A5034014583- Nanoparticles: synthesis and applications
- 3D Printing in Biomedical Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Cellular Mechanics and Interactions
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Polymer Surface Interaction Studies
- Graphene and Nanomaterials Applications
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- Pickering emulsions and particle stabilization
- Nanocluster Synthesis and Applications
- Air Quality and Health Impacts
- Cancer Cells and Metastasis
- Phagocytosis and Immune Regulation
- Nanoplatforms for cancer theranostics
- Abdominal Trauma and Injuries
- Cell Adhesion Molecules Research
- bioluminescence and chemiluminescence research
- Additive Manufacturing and 3D Printing Technologies
- Advanced Fluorescence Microscopy Techniques
- Omental and Epiploic Conditions
- Photoreceptor and optogenetics research
- Lanthanide and Transition Metal Complexes
Adolphe Merkle Institute
2017-2023
University of Fribourg
2017-2023
Fribourg Development Agency
2020-2021
Institut de Science et d'Ingénierie Supramoléculaires
2014-2020
Université de Strasbourg
2009-2020
Centre National de la Recherche Scientifique
2014-2017
University of Cambridge
2014
Abstract The direct delivery of specific proteins to live cells promises a tremendous impact for biological and medical applications, from therapeutics genetic engineering. However, the process mostly involves tedious techniques often requires extensive alteration protein itself. Herein we report straightforward approach encapsulate native by using breakable organosilica matrices that disintegrate upon exposure chemical stimulus. biomolecule‐containing capsules were tested intracellular...
Mesoporous silica nanoparticles (MSNPs), 100 nm in size, incorporating a Cy5 fluorophore within the framework, are synthesized and loaded with anti-cancer drug temozolomide (TMZ), used treatment of gliomas. The surface particles is then decorated, using electrostatic interactions, polyarginine-peptide nucleic acid (R8-PNA) conjugate targeting miR221 microRNA. multi-functional nanosystem thus obtained rapidly internalized into glioma C6 or T98G cells. anti-miR activity PNA retained, as...
Expansion in production and commercial use of nanomaterials increases the potential human exposure during lifecycle these materials (production, use, disposal). Inhalation is a primary route to nanomaterials; therefore it critical assess their respiratory hazard. Herein, we developed three-dimensional alveolar model (EpiAlveolar) consisting epithelial cells, fibroblasts, endothelial with or without macrophages for predicting long-term responses aerosols. Following thorough characterization...
The extracellular matrix (ECM) plays critical roles in tumor progression and metastasis. However, the contribution of ECM proteins to early metastatic onset peritoneal cavity remains unexplored. Here, we suggest a new route metastasis through interaction integrin alpha 2 (ITGA2) with collagens enriched coinciding poor outcome patients ovarian cancer. Using multiple gene-edited cell lines patient-derived samples, demonstrate that ITGA2 triggers cancer adhesion collagen, promotes migration,...
Self-assembled platinum compounds resulting in stable, highly emissive and long-lived species are reported for cell imaging.
An emerging field regarding N^C^N terdentate Pt(II) complexes is their application as luminescent labels for bio-imaging. In fact, phosphorescent Pt possess many advantages such a wide emission color tunability, better stability towards photo- and chemical degradation, very large Stokes shift, long-lived excited states with lifetimes typically two to three orders of magnitude longer than those classic organic fluorophores. Here, we describe the synthesis photophysical characterization new...
The long-term fate of biomedically relevant nanoparticles (NPs) at the single cell level after uptake is not fully understood yet. We report that lysosomal exocytosis NPs a mechanism to reduce particle load. Biopersistent such as nonporous silica and gold remain in cells for prolonged time. only reduction intracellular NP number observed via division, e.g., mitosis. Additionally, distribution division be asymmetrical, likely due inhomogeneous location NP-loaded vesicles mother cells. These...
