A5090022438- Insect Resistance and Genetics
- Insect and Pesticide Research
- Bacillus and Francisella bacterial research
- Invertebrate Immune Response Mechanisms
- Helicobacter pylori-related gastroenterology studies
- Escherichia coli research studies
- Glycosylation and Glycoproteins Research
- Insect symbiosis and bacterial influences
- Peptidase Inhibition and Analysis
- Bacterial Genetics and Biotechnology
- Galectins and Cancer Biology
- Salmonella and Campylobacter epidemiology
- Probiotics and Fermented Foods
- Identification and Quantification in Food
- Gastroesophageal reflux and treatments
- Enzyme Structure and Function
- Protein Structure and Dynamics
- RNA Interference and Gene Delivery
- Carbohydrate Chemistry and Synthesis
- Microplastics and Plastic Pollution
- Antibiotic Resistance in Bacteria
- Insect Pest Control Strategies
- Insect Utilization and Effects
- Meat and Animal Product Quality
- Genomics and Phylogenetic Studies
Agence Nationale des Fréquences
2021-2024
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2022-2024
Paris-Est Sup
2024
Institut Pasteur
2010-2014
Fondation de l'Avenir
2010-2014
Inserm
2010-2014
The molecular basis of the regulation specific shapes and their role for bacterial fitness remain largely unknown. We focused in this study on Gram-negative spiral-shaped Helicobacter pylori. To colonize its unique niche, H. pylori needs to reach quickly human gastric mucosa, by swimming through mucus layer. For that reason, shape is predicted be necessary optimal motility vivo, consequently colonization ability. Here, we describe involvement a PG-modifying enzyme, HdpA (HP0506), mouse...
The flagellar machinery is a highly complex organelle composed of free rotating flagellum and fixed stator that converts energy into movement. assembly the flagella requires interactions with peptidoglycan layer through which has to pass for externalization. Lytic transglycosylases are degrading enzymes cleave sugar backbone layer. We show an endogenous lytic transglycosylase required full motility Helicobacter pylori colonization gastric mucosa. Deficiency resulted from paralysed phenotype...
Bacillus thuringiensis (Bt) belongs to the cereus (Bc) group, well known as an etiological agent of foodborne outbreaks (FBOs). Bt distinguishes itself from other Bc by its ability synthesize insecticidal crystals. However, search for these crystals is not routinely performed in food safety or clinical investigation, and actual involvement occurrence FBOs known. In present study, we reveal that was detected context 49 declared France between 2007 2017. 19 FBOs, only microorganism detected,...
Bacillus cereus (Bc) is a wide group of Gram-positive and spore-forming bacteria, known to be the etiological agents various human infections, primarily food poisoning. The Bc includes enteropathogenic strains able germinate in digestive tract produce enterotoxins such as Nhe, Hbl, CytK. One species group, thuringiensis (Bt), has unique feature producing insecticidal crystals during sporulation, making it an important alternative chemical pesticides protect crops from insect pest larvae....
Bacillus cereus sensu lato (Bcsl) is a group of closely related bacterial species known for their resistant spores, enabling them to persist in dormant state and thereby colonize adapt across diverse environments. Bcsl its harmful impact on human health, producing toxins that cause emetic diarrheal syndromes or provoking opportunistic infections hospitals. Importantly, the most frequent confirmed presumptive causative agent associated with foodborne outbreaks (FBOs) France. In our study, we...
H. pylori colonizes half of the world's population leading to gastritis, ulcers and gastric cancer. strains resistant antibiotics are increasing which raises need for alternative therapeutic approaches. Docosahexaenoic acid (DHA) has been shown decrease growth its associated-inflammation through mechanisms poorly characterized. We aimed explore DHA action on pylori-mediated inflammation adhesion epithelial cells (AGS) also identify bacterial structures affected by DHA. metabolism was...
Helicobacter pylori, a human-specific bacterial pathogen responsible for severe gastric diseases, constitutes major public health issue. In the last decade, rates of H. pylori resistance to antibiotics were increasing drastically, requiring alternative therapeutic strategies deal with eradication failures. Therefore, we evaluated potential bulgecin A, glycosidic inhibitor lytic transglycosylase (LTG) Slt70 Escherichia coli, as new approach against infection. this study, show that A is able...
