A5019335315- Immune cells in cancer
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- Phagocytosis and Immune Regulation
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Chemokine receptors and signaling
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Adipokines, Inflammation, and Metabolic Diseases
- Immunotherapy and Immune Responses
- Chronic Lymphocytic Leukemia Research
- Optimism, Hope, and Well-being
- COVID-19 Clinical Research Studies
- Ion channel regulation and function
- Cholesterol and Lipid Metabolism
- SARS-CoV-2 and COVID-19 Research
- Atherosclerosis and Cardiovascular Diseases
- Neuroscience and Neuropharmacology Research
- Inflammasome and immune disorders
- Single-cell and spatial transcriptomics
- TGF-β signaling in diseases
- Adipose Tissue and Metabolism
- Cancer Cells and Metastasis
- Pancreatic function and diabetes
Centre Méditerranéen de Médecine Moléculaire
2015-2024
Université Côte d'Azur
2018-2024
Centre National de la Recherche Scientifique
1996-2024
Laboratoire de PhysioMédecine Moléculaire
2021-2024
Inserm
1998-2022
Universidade Federal de Minas Gerais
2021
Creative Commons
2020
Fédération Hospitalo-Universitaire, Paris Center for Microbiome Medicine
2018-2019
Northwestern University
2016
Centre d’Immunologie de Marseille-Luminy
1996-2012
Cross-linking of the antigen receptor on lymphocytes by antigens or antibodies to results in activation enzymes protein kinase C (PKC) family. Mice homozygous for a targeted disruption gene encoding PKC-βI and PKC-βII isoforms develop an immunodeficiency characterized impaired humoral immune responses reduced cellular B cells, which is similar X-linked mice. Thus play important role cell may be functionally linked Bruton's tyrosine receptor-mediated signal transduction.
Autoantibodies and immune complexes are major pathogenic factors in autoimmune injury, responsible for initiation of the inflammatory cascade its resulting tissue damage. This activation results from interaction immunoglobulin (Ig)G Fc receptors containing an motif (ITAM) with (ICs) cytotoxic autoantibodies which initiates propagates response. In vitro, this pathway can be interrupted by coligation to FcγRIIB, IgG receptor inhibitory (ITIM). report, we describe vivo consequences FcγRII...
The marginal zone of the spleen is a precisely ordered region that contains specialized subsets B lymphocytes and macrophages. Disruption negative signaling inositol phosphatase, SH2-containing inositol-5-phosphatase 1 (SHIP), results in loss cells (MZBs) with reorganization macrophages (MZMOs) to red pulp spleen. This primary macrophage defect, as revealed by selectively depleting SHIP myeloid shows MZMOs are specifically required for retention MZBs. MZMO phenotype was reverted...
Macrophages face a substantial amount of cholesterol after the ingestion apoptotic cells, and LIPA (lysosomal acid lipase) has major role in hydrolyzing cholesteryl esters endocytic compartment.Here, we directly investigated LIPA-mediated clearance cells both vitro vivo.We show that inhibition causes defective efferocytic response because impaired generation 25-hydroxycholesterol 27-hydroxycholesterol. Reduced synthesis contributed to mitochondria-associated membrane leading mitochondrial...
Inflamed atherosclerotic plaques can be visualized by noninvasive positron emission and computed tomographic imaging with (18)F-fluorodeoxyglucose, a glucose analog, but the underlying mechanisms are poorly understood.Here, we directly investigated role of Glut1-mediated uptake in apolipoprotein E-deficient (ApoE(-/-)) mouse model atherosclerosis.We first showed that enhanced glycolytic flux atheromatous ApoE(-/-) mice was associated metabolic activity hematopoietic stem multipotential...
Along with respiratory tract disease per se , viral infections can also cause extrapulmonary complications a potentially critical impact on health. In the present study, we used an experimental model of influenza A virus (IAV) infection to investigate nature and outcome associated gut disorders.
Despite the ubiquitous function of macrophages across body, diversity, origin, and adrenal gland remain largely unknown. We define heterogeneity immune cells using single-cell RNA sequencing use genetic models to explore developmental mechanisms yielding macrophage diversity. populations monocyte-derived embryonically seeded identify a female-specific subset with low major histocompatibility complex (MHC) class II expression. In adulthood, monocyte recruitment dominates maintenance in female...
The Wiskott‐Aldrich syndrome (WAS) is a rare immunodeficiency disease affecting mainly platelets and lymphocytes. Here, we show that the WAS gene product, WASp, tyrosine phosphorylated upon aggregation of high affinity IgE receptor (FcϵRI) at surface RBL‐2H3 rat tumor mast cells. Lyn Bruton's kinase (Btk), two protein kinases involved in FcϵRI‐signaling phosphorylate WASp interact with vivo. Interestingly, expression GTPase defective mutant form CDC42, interacts accompanied by substantial...
