendoscopy (CE) is better than high white-light alone this purpose. Virtual CE can guide biopsies staging metaplastic changes target neoplastic lesions. Biopsies should be taken from least two topographic sites (antrum corpus) labelled in separate...
The homeobox transcription factor CDX2 plays a crucial role in intestinal cell fate specification, both during normal development and tumorigenic processes involving reprogramming. regulatory network is intricate, but it has not yet been fully uncovered. Through genome-wide screening of 3D culture system, the RNA-binding protein MEX3A was identified as putatively involved regulation; therefore, its biological relevance addressed by setting up cell-based assays together with expression...
Understanding the determinants of Helicobacter pylori infection in adults is essential to predict burden H. pylori-related diseases. We aimed estimate prevalence and incidence identify its major sociodemographic correlates an urban population from North Portugal.A representative sample noninstitutionalized adult inhabitants Porto (n = 2067) was evaluated by ELISA (IgG) a subsample 412) tested Western Blot assess with CagA-positive strains. Modified Poisson regression models were used crude...
Proteomics is a powerful approach to study the molecular mechanisms of cancer. In this study, we aim characterize proteomic profile gastric cancer (GC) in patients with diabetes mellitus (DM) type 2. Forty GC tissue samples including 19 cases from diabetic and 21 individuals without (control group) were selected for proteomics analysis. Gastric tissues processed following single-pot, solid-phase-enhanced sample preparation approach—SP3 enzymatic digestion trypsin. The resulting peptides...
Helicobacter pylori infection is the main risk factor for intestinal metaplasia (IM) and gastric cancer development. IM a pre-neoplastic lesion, induced by transcription CDX2, where mucosa converted to an phenotype. We previously demonstrated that key elements of bone morphogenetic protein (BMP) pathway co-localize with CDX2 in upregulate expression cell lines. These observations, together hypothesis could be repressed SOX2, led us test whether H. , through BMPs, SOX2 participate molecular...
We aimed to refine the value of CDX 2 as an independent prognostic and predictive biomarker in colorectal cancer ( CRC ) according disease stage chemotherapy sensitivity preclinical models. expression was evaluated 1045 I– IV primary s by gene n = 403) or immunohistochemistry 642) relation 5‐year relapse‐free survival RFS ), overall OS chemotherapy. Pharmacogenomic associations between 69 chemotherapeutics were assessed drug screening 35 cell lines. lost 11.6% cases showed poor multivariable...
Gastric cancer remains a serious health burden with few therapeutic options. Therefore, the recognition of stem cells (CSCs) as seeds tumorigenic process makes them prime target. Knowing that transcription factors SOX2 and OCT4 promote stemness, our approach was to isolate stem-like in human gastric cell lines using traceable reporter system based on SOX2/OCT4 activity (SORE6-GFP). Cells transduced SORE6-GFP were sorted into SORE6+ SORE6- populations, their biological behavior characterized....
Helicobacter pylori infection induces intestinal metaplasia of the stomach, a preneoplastic lesion associated with an increased risk for gastric cancer development. Intestinal is induced by intestine-specific transcription factor CDX2 but mechanisms responsible this ectopic expression have never been described. We hypothesized that BMP/SMAD pathway has role in regulation, context, following reasons: (1) BMP crucial normal differentiation and (2) there influx BMP2 BMP4-producing cells to...
<h3>Background and aims</h3> Intestinal metaplasia (IM) is a gastric preneoplastic lesion that appears following <i>Helicobacter pylori</i> infection confers an increased risk for development of cancer. It induced by expression the intestine-specific transcription factor CDX2. The regulatory mechanisms involved in triggering maintaining CDX2 have not been fully elucidated. Cdx2<sup>+/−</sup> mouse develops intestinal polyps with differentiation total loss Cdx2 absence structural second...
Gastric cancer remains a serious health concern worldwide. Patients would greatly benefit from the discovery of new biomarkers that predict outcome more accurately and allow better treatment follow-up decisions. Here, we used retrospective, observational study to assess expression prognostic value transcription factors SOX2 CDX2 in gastric cancer. SOX2, CDX2, MUC5AC MUC2 were assessed 201 tumors by immunohistochemistry. crossed with clinicopathological data determine their impact on tumor...
Abstract Introduction Gastric cancer (GC) is one of the most lethal malignancies worldwide. Helicobacter pylori primary cause GC; therefore, its eradication reduces risk developing this neoplasia. There extensive evidence regarding quadruple therapy with relevance to European population. However, in Latin America, data are scarce. Furthermore, there limited information about rates achieved by antibiotic schemes and American populations. Objective To compare effectiveness standard triple...