In vitro three-dimensional (3D) lung cell models have been thoroughly investigated in recent years and provide a reliable tool to assess the hazard associated with nanomaterials (NMs) released into air. this study, 3D co-culture model was optimized potential of multiwalled carbon nanotubes (MWCNTs), which is known provoke inflammation fibrosis, critical adverse outcomes linked acute prolonged NM exposure. The co-cultures were exposed MWCNTs at air-liquid interface (ALI) using VITROCELL®...
The design and synthesis of smart nanomaterials can provide interesting potential applications for biomedical purposes from bioimaging to drug delivery. Manufacturing multifunctional systems in a way carry bioactive molecules, like peptide nucleic acids able recognize specific targets living cells, represents an achievement towards the development highly selective tools both diagnosis therapeutics. This work describes very first example use zeolite nanocrystals as nanocarriers deliver...
The lung represents the primary entry route for airborne particles into human body. Most studies addressed possible adverse effects using single (nano)particles, but aerosolic nanoparticles (NPs) tend to aggregate and form structures of several hundreds nm in diameter, changing physico-chemical properties interaction with cells. Our aim was investigate how aggregation might affect biodistribution; cellular uptake translocation over time aerosolized NPs at air-blood barrier interface a...
Abstract The design and synthesis of multifunctional nanomaterials could lead to applications relevant for biomedicine. Manufacturing porous particles make them able carry bioactive molecules into living cells represents a substantial goal towards the development powerful tools nanomedicine. This work describes first example using zeolite‐L crystals as nanocontainers simultaneously deliver DNA oligonucleotides organic cells. Multifunctional was prepared by filling pore system with guest...
New luminescent glycosilated rhenium complexes were synthesized and evaluated as dyes for optical imaging.
This work aims to establish a production and characterization protocol for fluorescent plastic nanoparticles of poly(ethylene terephthalate) (PET), polypropylene (PP), polystyrene (PS) that can be tracked in biological environments.
Cells continuously exert forces on their environment and respond to changes in mechanical by altering behaviour.
Polydopamine can form biocompatible particles that convert light into heat. Recently, a protocol has been optimized to synthesize polydopamine/protein hybrid nanoparticles retain the biological function of proteins, and combine it with stimuli-induced heat generation polydopamine. We have utilized this novel system polydopamine particles, containing transferrin (PDA/Tf). Mouse melanoma cells, which strongly express receptor, were exposed PDA/Tf (NPs) and, subsequently, irradiated UV laser....
Introduction: The expanding use of multiwalled carbon nanotubes (MWCNTs) in various consumer and industrial products has raised concerns regarding the potential health risks following inhalation this nanomaterial. Recent studies indicate that MWCNT exposure can cause deleterious lung effects. Pulmonary fibrosis is one diseases associated with classical fiber-shaped materials (e.g., asbestos), recently MWCNTs. Materials Methods: Herein, presented work investigated early signs key human cell...
Abstract Lasing from cells has recently been subject of thorough investigation because the potential for sensitive and fast biosensing. Yet, lasing individual studied in high‐quality resonators, resulting limited dependence properties on cellular microenvironment. Here, is triggered by floating a low quality factor resonator composed disposable poly(methyl methacrylate) (PMMA) cell counting‐slide, hence absence conventional high‐reflectivity optical cavities. The exceptional spectral...
Reliable and predictive experimental models are urgently needed to study metastatic mechanisms of ovarian cancer cells in the omentum. Although for cell adhesion invasion were previously investigated, lack certain omental types, which influence behavior cells, limits application these tissue models. Here, we describe a 3D multi-cellular human omentum model, considers spatial arrangement five types. Reproducible fabricated combining permeable culture inserts bioprinting technology mimic...
Abstract The direct delivery of specific proteins to live cells promises a tremendous impact for biological and medical applications, from therapeutics genetic engineering. However, the process mostly involves tedious techniques often requires extensive alteration protein itself. Herein we report straightforward approach encapsulate native by using breakable organosilica matrices that disintegrate upon exposure chemical stimulus. biomolecule‐containing capsules were tested intracellular...