A5016034491- Digestive system and related health
- Helicobacter pylori-related gastroenterology studies
- Glycosylation and Glycoproteins Research
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
- Carbohydrate Chemistry and Synthesis
- Gastric Cancer Management and Outcomes
- Galectins and Cancer Biology
- Gastrointestinal disorders and treatments
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Proteoglycans and glycosaminoglycans research
- Ovarian cancer diagnosis and treatment
- Cancer-related gene regulation
- Eosinophilic Esophagitis
- Cultural, Media, and Literary Studies
- Metastasis and carcinoma case studies
- Esophageal and GI Pathology
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Coccidia and coccidiosis research
- Mycobacterium research and diagnosis
- Cytokine Signaling Pathways and Interactions
- Hedgehog Signaling Pathway Studies
- RNA Research and Splicing
Universidade do Porto
2015-2025
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2016-2025
Cooperativa de Ensino Superior Politécnico e Universitário
2024
University of Aveiro
2023
Rede de Química e Tecnologia
2023
Hospital Beatriz Ângelo
2023
Bharati Vidyapeeth Deemed University
2022
IPO Porto
2009-2021
National Statistical Institute of Portugal
2018
Institute of Molecular Pathology and Pathomorphology
2004-2012
Abstract Intestinal metaplasia (IM) is part of a stepwise sequence alterations the gastric mucosa, leading ultimately to cancer, and strongly associated with chronic Helicobacter pylori infection. The molecular mechanisms underlying onset IM remain elusive. aim this study was assess putative involvement two intestine‐specific transcription factors, CDX1 CDX2, in pathogenesis carcinoma. Eighteen foci 46 cases carcinoma were evaluated by immunohistochemistry for CDX2 expression. expressed all...
Abstract The Sialyl-Tn antigen (Neu5Acα2–6GalNAc-O-Ser/Thr) is highly expressed in several human carcinomas and associated with carcinoma aggressiveness poor prognosis. We characterized two sialyltransferases, CMP-Neu5Ac:GalNAc-R α2,6-sialyltransferase (ST6GalNAc)-I ST6GalNAc-II, that are candidate enzymes for synthases. soluble recombinant hST6GalNAc-I hST6GalNAc-II the substrate specificity of both toward a panel glycopeptides, glycoproteins, other synthetic glycoconjugates. ST6GalNAc-I...
A seventh member of the human β4-galactosyltransferase family, β4Gal-T7, was identified by BLAST analysis expressed sequence tags. The coding region β4Gal-T7 depicts a type II transmembrane protein with similarity to β4-galactosyltransferases, but distinct in known motifs and did not contain cysteine residues conserved other six members β4Gal-T family. genomic organization different from previous β4Gal-Ts. Expression insect cells showed that gene product had β1,4-galactosyltransferase...
BLAST analysis of expressed sequence tags (ESTs) using the coding human UDP-galactose:beta-N-acetylglucosamine beta1, 4-galactosyltransferase, designated beta4Gal-T1, revealed a large number ESTs with identical as well similar sequences. sequences to that beta4Gal-T1 could be grouped into at least two non-identical sets. Analysis predicted amino acid novel conservation short motifs cysteine residues previously shown important for function beta4Gal-T1. The likelihood identified represented...
BLAST analysis of expressed sequence tags (ESTs) using the coding a human UDP-galactose:β-<i>N</i>-acetyl-glucosamine β-1,3-galactosyltransferase, designated β3Gal-T1, revealed no ESTs with identical sequences but large number similarity. Three different sets overlapping similarities to β3Gal-T1 were compiled, and complete regions these genes obtained. Expression two in Baculo virus system showed that one represented β-1,3-galactosyltransferase (β3Gal-T2) similar kinetic properties as...
In intestinal metaplasia and 30% of gastric carcinomas, MUC2 mucin the intestine-specific transcription factors Cdx-1 Cdx-2 are aberrantly expressed. The involvement in development their role several genes support hypothesis that and/or play important roles aberrant differentiation program carcinoma. To clarify mechanisms transcriptional regulation gene cells, pGL3 deletion constructs covering 2.6 kb human promoter were used transient transfection assays, enabling us to identify a relevant...
Changes in mucin protein expression and glycosylation are common features pre-neoplastic lesions cancer therefore used as cancer-associated markers. De novo of intestinal MUC2 sialyl-Tn antigen frequently observed metaplasia (IM) gastric cancer. However, despite that these antigens often co-localize, has not been demonstrated to be a carrier sialyl-Tn. By using the situ proximity ligation assay (in PLA), we herein could show is major all IM cases most carcinoma cases. The requirement by PLA...
The homeobox transcription factor CDX2 plays a crucial role in intestinal cell fate specification, both during normal development and tumorigenic processes involving reprogramming. regulatory network is intricate, but it has not yet been fully uncovered. Through genome-wide screening of 3D culture system, the RNA-binding protein MEX3A was identified as putatively involved regulation; therefore, its biological relevance addressed by setting up cell-based assays together with expression...