The tumor microenvironment encompasses several stressful conditions for cancer cells such as hypoxia, oxidative stress and pH alterations. Galectin-3, a well-studied member of the beta-galactoside-binding animal family lectins has been implicated in multiple steps metastasis cell-cell cell-ECM adhesion, promotion angiogenesis, cell proliferation resistance to apoptosis. However, both its aberrantly up- down-regulated expression was observed types cancer. Thus, mechanisms that regulate...
Intestinal metaplasia (IM), which results from de-novo expression of CDX2, and dysplasia are precursor lesions gastric cancer that associated with an increased risk for development. There is much evidence suggesting a role the transcription factor SOX2 in differentiation. The aim this study was to attempt establish relationship CDX2 differentiation reprogramming characterizes carcinogenesis, assess their involvement IM dysplasia.Characterization (SOX2, MUC5AC, MUC6) intestinal (CDX2 MUC2)...
Abstract Introduction Gastric cancer (GC) burden is currently evolving with regional differences associated complex behavioural, environmental, and genetic risk factors. The LEGACy study a Horizon 2020-funded multi-institutional research project conducted prospectively to provide comprehensive data on the tumour biological characteristics of gastroesophageal from European LATAM countries. Material methods Treatment-naïve advanced adenocarcinoma patients were recruited in seven Formalin-fixed...
Background Pembrolizumab monotherapy is an established front-line treatment for advanced non-small cell lung cancer (NSCLC) with programmed death-ligand 1 (PD-L1) tumor proportion score (TPS)≥50%. However, real-world data on its long-term efficacy remains sparse. Methods This study assessed 5-year outcomes of first-line pembrolizumab in a large, multicenter, cohort patients NSCLC and PD-L1 TPS≥50%, referred to as Pembro-real 5Y. Individual patient-level (IPD) from the experimental arm...
Gastric cancer (GC) is recognized for intrinsic heterogeneity, although it similarly approached in Europe and Latin America (LATAM). The LEGACY project aimed to deepen GC molecular understanding through multi-omics analysis LATAM samples. Tumor samples were centrally reviewed histology, human epidermal growth factor receptor 2 (HER2) expression, mismatch repair-deficient (dMMR)/microsatellite instability (MSI) status. In addition, we assessed Epstein-Barr virus (EBV) status, programmed...
Abstract Background Gastric cancer (GC) patients from European (EU) and especially Latin American (LATAM) countries are underrepresented in previous large-scale multi-omic studies that have identified clinically relevant subgroups. The LEGACY study aimed to profile the molecular immunological features of GCs EU LATAM countries. Methods Tumor biopsies 95 56 were profiled with immunohistochemistry (CD3, CD8, FOXP3, PD-L1, MSI HER2), Nanostring mRNA expression analyses, microbiome sequencing....
Abstract Gastric cancer (GC) has a poor prognosis. The LEGACy consortium been established to enhance GC outcomes though improved primary and secondary prevention strategies. We performed an educational intervention study using online module disseminate knowledge about risk factors symptoms the general population. Participants were recruited through various media channels exposed questionnaire assess their knowledge, before after intervention. included informative brochure short video...
O artigo tem como finalidade relatar o caso de um paciente que apresentou aumento súbito da pressão arterial, associado a alteração renal com evolução para insuficiência rapidamente progressiva necessidade hemodiálise, Acidente Vascular Encefálico (AVE) hemorrágico intraparenquimatoso e alterações hemorrágicas na retina. Foi atendido tratado no Hospital Regional Sobradinho - DF, evoluindo alta médica vaga diálise. Desse modo, importância do relato discussão hipertensão maligna, se destaca...
Gastric cancer is one of the most frequent tumours and third leading cause cancer-related death worldwide. The investigation new biomarkers that can predict patient outcome more accurately allow better treatment follow-up decisions crucial importance. SOX9 (sex-determining region Y (SRY)-box 9) a regulator cell fate in embryogenesis adulthood. Here, we sought to ascertain relevance transcription factor as prognostic marker gastric cancer. expression was analyzed by immunohistochemistry 333...
Abstract Background Colorectal cancer (CRC) remains a serious health concern worldwide. Despite advances in diagnosis and treatment, about 15 to 30% of stage II CRC patients subjected tumor resection with curative intent, develop disease relapse. Moreover, the therapeutic strategy adopted after surgery is not consensual for these patients. This supports imperative need find new prognostic predictive biomarkers CRC. Methods For this purpose, we used one-hospital series 227 patient samples...