A5010610120- Immune Response and Inflammation
- S100 Proteins and Annexins
- Influenza Virus Research Studies
- Neonatal Respiratory Health Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Asthma and respiratory diseases
- Respiratory viral infections research
- Gut microbiota and health
- Inflammasome and immune disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Pediatric health and respiratory diseases
- SARS-CoV-2 and COVID-19 Research
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- COVID-19 Clinical Research Studies
- Phosphodiesterase function and regulation
- Immune Cell Function and Interaction
- Complement system in diseases
- Clostridium difficile and Clostridium perfringens research
- interferon and immune responses
- Protease and Inhibitor Mechanisms
- Biomarkers in Disease Mechanisms
- Pneumonia and Respiratory Infections
- Medical and Biological Ozone Research
- Probiotics and Fermented Foods
Brigham and Women's Hospital
2018-2025
Harvard University
2018-2025
Universidade Federal de Minas Gerais
2014-2023
Pulmonary and Critical Care Associates
2023
Centre Méditerranéen de Médecine Moléculaire
2021
Inserm
2021
Université Côte d'Azur
2021
International Union of Basic and Clinical Pharmacology
2020
Faculdade de Ciências Médicas de Minas Gerais
2020
Secondary bacterial infections often complicate viral respiratory infections. We hypothesize that perturbation of the gut microbiota during influenza A virus (IAV) infection might favor superinfection. Sublethal with transiently alters composition and fermentative activity in mice. These changes are attributed part to reduced food consumption. Fecal transfer experiments demonstrate IAV-conditioned compromises lung defenses against pneumococcal infection. In mechanistic terms, production...
Neutrophil cytoplasts promote T H 17 inflammation.
This study aimed at assessing whether AnxA1, a downstream mediator for the anti-inflammatory effects of GCs, could affect fate immune cells in tissue exudates, using LPS-induced pleurisy BALB/c mice. AnxA1 protein expression exudates was increased during natural resolution, as seen 48-72 h post-LPS, an effect augmented by treatment with GC and associated marked presence apoptotic neutrophils pleural exudates. The functional relevance determined neutralizing antibody or nonspecific antagonist...
Gout is a disease characterized by the deposition of monosodium urate (MSU) crystals in joints. Continuous gout episodes may lead to unresolved inflammatory responses and tissue damage. We investigated effects high-fiber diet acetate, short-chain fatty acid (SCFA) resulting from metabolism fiber gut microbiota, on response an experimental model mice. Injection MSU into knee joint mice induced neutrophil influx hypernociception. The onset was not altered animals given diet, but faster...
Klebsiella pneumoniae (Kp) a common cause of pneumonia leads to intense lung injury and mortality that are correlated with infective exacerbations. Probiotics class microorganisms have immunomodulatory effects benefit health. We investigated whether the probiotic Bifidobacterium longum 5(1A) induces protection in mice against infection induced by Kp potential involved mechanisms. secretion pro-inflammatory cytokines, neutrophil recruitment, significant bacterial load 50% lethality. However,...
Pneumonia is one of the leading causes death and mortality worldwide. The inflammatory responses that follow respiratory infections are protective to pathogen clearance but can also be deleterious if unregulated. microbiota known an important barrier against infections, mediating both direct inhibitory effects potential regulating immune contributing a proper return homeostasis. GPR43 receptor for acetate, metabolite shown induce regulate functions. Here we addressed role signaling during...
Along with respiratory tract disease per se , viral infections can also cause extrapulmonary complications a potentially critical impact on health. In the present study, we used an experimental model of influenza A virus (IAV) infection to investigate nature and outcome associated gut disorders.
The resolution of infection is an active process with specific molecular and cellular mechanisms that temper inflammation enhance pathogen clearance. Here, the specialized pro-resolving mediator (SPM) Maresin 1 (MaR1) inhibited respiratory syncytial virus (RSV)-induced inflammation. inlerleukin-13 production from type 2 innate lymphoid cells (ILC) CD4 T helper was decreased by exogenous MaR1. In addition, MaR1 increased amphiregulin RSV viral transcripts to promote resolution. also promoted...
Abstract PDE4 inhibition is effective at resolving neutrophilic inflammation after LPS-challenge by promoting caspase-3-dependent apoptosis of inflammatory cells in the pleural cavity. inhibitors are anti-inflammatory drugs whose effects and putative mechanisms on resolution neutrophil vivo still unclear. Here, we examined specific cavity LPS-challenged mice. LPS induced recruitment that was increased 4 h, peaked 8–24 declined thereafter. Such an event preceded levels KC MIP-2 1 2 h....
Influenza virus A (IAV) causes annual epidemics and intermittent pandemics that affect millions of people worldwide. Potent inflammatory responses are commonly associated with severe cases IAV infection. The complement system, an important mechanism innate humoral immune to infections, is activated during primary infection mediates, in association natural IgM, viral neutralization by virion aggregation coating hemmagglutinin. Increased levels the anaphylatoxin C5a were found patients fatally...
