A5078798672- Genetics, Aging, and Longevity in Model Organisms
- Animal Virus Infections Studies
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- Tryptophan and brain disorders
- Microbial Metabolic Engineering and Bioproduction
- Viral Infections and Immunology Research
- Respiratory viral infections research
- interferon and immune responses
- Circadian rhythm and melatonin
- RNA modifications and cancer
- Bioinformatics and Genomic Networks
- Genomics, phytochemicals, and oxidative stress
- Influenza Virus Research Studies
- Cardiac Arrhythmias and Treatments
- Animal Genetics and Reproduction
- Cardiovascular Syncope and Autonomic Disorders
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Metabolomics and Mass Spectrometry Studies
- Pharmacogenetics and Drug Metabolism
- Mitochondrial Function and Pathology
- Viral Infections and Outbreaks Research
Université de Versailles Saint-Quentin-en-Yvelines
2022-2023
Inserm
2022-2023
Université Paris-Saclay
2022-2023
Institut Pasteur
2013-2023
Université Paris Cité
2013-2023
Infection et inflammation
2023
University of Massachusetts Chan Medical School
2018-2022
Institut thématique Immunologie, inflammation, infectiologie et microbiologie
2022
Université de Montréal
2017
Montreal Clinical Research Institute
2017
Cells are highly asymmetrical, a feature that relies on the sorting of molecular constituents, including proteins, lipids, and nucleic acids, to distinct subcellular locales. The localization RNA molecules is an important layer gene regulation required modulate localized cellular activities, although its global prevalence remains unclear. We combine biochemical cell fractionation with RNA-sequencing (CeFra-seq) analysis assess conservation asymmetric distribution transcriptome-wide scale in...
A precise mapping of pathogen–host interactions is essential for comprehensive understanding the processes infection and pathogenesis. The most frequently used techniques interactomics are yeast two-hybrid binary methodologies, which do not recapitulate pathogen life cycle, tandem affinity purification mass spectrometry co-complex cannot distinguish direct from indirect interactions. New technologies thus needed to improve In current study, we detected between influenza virus polymerase host...
Abstract In our group, we aim to understand metabolism in the nematode Caenorhabditis elegans and its relationships with gene expression, physiology response therapeutic drugs. On March 15, 2020, a stay-at-home order was put into effect state of Massachusetts, USA, flatten curve spread novel SARS-CoV2 virus that causes COVID-19. For biomedical researchers state, this meant putting hold on experiments for nine weeks until May 18, 2020. To keep lab engaged productive, enhance communication...
Abstract Enhancing the knowledge of host factors that are required for efficient influenza A virus (IAV) replication is essential to address questions related pathogenicity and identify targets antiviral drug development. Here we focused on interplay between IAV DExD-box RNA helicases (DDX), which play a key role in cellular metabolism by remodeling RNA-RNA or RNA-protein complexes. We performed targeted RNAi screen 35 human DDX proteins those involved life cycle. DDX19 was major hit. In...
Abstract In our group, we aim to understand metabolism in the nematode Caenorhabditis elegans and its relationships with gene expression, physiology, response therapeutic drugs. Visualization of metabolic pathways that comprise network is extremely useful for interpreting a wide variety experiments. Detailed annotated pathway maps C. are mostly limited pan-organismal maps, many incomplete or inaccurate enzyme annotations. Here, present WormPaths, which composed two parts: (1) careful manual...
Tamoxifen is a selective estrogen receptor (ER) modulator that used to treat ER-positive breast cancer, but at high doses kills both and ER-negative cancer cells. We recapitulate this off-target effect in Caenorhabditis elegans, which does not have an ER ortholog. find different bacteria dramatically modulate tamoxifen toxicity C. with three-order of magnitude difference between animals fed Escherichia coli, Comamonas aquatica, Bacillus subtilis. Remarkably, host fatty acid (FA) biosynthesis...
Respiratory syncytial virus (RSV) RNA synthesis takes place in cytoplasmic viral factories also called inclusion bodies (IBs), which are membrane-less organelles concentrating the polymerase complex. The assembly of IBs is driven by liquid-liquid phase separation promoted interactions between nucleoprotein N and phosphoprotein P. We recently demonstrated that cyclopamine (CPM) inhibits RSV multiplication disorganizing hardening IBs. Although a single mutation transcription factor M2-1...
The maintenance of optimal membrane composition under basal and stress conditions is critical for the survival an organism. High-glucose has been shown to perturb properties by decreasing fluidity, sensor PAQR-2 required restore integrity. However, mechanisms respond elevated dietary glucose are not fully established. In this study, we used a 13C stable isotope-enriched diet mass spectrometry better understand impact on fatty acid dynamics in Caenorhabditis elegans. We found novel role...
Respiratory syncytial virus (RSV) RNA synthesis takes place in cytoplasmic viral factories also called inclusion bodies (IBs), which are membrane-less organelles concentrating the polymerase complex. IBs assembly is driven by liquid-liquid phase separation promoted interactions between nucleoprotein N and phosphoprotein P. We recently demonstrated that cyclopamine (CPM) inhibits RSV multiplication disorganizing hardening IBs. Although a single mutation transcription factor M2-1 induced...
Abstract Tamoxifen is a selective estrogen receptor (ER) modulator that used to treat ER-positive breast cancer, but at high doses kills both and ER-negative cancer cells. We recapitulate this off-target effect in Caenorhabditis elegans , which does not have an ER ortholog. find different bacteria dramatically modulate tamoxifen toxicity C. with three-order of magnitude difference between animals fed Escherichia coli Comamonas aquatica Bacillus subtilis . Remarkably, host fatty acid (FA)...