A5002265683- Virus-based gene therapy research
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- T-cell and B-cell Immunology
- Lymphoma Diagnosis and Treatment
- T-cell and Retrovirus Studies
- Virology and Viral Diseases
- Chronic Myeloid Leukemia Treatments
- Acute Lymphoblastic Leukemia research
- Cytomegalovirus and herpesvirus research
- Chronic Lymphocytic Leukemia Research
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Ubiquitin and proteasome pathways
- Immunodeficiency and Autoimmune Disorders
- Acute Myeloid Leukemia Research
- RNA modifications and cancer
- Parvovirus B19 Infection Studies
- Single-cell and spatial transcriptomics
- Biomedical Ethics and Regulation
Centre International de Recherche en Infectiologie
2016-2025
Université Claude Bernard Lyon 1
2016-2025
Inserm
2016-2025
École Normale Supérieure de Lyon
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Centre Méditerranéen de Médecine Moléculaire
2016-2025
Université Côte d'Azur
2017-2025
La Ligue Contre le Cancer
2020-2025
Observatoire de la Côte d’Azur
2025
Université de Lyon
2021-2024
Programmable nucleases have enabled rapid and accessible genome engineering in eukaryotic cells living organisms. However, their delivery into target can be technically challenging when working with primary or vivo. Here, we use engineered murine leukemia virus-like particles loaded Cas9-sgRNA ribonucleoproteins (Nanoblades) to induce efficient genome-editing cell lines including human induced pluripotent stem cells, hematopoietic mouse bone-marrow cells. Transgene-free Nanoblades are also...
Mitophagy is an evolutionarily conserved process that selectively targets impaired mitochondria for degradation. Defects in mitophagy are often associated with diverse pathologies, including cancer. Because the main known regulators of frequently inactivated cancer cells, mechanisms regulate cells not fully understood. Here, we identified E3 ubiquitin ligase (ARIH1/HHARI) triggers a PINK1-dependent manner. We found ARIH1/HHARI polyubiquitinates damaged mitochondria, leading to their removal...
Abstract CSF-1 and IL-34 share the receptor no differences have been reported in signaling pathways triggered by both ligands human monocytes. promotes differentiation survival of monocytes, macrophages osteoclasts, as does. However, binds other receptors, suggesting that exist effect cytokines. In present study, we compared polarization abilities primary monocytes response to or IL-34. CSF-1R engagement one leads AKT caspase activation autophagy induction through expression AMPK ULK1. As...
Cell cycle entry is commonly considered to positively regulate HIV-1 infection of CD4 T cells, raising the question as how quiescent lymphocytes, representing a large portion viral reservoir, are infected in vivo. Factors such homeostatic cytokine IL-7 have been shown render cells permissive infection, presumably by transiently stimulating their into cell cycle. However, we show here that at physiological oxygen (O 2 ) levels (2–5% O tension lymphoid organs), stimulation generates an...
Chimeric antigen receptor (CAR) T cells brought substantial benefit to patients with B-cell malignancies. Notwithstanding, CAR T-cell manufacturing requires complex procedures impeding the broad supply chain. Here, we provide evidence that human CD19-CAR can be generated directly
NK-cell resistance to transduction is a major technical hurdle for developing immunotherapy. By using Baboon envelope pseudotyped lentiviral vectors (BaEV-LVs) encoding eGFP, we obtained rate of 23.0 ± 6.6% (mean SD) in freshly-isolated human NK-cells (FI-NK) and 83.4 10.1% from the Activation Expansion System (NKAES), with sustained transgene expression at least 21 days. BaEV-LVs outperformed Vesicular Stomatitis Virus type-G (VSV-G)-, RD114- Measles (MV)- LVs (p < 0.0001). mRNA both BaEV...
The success and limitations of current immunotherapies have pushed research toward the development alternative approaches possibility to manipulate other cytotoxic immune cells such as natural killer (NK) cells. Here, we targeted an intracellular inhibiting protein 'cytokine inducible SH2-containing protein' (CISH) in NK evaluate impact on their functions antitumor properties.To further understand CISH cells, developed a conditional Cish-deficient mouse model (Cishfl/flNcr1Ki/+ ). cytokine...
CAR NK cells as vehicles for engineered "off-the-shelf" cellular cancer immunotherapy have attracted significant interest. Nonetheless, a comprehensive comparative assessment of the anticancer activity T and carrying approved benchmark anti-CD19 constructs is missing. Here, we report direct head-to-head comparison CD19-directed human cells. We generated derived from healthy donor PBMC by retroviral transduction with same second-generation construct, FMC63.28z. investigated IFN-γ secretion...
Although the conserved AAA ATPase and bromodomain factor, ATAD2, has been described as a transcriptional co-activator upregulated in many cancers, its function remains poorly understood. Here, using combination of ChIP-seq, ChIP-proteomics, RNA-seq experiments embryonic stem cells where Atad2 is normally highly expressed, we found that an abundant nucleosome-bound protein present on active genes, associated with chromatin remodelling, DNA replication, repair factors. A structural analysis...
Genetic engineering is an important tool for redirecting the function of various types immune cells and their use therapeutic purpose. Although NK have many beneficial features, genetic targeted therapy focuses mostly on T cells. One major obstacles cell immunotherapy lack efficient method gene transfer. Lentiviral vectors been proven to be a safe engineering, however lentiviral transduction inefficient We show in this study that pseudotyped with modified baboon envelope glycoprotein can...