// Ming-Han Tsai 1, 2 , Xiaochen Lin 2, * Anatoliy Shumilov Katharina Bernhardt Regina Feederle 3 Remy Poirey Annette Kopp-Schneider 4 Bruno Pereira 5 Raquel Almeida Henri-Jacques Delecluse 1 German Cancer Research Centre (DKFZ), Unit F100, 69120 Heidelberg, Germany Inserm unit U1074, DKFZ, Institute for Diabetes and Obesitas, Monoclonal Antibody Core Facility, Center Environmental Health, Helmholtz Zentrum München, 81377 Munich, C060, Differentiation Group, IPATIMUP, Rua Dr Roberto...
Mucin glycoproteins are major secreted or membrane-bound molecules that, in cancer, show modifications both the mucin proteins expression and O-glycosylation profile, generating some of most relevant tumour markers clinical use for decades. Thus far, identification these biomarkers has been based on detection either protein O-glycan modifications. We therefore aimed to identify combined features, that is, specific glycoforms, an attempt increase specificity cancer biomarkers. Using situ...
Helicobacter pylori infection is the main risk factor for intestinal metaplasia (IM) and gastric cancer development. IM a pre-neoplastic lesion, induced by transcription CDX2, where mucosa converted to an phenotype. We previously demonstrated that key elements of bone morphogenetic protein (BMP) pathway co-localize with CDX2 in upregulate expression cell lines. These observations, together hypothesis could be repressed SOX2, led us test whether H. , through BMPs, SOX2 participate molecular...
We aimed to refine the value of CDX 2 as an independent prognostic and predictive biomarker in colorectal cancer ( CRC ) according disease stage chemotherapy sensitivity preclinical models. expression was evaluated 1045 I– IV primary s by gene n = 403) or immunohistochemistry 642) relation 5‐year relapse‐free survival RFS ), overall OS chemotherapy. Pharmacogenomic associations between 69 chemotherapeutics were assessed drug screening 35 cell lines. lost 11.6% cases showed poor multivariable...
Gastric cancer remains a serious health burden with few therapeutic options. Therefore, the recognition of stem cells (CSCs) as seeds tumorigenic process makes them prime target. Knowing that transcription factors SOX2 and OCT4 promote stemness, our approach was to isolate stem-like in human gastric cell lines using traceable reporter system based on SOX2/OCT4 activity (SORE6-GFP). Cells transduced SORE6-GFP were sorted into SORE6+ SORE6- populations, their biological behavior characterized....
Abstract Peritoneal dissemination is a particular form of metastasis typically observed in ovarian cancer and the major cause for poor patient’s outcome. Identification molecular players involved can offer an approach to develop treatment strategies improve clinical prognosis. Here, we identified mesothelin (MSLN) as crucial protein multistep process peritoneal cancer. We demonstrated that MSLN overexpressed primary matched high-grade serous carcinomas (HGSC). Using several genetically...
A novel putative member of the human UDP-galactose:β-<i>N</i>-acetylglucosamine β1,4-galactosyltransferase family, designated β4Gal-T4, was identified by BLAST analysis expressed sequence tags. The β4Gal-T4 encoded a type II membrane protein with significant similarity to other β1,4-galactosyltransferases. Expression full coding and secreted form in insect cells showed that gene product had activity. Analysis substrate specificity revealed enzyme catalyzed glycosylation glycolipids terminal...
Poly-N-acetyllactosamine is a unique carbohydrate composed of N-acetyllactosamine repeats and provides the backbone structure for additional modifications such as sialyl Lex. Poly-N-acetyllactosamines in mucin-type O-glycans can be formed core 2 branched oligosaccharides, which are synthesized by β-1,6-N-acetylglucosaminyltransferase.Using β-1,4-galactosyltransferase (β4Gal-TI) present milk recently cloned β-1,3-N-acetylglucosaminyltransferase, formation poly-N-acetyllactosamine was found to...
Helicobacter pylori infection induces intestinal metaplasia of the stomach, a preneoplastic lesion associated with an increased risk for gastric cancer development. Intestinal is induced by intestine-specific transcription factor CDX2 but mechanisms responsible this ectopic expression have never been described. We hypothesized that BMP/SMAD pathway has role in regulation, context, following reasons: (1) BMP crucial normal differentiation and (2) there influx BMP2 BMP4-producing cells to...
Aberrant mucin O-glycosylation is often observed in cancer and characterized by the expression of immature simple mucin-type carbohydrate antigens. UDP- N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-6 (ppGalNAc-T6) one enzymes responsible for initial step O-glycosylation. This study evaluated ppGalNAc-T6 human gastric mucosa, intestinal metaplasia, carcinomas. Our results showed that expressed normal mucosa metaplasia. A heterogeneous staining pattern this enzyme was...
<h3>Background and aims</h3> Intestinal metaplasia (IM) is a gastric preneoplastic lesion that appears following <i>Helicobacter pylori</i> infection confers an increased risk for development of cancer. It induced by expression the intestine-specific transcription factor CDX2. The regulatory mechanisms involved in triggering maintaining CDX2 have not been fully elucidated. Cdx2<sup>+/−</sup> mouse develops intestinal polyps with differentiation total loss Cdx2 absence structural second...