A5025411518- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Immune cells in cancer
- Drug-Induced Hepatotoxicity and Protection
- Liver Disease Diagnosis and Treatment
- Research on Leishmaniasis Studies
- Mast cells and histamine
- Sarcoma Diagnosis and Treatment
- Liver Disease and Transplantation
- IL-33, ST2, and ILC Pathways
- Neonatal Respiratory Health Research
- Urticaria and Related Conditions
- Macrophage Migration Inhibitory Factor
- Cytokine Signaling Pathways and Interactions
- Pancreatic function and diabetes
- Diabetic Foot Ulcer Assessment and Management
- Photodynamic Therapy Research Studies
- Click Chemistry and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Trypanosoma species research and implications
- Essential Oils and Antimicrobial Activity
- Vagus Nerve Stimulation Research
- Liver physiology and pathology
- Thermal Regulation in Medicine
- Organ Transplantation Techniques and Outcomes
University of Calgary
2017-2025
Ferrari (Italy)
2023
International Network for Cancer Treatment and Research
2022
Health Foundation
2022
BioClinica (United States)
2022
University of Aveiro
2017-2021
Universidade Federal de Minas Gerais
2013-2019
Universidade de São Paulo
2017
Fundação Oswaldo Cruz
2012
Kupffer cells (KCs) are localized in liver sinusoids but extend pseudopods to parenchymal maintain their identity and serve as the body's central bacterial filter. Liver cirrhosis drastically alters vascular architecture, how KCs adapt is unclear. We used a mouse model of fibrosis human tissue examine immune adaptation. Fibrosis forced lose contact with cells, down-regulating "KC identity," which rendered them incapable clearing bacteria. Commensals stimulated recruitment monocytes through...
Drug‐induced liver injury (DILI) is an important cause of acute failure, with limited therapeutic options. During DILI, oncotic necrosis concomitant release and recognition intracellular content amplifies inflammation injury. Among these molecules, self‐DNA has been widely shown to trigger inflammatory autoimmune diseases; however, whether DNA released from damaged hepatocytes accumulates into necrotic the impact its by immune system remains elusive. Here we show that treatment two different...
Every day, megakaryocytes produce billions of platelets that circulate for several days and eventually are cleared by the liver. The exact removal mechanism, however, remains unclear. Loss sialic acid residues is thought to feature in aging clearance platelets. Using state-of-the-art spinning disk intravital microscopy delineate different compartments cells mouse liver, we observed rapid accumulation desialylated predominantly on Kupffer cells, with only a few endothelial none hepatocytes....
γδ T cells have many known functions, including the regulation of antibody responses. However, how control humoral immunity remains elusive. Here we show that complete Freund's adjuvant (CFA), but not alum, immunization induces a subpopulation CXCR5-expressing in draining lymph nodes. TCRγδ+CXCR5+ present antigens to, and induce CXCR5 on, CD4 by releasing Wnt ligands to initiate follicular helper (Tfh) cell program. Accordingly, TCRδ-/- mice impaired germinal center formation, inefficient...
The aim of this work is to assess the potential curcumin in photosensitization biofilms Listeria. Biofilms Listeria innocua, were irradiated with blue light presence a curcumin‐rich extract Curcuma longa or commercial curcumin. Similar experiments conducted planktonic cells, for comparison. A reduction 4·9 log concentration viable biofilm cells was obtained 3·7 mg l−1 Planktonic much more susceptible (6·1 reduction). tetracationic porphyrin, used as reference photosensitizer (PS), caused...
Cocaine is a commonly abused illicit drug that causes significant morbidity and mortality. The most severe common complications are seizures, ischemic strokes, myocardial infarction, acute liver injury. Here, we demonstrated cocaine intoxication promoted seizure along with damage in mice, intense inflammatory infiltrate. Considering the protective role of endocannabinoid system against cell toxicity, hypothesized treatment an anandamide hydrolysis inhibitor, URB597, or phytocannabinoid,...