Abstract Monocytes are part of the mononuclear phagocytic system. play a central role during inflammatory conditions and better understanding their dynamics might open therapeutic opportunities. In present study, we focused on characterization impact monocytes brown adipose tissue (BAT) functions remodeling. Single-cell RNA sequencing analysis BAT immune cells uncovered large diversity in monocyte macrophage populations. Fate-mapping experiments demonstrated that pool requires constant...
The entry of B lymphocytes into secondary lymphoid organs is a critical step in the development an immune response, providing site for repertoire shaping, antigen-induced activation and selection. These events are controlled by signals generated through cell antigen receptor (BCR) associated with changes migration properties cells response to chemokine gradients. stromal cell–derived factor (SDF)-1α thought be one driving forces during those processes, as it produced inside induces...
Thymic dendritic cells (DC) and epithelial play a major role in central tolerance but their respective roles are still controversial. Epithelial have the unique ability to ectopically express peripheral tissue-restricted antigens conferring self-tolerance tissues. Paradoxically, while negative selection seems occur for some of these antigens, cells, contrary DC, poor selectors. Using thymic cell line, we show functional intercellular transfer membrane material, including MHC molecules,...
Bruton tyrosine kinase (BTK) is a cytoplasmic protein which controls crucial steps of differentiation B lymphocytes. Mutations affecting either the PH, SH3, SH2 or domain BTK all give rise to X linked agammaglobulinaemia (XLA) in humans. In this study, authors report that BTK‐SH3 binds set proteins expressed pro‐B, pre‐B and cell lines. Three them were characterized as Vav, Sam68 EWS. The show Pro → Leu substitution region SH3 domain, deleted an XLA patient, sufficient abolish binding...
Enhanced glucose utilization can be visualized in atherosclerotic lesions and may reflect a high glycolytic rate lesional macrophages, but its causative role plaque progression remains unclear. We observe that the activity of carbohydrate-responsive element binding protein ChREBP is rapidly downregulated upon TLR4 activation macrophages. inactivation refocuses cellular metabolism to redox state favoring enhanced inflammatory responses after increased cell death or oxidized LDL loading....
1. Chloride channels were identified in the basolateral membrane of isolated cortical thick ascending limbs (CTALs) mouse nephron by patch‐clamp technique. A channel with a conductance 45 pS, previously shown to be Cl‐ selective, was detected 21% cell‐attached patches when CTAL fragments pre‐incubated 10 mumol l‐4 forskolin for at least 15 min. The same found only 8.5% formed on unstimulated tubules. 2. Another smaller (7‐9 pS) 42.8% and 57% inside‐out tubules, but 82‐87% from...
We compared gene expression signatures of aggressive amelanotic (Amela) melanomas with those slowly growing pigmented (Mela), identifying pathways potentially responsible for the Amela phenotype. Both tumors develop in mice upon conditional deletion melanocytes Ink4a/Arf tumor suppressor genes concomitant oncogene H-RasG12V and a known antigen. previously showed that only were highly infiltrated by leukocytes local systemic inflammation. report present pattern de-differentiation reduced...
Abstract TGF-β family cytokines play multiple roles in immune responses. TGF-β1-null mice suffer from multi-organ infiltration that leads to their premature death. T cells a central role the TGF-β1 phenotype, as deficiency of only reproduces lethal phenotype. Although it is known controls B isotype switch and homeostasis, source responsible for this control has not been characterized. Because major regulating cell responses, we addressed dependency cells. The analysis cell-deficient,...
Abstract Macrophages play a central role during infection, inflammation and tissue homeostasis maintenance. have been identified in all organs their core transcriptomic signature functions differ from one to another. Interestingly, macrophages also the peritoneal cavity these cells extensively used as model for phagocytosis, efferocytosis polarization. Peritoneal are involved B‐cell IgA production, control of wound healing following thermal‐induced liver surface injury. These presumably...
Defective cholesterol efflux pathways in mice promote the expansion of hematopoietic stem and progenitor cells a bias toward myeloid lineage, as observed chronic myelomonocytic leukemia (CMML). Here, we identify 5 somatic missense mutations ABCA1 26 patients with CMML. These confer proliferative advantage to monocytic cell lines vitro. In vivo inactivation or expression mutants setting Tet2 loss demonstrates myelosuppressive function ABCA1. Mechanistically, impair tumor-suppressor functions...