A5021313313- Microtubule and mitosis dynamics
- Bone Tissue Engineering Materials
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Plant Molecular Biology Research
- Biosensors and Analytical Detection
- Polysaccharides and Plant Cell Walls
- Graphene and Nanomaterials Applications
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Cellular Mechanics and Interactions
- Advanced Biosensing Techniques and Applications
- Cellular transport and secretion
- Protist diversity and phylogeny
- Genetics, Bioinformatics, and Biomedical Research
- Near-Field Optical Microscopy
- Advanced Drug Delivery Systems
- Analytical chemistry methods development
- Electron and X-Ray Spectroscopy Techniques
- Polymer Surface Interaction Studies
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- Proteins in Food Systems
- Genomics and Chromatin Dynamics
- Polysaccharides Composition and Applications
Universidade do Porto
2009-2025
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2025
Instituto de Biologia Molecular e Celular
2005-2024
Peking University
2022
University of Limerick
2022
Italian Institute of Technology
2022
University of Pisa
2022
Universidad Complutense de Madrid
2022
University of Wisconsin–Madison
2022
University of California, Irvine
2022
The actin-binding protein adducin was recently identified as a component of the neuronal subcortical cytoskeleton. Here, we analyzed mice lacking to uncover function this in actin rings. α-adducin knockout presented progressive axon enlargement spinal cord and optic sciatic nerves, followed by degeneration loss. Using stimulated emission depletion super-resolution microscopy, show that periodic cytoskeleton is assembled every neuron type inspected including retinal ganglion cells dorsal root...
Neurons have a membrane periodic skeleton (MPS) composed of actin rings interconnected by spectrin. Here, combining chemical and genetic gain- loss-of-function assays, we show that in rat hippocampal neurons the MPS is an actomyosin network controls axonal expansion contraction. Using super-resolution microscopy, analyzed localization non-muscle myosin II (NMII). We active NMII light chains are colocalized with organized circular manner throughout axon shaft. In contrast, heavy mostly...
Helicobacter pylori induces an invasive phenotype in gastric epithelial cells through a mechanism that requires the type IV secretion system and phosphorylation of c-Met. The E-cadherin-catenin complex is major component adherens junctions functions as invasion suppressor. We investigated whether E-cadherin has role H. pylori-induced, c-Met phosphorylation-dependent cell-invasive phenotype.AGS lack are to stimulation were transduced with infected pylori. NCI-N87 cells, which endogenously...
Multiple asters (MAST)/Orbit is a member of new family nonmotor microtubule-associated proteins that has been previously shown to be required for the organization mitotic spindle. Here we provide evidence MAST/Orbit functional kinetochore attachment, chromosome congression, and maintenance spindle bipolarity. In vivo analysis Drosophila mast mutant embryos undergoing early divisions revealed chromosomes are unable reach stable metaphase alignment bipolar spindles collapse as centrosomes move...
Abstract This study aims at assessing the influence of competitive preadsorption human serum albumin (HSA) and plasma fibronectin (FN) from binary solutions 10% on MC3T3‐E1 osteoblast adhesion morphology two types TiO 2 substrates. One was commercially pure titanium with a oxide layer formed in an H O solution other sputtered Si (Sousa et al., Langmuir 2004; 20:9745–9754.). The strategy applied present investigation to compare surfaces preadsorbed HSA, FN, HSA/FN = 1, 200, plasma. adsorption...
Chitosan (Ch) is being actively investigated as a non-protein template for the growth of an increasing number anchorage-dependent cells, including chondrocytes and bone cells. In present work, Ch films with degrees N-acetylation (DAs) in range 4 to 49% were evaluated respect attachment, spreading short-term proliferation osteoblasts, using human osteoblastic MG-63 The characterized terms surface morphology charge by atomic force microscopy streaming potential measurements, respectively. Cell...
The aim of this work was to develop a bioactive bone substitute with an effective antibacterial ability based on cerium (Ce) doped glass-reinforced hydroxyapatite (GR-HA) composite. Developed composites were physicochemically characterized, using x-ray diffraction (XRD) analysis, SEM, energy dispersive spectroscopy (EDS), and flexural bending strength (FBS) tests. X-ray photoelectron (XPS) analysis performed analyze the oxidation state Ce in prepared glass. antimicrobial activity evaluated...