Acetaminophen (APAP) is usually safe when administrated in therapeutic doses; however, APAP overdose can lead to severe liver injury. cause direct hepatocyte damage, and stimulates an inflammatory response leading oxidative stress. Supressor of Cytokine Signaling (SOCS) 2 modulates cytokine growth factor signaling, plays a role the regulation hepatic cellular processes. Our study evaluated SOCS2 The administration toxic dose (600 mg/kg) caused significant necrosis WT mice. In SOCS2-/- mice,...
The gastrointestinal (GI) tract harbors commensal microorganisms as well invasive bacteria, toxins and other pathogens and, therefore, plays a pivotal barrier immunological role against pathogenic agents. vagus nerve is an important regulator of the GI tract-associated immune system, having profound effects on inflammatory responses. Among organs, liver key site surveillance, it has large population resident macrophages receives blood drained from guts through hepatic portal circulation....
Abstract It is becoming clear that every organ seeded by a population of fetal liver–derived macrophages are replaced at different rates monocyte-derived macrophages. Using the Ms4a3tdTomato reporter mouse reports on alveolar (Mo-AMs) and our ability to examine AM function using multichannel intravital microscopy, we examined fetal-liver derived macrophage (FL-AM) Mo-AM populations within same under various environmental conditions. The experiments unveiled AMs migrated from alveolus...
Sandflies are vectors of Leishmania, the causative agent leishmaniasis in mammalian hosts, including humans. The protozoan parasite is transmitted by sandfly bite during salivation that occurs at moment blood feeding. components vector saliva include anticlotting and vasodilatory factors facilitate flow immunomodulatory inhibit wound healing quell immune response. Not surprisingly, these also play important roles establishment Leishmania infection. To date, majority knowledge has been...
Pneumococcal pneumonia is a leading cause of mortality worldwide. The inflammatory response to bacteria necessary control infection, but it may also contribute tissue damage. Phosphodiesterase-4 inhibitors, such as rolipram (ROL), effectively reduce inflammation. Here, we examined the impact ROL in pneumococcal murine model. Mice were infected intranasally with 10(5)-10(6) CFU Streptococcus pneumoniae, treated prophylactic or therapeutic schedule combination, not, antibiotic ceftriaxone....
Hepatocytes may rupture after a drug overdose, and their intracellular contents act as damage-associated molecular patterns (DAMPs) that lead to additional leukocyte infiltration, amplifying the original injury. Necrosis-derived DNA can be recognized DAMP, activating liver non-parenchymal cells (NPCs). We hypothesized NPCs react by releasing interferon (IFN)-1, which amplifies acetaminophen (APAP)-triggered necrosis. orally overdosed different knockout mouse strains investigate pathways...
Abstract Severe acute respiratory syndrome coronavirus–2 (SARS-CoV-2) enters the tract, where it infects alveoli epithelial lining. However, patients have sequelae that extend well beyond into pulmonary vasculature and, perhaps, to brain and other organs. Because of dynamic events within blood vessels, histology does not report platelet neutrophil behavior. rapid nontranscriptional response these cells, neither single-cell RNA sequencing nor proteomics robustly on their critical behaviors....
In adults, liver-resident macrophages, or Kupffer cells (KCs), reside in the sinusoids and sterilize circulating blood by capturing rapidly flowing microbes. We developed quantitative intravital imaging of 1-day-old mice combined with transcriptomics, genetic manipulation, vivo infection assays to interrogate increased susceptibility newborns bloodstream infections. Whereas KCs were better at catching
Objective: To investigate the hepatoprotective effects of mitochondrial transplantation in a murine liver ischemia/reperfusion (I/R) model. Summary Background Data: Sequential ischemia followed by reperfusion is pathophysiological process underlying hepatocellular injury number clinical contexts, such as hemorrhagic shock/resuscitation, major elective surgery and organ transplantation. A unifying pathogenic consequence I/R dysfunction. Restoration mitochondria via (MTx) has emerged potential...
Mechanism of encapsulated bacteria recognition and capture by Kupffer cells.