A5000860597- NF-κB Signaling Pathways
- Acute Myeloid Leukemia Research
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Cell death mechanisms and regulation
- Acute Lymphoblastic Leukemia research
- Cell Adhesion Molecules Research
- Protein Tyrosine Phosphatases
- Immunotherapy and Immune Responses
- Chronic Myeloid Leukemia Treatments
- RNA modifications and cancer
- Chronic Lymphocytic Leukemia Research
- Cancer Research and Treatments
- Probiotics and Fermented Foods
- T-cell and Retrovirus Studies
- Peptidase Inhibition and Analysis
- interferon and immune responses
- Microbial Metabolism and Applications
- Enzyme function and inhibition
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Natural product bioactivities and synthesis
- Cancer, Hypoxia, and Metabolism
- Virus-based gene therapy research
Inserm
2011-2024
Université Côte d'Azur
2011-2024
Centre Méditerranéen de Médecine Moléculaire
2010-2024
Hôpital Pasteur
1996
Melanoma cells can enter the process of senescence, but whether they express a secretory phenotype, as reported for other cells, is undetermined. This paramount importance, because this secretome alter tumor microenvironment and response to chemotherapeutic drugs. More generally, molecular events involved in formation senescent-associated have yet be determined. We reveal here that melanoma experiencing senescence diverse stimuli, including anti-melanoma drugs, produce an inflammatory...
Enterohemorrhagic Escherichia coli (EHEC) infections are associated with the modification of tight-junction permeability and synthesis proinflammatory cytokine interleukin-8 (IL-8). In a previous study, it was demonstrated that EHEC-induced IL-8 secretion is due to involvement mitogen-activated protein kinase (MAPK), AP-1, NF-kappaB pathways. this we investigated effect yeast Saccharomyces boulardii on EHEC infection in T84 cells. For purpose, cells were (i) incubated bacteria at same time...
Rapid tyrosine phosphorylation of key cellular proteins is a crucial event in the transduction activation signals to T-lymphocytes. The regulatory role protein phosphatases (PTPases) this process was explored by studying effects powerful PTPase inhibitor, vanadate peroxide (pervanadate), on cascade Jurkat human leukaemic T-cells. Pervanadate induced kinases lck and fyn (4- 3-fold respectively) dramatic increase proteins, notably phospholipase C gamma 1. After event, we observed rise...
Salmonella pathogenesis engages host cells in two-way biochemical interactions: phagocytosis of bacteria by recruitment cellular small GTP-binding proteins induced the bacteria, and triggering a pro-inflammatory response through activation MAPKs nuclear translocation NF-kappaB. Worldwide interest use functional foods containing probiotic for health promotion disease prevention has increased significantly. Saccharomyces boulardii is non-pathogenic yeast used as infectious diarrhea.In this...
Many antibiotics in clinical use target the bacterial ribosome by interfering with protein synthesis machinery. However, targeting human case of deregulations such as highly proliferating cancer cells has not been investigated at molecular level up to now. Here we report structure 80S a eukaryote-specific antibiotic and show its anti-proliferative effect on several cell lines. The provides insights into detailed interactions ligand-binding pocket that are required for structure-assisted drug...
The mechanisms that regulate keratinocyte migration and proliferation in wound healing remain largely unraveled, notably regarding possible involvements of microRNAs (miRNAs). Here we disclose up-regulation miR-483-3p 2 distinct models healing: scratch-injured cultures human keratinocytes wounded skin mice. accumulation peaks at the final stage closure process, consistent with a role arrest "healing" progression. Using an vitro wound-healing model, videomicroscopy, 5-bromo-2'-uridine...
Phosphorylation of the N‐terminal domain IκB inhibitory subunits induces activation transcription factor NF‐κB. Although serine phosphorylation has been shown to induce ubiquitination and subsequent proteasome‐mediated degradation IκB‐α, little is known about mechanisms that lead release active NF‐κB in T cells as a consequence tyrosine IκB‐α[Imbert, V., Rupec, R.A., Livolsi, A., Pahl, H.L., Traenckner, B.M., Mueller‐Dieckmann, C., Farahifar, D., Rossi, B., Auberger, P., Baeuerle, P. &...
Hepatitis C virus nonstructural protein 5A (NS5A) has been implicated in the HCV antiviral resistance, replication, and transactivation of cellular gene expression. We have recently shown that NS5A activates NF-κB via oxidative stress (22Gong G. Waris Tanveer R. Siddiqui A. Proc. Natl. Acad. Sci. U. S. 2001; 98: 9599-9604Crossref PubMed Scopus (544) Google Scholar). In this study, we investigate molecular mechanism(s) activation response to induced by protein. contrast classic Ser32,36...
ABSTRACT Enterohemorrhagic Escherichia coli (EHEC) infections are associated with hemorrhagic colitis and the hemolytic-uremic syndrome (HUS). In vivo, elevated plasma levels of proinflammatory cytokine interleukin-8 (IL-8) in EHEC-infected children correlated a high risk developing HUS. As IL-8 gene transcription is regulated by factors NF-κB AP-1, we analyzed role these regulation production after infection epithelial intestinal T84 cell line EHEC. By 6 h infection, EHEC had induced...
Specific BRAFV600E inhibitors (BRAFi) are highly effective in the treatment of melanoma. However, acquired drug resistances invariably develop after initial response. Therefore, identification new mechanisms resistance gives important clues towards development therapies that could elicit long lasting responses. Here we report CD271 confers to BRAFi melanoma cells. The expression is increased by through a stimulation tumor necrosis factor-alpha (TNFα) secretion leads NF-κB signaling pathway...
Thrombin stimulation of the T leukemic cell line Jurkat induced a transient increase in [Ca2+]i. Proteolytic activity enzyme was required for this effect since diisopropyl fluorophosphate-thrombin failed to Furthermore, hirudin and anti-thrombin III inhibited thrombin-induced [Ca2+]i rise cells. A synthetic thrombin receptor agonist peptide (TRP) 7 residues (SFLLRNP) found be as effective mobilization, both agonists Ca2+ release exclusively from internal stores. stimulated tyrosine...