Epilepsy is a highly prevalent neurological disease and valproic acid (VPA) used as first-line chronic treatment. However, this drug has poor oral bioavailability, which requires the administration of high doses, resulting in adverse effects. Alternative routes VPA have therefore been investigated, such nose-to-brain route, allows to be transported directly from nasal cavity brain. Here, use nanostructured lipid carriers (NLC) encapsulate drugs administered proved advantageous. The aim work...
Abstract In this investigation, the effect of degree acetylation (DA) chitosan on behavior human osteoblastic MG‐63 cells cultured in three‐dimensional matrices was assessed. Chitosan sponges with DAs range 4 to 49% were prepared and characterized terms microstructure, porosity, pore size. Collagen used as 3D control. Cell proliferation determined using MTT assay while retention phenotype monitored by assaying alkaline phosphatase activity. morphology, cytoskeletal organization, viability...
Abstract Cell adhesion, migration, and proliferation of a few anchorage‐dependent cells cultured on chitosan (Ch) matrices are influenced by the degree N ‐acetylation (DA) Ch. In present work, we examined influence DA attachment, spreading, proliferation, osteogenic differentiation rat bone marrow stromal (rBMSCs). Ch membranes were characterized in terms surface morphology, roughness, wettability, adsorption an adhesive protein, fibronectin (Fn). Chs with DAs range 4 to 49% used. Among...
γ-Tubulin is critical for the initiation and regulation of microtubule (MT) assembly. In Drosophila melanogaster, it acts within two main complexes: γ-tubulin small complex (γ-TuSC) ring (γ-TuRC). Proteins specific γ-TuRC, although nonessential viability, are required efficient mitotic progression. Until now, their role during interphase remained poorly understood. Using RNA interference in S2 cells, we show that γ-TuRC not overall MT organization. However, depletion any component this...
Stimulated emission depletion (STED) fluorescence microscopy squeezes an excited spot well below the wavelength scale using a doughnut-shaped beam. To generate doughnut, scale-free vortex phase modulation (2D-STED) is often used because it provides maximal transverse confinement and radial-aberration immunity (RAI) to central dip. However, RAI also means blindness defocus term, making axial origin of photons uncertain within provided by confocal detection pinhole. Here, reduce uncertainty,...
The gene polo encodes a highly conserved serine/threonine protein kinase that has been implicated in several functions during cell division. Polo-like kinases are important positive regulators of cycle progression and have also the exit from mitosis through activation anaphase-promoting complex. Several data indicate Plks required for centrosome function, bipolar spindle organisation cytokinesis. intracellular localisation reflects their multiple roles division, however, vivo studies...
Abstract An important group of microtubule associated proteins are the plus‐end tracking which includes Mast/Orbit/CLASPs family amongst others. Several these have functions during interphase and mitosis in modulation dynamic properties microtubules, however, precise mechanism remains to be elucidated. To investigate role Mast regulation behaviour interphase, we used RNAi Drosophila S2 culture cells stably expressing GFP‐α‐tubulin followed microtubules vivo. depleted show a significant...
In this work, the conjugation of molecularly imprinted polymers (MIPs) to quantum dots (QDs) was successfully applied in assembly an cellulose membrane [hydroxy ethyl (HEC)/MIP@QDs] for specific recognition cardiac biomarker myoglobin (Myo) as a sensitive, user-friendly, and portable system with potential point-of-care (POC) applications. The concept is use MIPs biorecognition elements, previously prepared on surface semiconductor cadmium telluride QDs detection particles. fluorescent...
Nanocarriers (NCs) have emerged as a revolutionary approach in targeted drug delivery, promising to enhance efficacy and reduce toxicity through precise targeting controlled release mechanisms. Despite their potential, the clinical adoption of NCs is hindered by challenges physicochemical characterization, essential for ensuring safety, efficacy, quality control. Traditional characterization methods, such dynamic light scattering nanoparticle tracking analysis, offer limited insights,...