Glucocorticoid (GC)-induced leucine zipper (GILZ) has been shown to mediate or mimic several actions of GC. This study assessed the role GILZ in self-resolving and GC-induced resolution neutrophilic inflammation induced by LPS mice. expression was increased during phase LPS-induced pleurisy, especially macrophages with resolving phenotypes. Pretreating LPS-injected mice trans-activator transcription peptide (TAT)-GILZ, a cell-permeable fusion protein, shortened intervals improved indices....
Streptococcus pneumoniae is a major cause of community-acquired pneumonia leading to high mortality rates. Inflammation triggered by pneumococcal infection necessary for bacterial clearance but must be spatially and temporally regulated prevent further tissue damage dissemination. Annexin A1 (AnxA1) mainly acts through Formyl Peptide Receptor 2 (FPR2) inducing the resolution inflammation. Here, we have evaluated role AnxA1 FPR2 during in mice. For that, AnxA1, Fpr2/3 knockout (KO) mice...
Sepsis is a lethal syndrome characterized by systemic inflammation and abnormal coagulation. Despite therapeutic advances, sepsis mortality remains substantially high. Herein, we investigated the role of plasminogen/plasmin (Plg/Pla) system during sepsis. Plasma levels Plg were significantly lower in mice subjected to severe compared with nonsevere sepsis, whereas IL-6, marker severity, higher correlated negatively IL-6 both septic patients, plasminogen activator inhibitor-1 positively IL-6....
Influenza A virus causes annual epidemics which affect millions of people worldwide. recent pandemic brought new awareness over the health impact disease. It is thought that a severe inflammatory response against contributes to disease severity and death. Therefore, modulating effects mediators may represent therapy infection. Platelet activating factor (PAF) receptor (PAFR) deficient mice were used evaluate role gene in model experimental infection with A/WSN/33 H1N1 or reassortant H3N1...
Gout is a self‐limited inflammatory disease caused by deposition of monosodium urate (MSU) crystals in the joints. Resolution inflammation an active process leading to restoration tissue homeostasis. Here, we studied role Annexin A1 (AnxA1), glucocorticoid‐regulated protein that has anti‐inflammatory and proresolving actions, resolution acute gouty inflammation. Injection MSU knee joint mice induced was associated with expression AnxA1 during resolving phase Neutralization antiserum or...
Rationale: Influenza A infections are a leading cause of morbidity and mortality worldwide especially when associated with secondary pneumococcal infections. Inflammation is important to control pathogen proliferation but may also tissue injury death. CXCR1/2 chemokine receptors relevant for the recruitment neutrophils. We investigated role during influenza, post-influenza Methods: Mice were infected influenza virus (IAV) or Streptococcus pneumoniae then treated daily antagonist DF2162. To...
The plasminogen (Plg)/plasmin (Pla) system is associated with a variety of biological activities beyond the classical dissolution fibrin clots, including cell migration, tissue repair, and inflammation. Although capacity Plg/Pla to induce migration well defined, mechanism underlying this process in vivo elusive. In study, we show that Pla induces vitro murine fibroblasts macrophages (RAW 264.7) dependent on MEK/ERK pathway by requiring its proteolytic activity lysine binding sites. Plasmin...
Abstract Annexin A1 (AnxA1) is a glucocorticoid-regulated protein endowed with anti-inflammatory and proresolving properties. Intact AnxA1 37-kDa that may be cleaved in vivo at the N-terminal region by neutrophil proteases including elastase proteinase-3, generating 33-kDa isoform largely inactive. In this study, we investigated dynamics of expression effects synthetic (sivelestat [SIV]; Eglin) natural (secretory leukocyte protease inhibitor [SLPI]; Elafin) inhibitors on resolution...
The innate immune system is the first line of host defense against invading organisms. Thus, pathogens have developed virulence mechanisms to evade system. Here, we report a novel means for inhibition neutrophil recruitment by Group A Streptococcus (GAS). Deletion secreted esterase gene (designated sse) in M1T1 GAS strains with (MGAS5005) and without (MGAS2221) null covS mutation enhances ingress infection sites skin mice. In trans expression SsE MGAS2221 reduces invasion. sse deletion...
Pneumococcal pneumonia is a leading cause of mortality worldwide. The inflammatory response to bacteria necessary control infection, but it may also contribute tissue damage. Phosphodiesterase-4 inhibitors, such as rolipram (ROL), effectively reduce inflammation. Here, we examined the impact ROL in pneumococcal murine model. Mice were infected intranasally with 10(5)-10(6) CFU Streptococcus pneumoniae, treated prophylactic or therapeutic schedule combination, not, antibiotic ceftriaxone....
Nonphlogistic migration of macrophages contributes to the clearance pathogens and apoptotic cells, a critical step for resolution inflammation return homeostasis. Angiotensin-(1-7) [Ang-(1-7)] is heptapeptide renin-angiotensin system that acts through Mas receptor (MasR). Ang-(1-7) has recently emerged as novel proresolving mediator, yet mechanisms are not fully determined. Herein, stimulated human murine monocytes/macrophages in MasR-, CCR2-, MEK/ERK1/2-dependent manner. Pleural injection...