Bacillus thuringiensis subsp. kurstaki ( Btk ) is a strong pathogen toward lepidopteran larvae thanks to specific Cry toxins causing leaky gut phenotypes. Hence, and its are used worldwide as microbial insecticide in genetically modified crops, respectively, fight crop pests. However, belongs the B. cereus group, some strains of which well known human opportunistic pathogens. Therefore, ingestion along with food may threaten organisms not susceptible infection. Here we show that Cry1A induce...
Peptidoglycan O-acetylesterase (Ape1), which is required for host survival in Neisseria sp., belongs to the diverse SGNH hydrolase superfamily, includes important viral and bacterial virulence factors. Here, multi-domain crystal structures of Ape1 with an catalytic domain a newly identified putative peptidoglycan-detection module are reported. Enzyme catalysis was performed crystals key intermediates along esterase hydrolysis reaction pathway were visualized, revealing substrate-induced...
In the context of pathogen surveillance, it is crucial to ensure interoperability and harmonized data. Several surveillance systems are designed compare bacteria identify outbreak clusters based on core genome MultiLocus Sequence Typing (cgMLST). Among different approaches available generate bacterial cgMLST, our research used an assembly-based approach (chewBBACA tool).
Bacillus thuringiensis (Bt), belonging to the cereus (Bc) group, is commonly used as a biopesticide worldwide due its ability produce insecticidal crystals during sporulation. The use of Bt, especially subspecies aizawai and kurstaki, control pests such Lepidoptera, generally involves spraying mixtures containing spores on crops intended for human consumption. Recent studies have suggested that consumption commercial Bt strains may be responsible foodborne outbreaks (FBOs). However, genetic...
SUMMARY Spores are considered as dormant entities highly resistant to extreme conditions. Among them, Bacillus cereus spores commonly associated with foodborne outbreaks. Nevertheless, the pathological processes spore ingestion and germination remain poorly understood. Here, we show that while of vegetative bacteria leads their elimination from midgut small intestines Drosophila mice, respectively, a single persistence for at least 10 days. Using genetics, demonstrate escape innate immune...
Soumission à Epi-revel D'après les travaux issus du projet BtSI2S soutenu par l'Académie 3, Université Côte d'Azur et publiés dans: Salma Hachfi, Alexandra Brun-Barale, Arnaud Fichant, Patrick Munro, Marie-Paule Nawrot-Esposito, Gregory Michel, Raymond Ruimy, Raphaël Rousset; Mathilde Bonis, Laurent Boyer and Armel Gallet. "Ingestion of Bacillus cereus spores dampens the immune response to favor bacterial persistence" (2023). bioRxiv (Nature Comm)...
<title>Abstract</title> Background In the context of pathogen surveillance, it is crucial to ensure interoperability and harmonized data. Several surveillance systems are designed compare bacteria identify outbreak clusters based on core genome MultiLocus Sequence Typing (cgMLST). Among different approaches available generate bacterial cgMLST, our research used an assembly-based approach (chewBBACA tool). Methods Simulations short-read sequencing were conducted for 5 genomes 27 pathogens...
In Figure 4 and S9, the panels were initially built as a single field with pasted representative images of each strain. However, use such composite is against journal policy, we apologize for this mistake. The two figures remade according to journal's policy panel now composed same presented individually. These corrections do not affect our conclusions on differential localisation GFP-hpMotB between wild type E. coli their corresponding lytic transglycosylase mutants, these individual part...
Abstract Bacillus thuringiensis subsp. kurstaki ( Btk ) is a strong pathogen toward lepidopteran larvae thanks to specific Cry toxins causing leaky gut phenotypes. Hence, and its are used worldwide as microbial insecticide in genetically modified crops, respectively, fight crop pests. However, belongs the B. cereus group, some strains of which well known human opportunistic pathogens. Therefore, ingestion along with food may threaten organisms not susceptible infection. Here we show that...
Abstract Bacillus thuringiensis (Bt), belonging to the cereus (Bc) group, is commonly used as a biopesticide worldwide, due its ability produce insecticidal protein crystals during sporulation. The use of Bt, especially subspecies aizawai and kurstaki , control pests such Lepidoptera generally involves spraying mixtures containing spores on crops intended for human consumption. Recent studies have suggested that consumption commercial Bt strains may be responsible foodborne outbreaks (FBOs)....