A5069286971- Gut microbiota and health
- Gastrointestinal motility and disorders
- Tryptophan and brain disorders
- Tea Polyphenols and Effects
- Diet and metabolism studies
- Clostridium difficile and Clostridium perfringens research
- Inflammatory Bowel Disease
- Probiotics and Fermented Foods
- Microscopic Colitis
- Nutritional Studies and Diet
- Health, psychology, and well-being
- Helicobacter pylori-related gastroenterology studies
- Escherichia coli research studies
- Immune Cell Function and Interaction
- Dietary Effects on Health
- Dermatology and Skin Diseases
- IL-33, ST2, and ILC Pathways
- Enterobacteriaceae and Cronobacter Research
- Misinformation and Its Impacts
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood Coagulation and Thrombosis Mechanisms
- Ion Transport and Channel Regulation
- Educational Strategies and Epistemologies
- Ion Channels and Receptors
- Pancreatitis Pathology and Treatment
University of Calgary
2009-2024
University of Florida
2014-2022
Florida College
2018
University of Arizona
2018
University of Alberta
2013
Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer death in United States yet data are scant regarding host factors influencing pancreatic carcinogenesis. Increasing evidence support role microbiota carcinogenesis but its PDAC not well established. Herein, we report that antibiotic-mediated microbial depletion KrasG12D/PTENlox/+ mice showed a decreased proportion poorly differentiated tumors compared to microbiota-intact mice. Subsequent 16S rRNA PCR ~50% with...
Kupffer cells (KCs) are localized in liver sinusoids but extend pseudopods to parenchymal maintain their identity and serve as the body's central bacterial filter. Liver cirrhosis drastically alters vascular architecture, how KCs adapt is unclear. We used a mouse model of fibrosis human tissue examine immune adaptation. Fibrosis forced lose contact with cells, down-regulating "KC identity," which rendered them incapable clearing bacteria. Commensals stimulated recruitment monocytes through...
The concept that the intestinal microbiota modulates numerous physiologic processes, including immune development and function, nutrition metabolism, pathogen exclusion, is relatively well established in scientific community. molecular mechanisms driving these various effects events leading to establishment of a "healthy" microbiome are slowly emerging. This review brings into focus important aspects microbial/host interactions intestine discusses key controlling health disease states. We...
Intestinal tuft cells are a rare, poorly understood cell type recently shown to be critical mediator of 2 immune response helminth infection. Here, we present advances in segmentation algorithms and analytical tools for multiplex immunofluorescence (MxIF), platform that enables iterative staining over 60 antibodies on single tissue section. These refinements have enabled comprehensive analysis number, distribution, protein expression profiles as function anatomical location physiological...
Abstract Background Studies on the inhibition of inflammation by infection with helminth parasites have, until recently, overlooked a key determinant health: gut microbiota. Infection helminths evokes changes in composition their host’s microbiota: one outcome which is an altered metabolome (e.g., levels short-chain fatty acids (SCFAs)) lumen. The functional implications helminth-evoked enteric microbiome (composition and metabolites) are poorly understood explored respect to controlling...
Despite achieving endoscopic remission, over 20% of inflammatory bowel disease (IBD) patients experience chronic abdominal pain. Visceral pain and the microbiome exhibit sex-dependent interactions, while visceral in IBD shows a sex bias. Our aim was to evaluate whether post-inflammatory microbial perturbations contribute hypersensitivity manner.
The gut microbiota plays a role in shaping overall host health and response to several cancer treatments. Factors, such as diet, exercise, chemotherapy, can alter the microbiota. In present study, Alberta Cancer Exercise (ACE) program was investigated strategy favorably modify of breast survivors who had received chemotherapy. Subsequently, ability post-exercise microbiota, alone or with prebiotic fiber supplementation, influence outcomes interrogated using fecal transplant (FMT) germ-free...
Thearubigins, polymers of tea catechins, account for more than 20% the black polyphenols and have been reported to be active components in tea. However, chemical structures underlying mechanisms regarding how thearubigins, being poorly bioavailable, generate vivo health benefits are still largely unknown. Using germ-free specific pathogen-free husbandry conditions combined with LC/MS-based nontargeted targeted metabolomic analyses, we investigated role intestinal bacteria thearubigin...
Diet influences the pathogenesis and clinical course of inflammatory bowel disease (IBD). The Mediterranean diet (MD) is linked to reductions in biomarkers alterations microbial taxa metabolites associated with health. We aimed identify features gut microbiome that mediate relationship between MD fecal calprotectin (FCP) ulcerative colitis (UC). Weighted gene co-expression network analysis (WGCNA) was used modules co-abundant correlated FCP. considered were taxa, serum metabolites, dietary...
Scope This study is to determine the in vivo efficacy of black tea theaflavin (TF) detoxify two metabolic toxins, ammonia and methylglyoxal (MGO), mice Methods results Under vitro conditions, TF able react with ammonia, MGO, hydrogen peroxide produce its aminated, MGO conjugated, oxidized products, respectively. In TF‐treated mice, aminated TF, conjugates are searched using LC‐MS/MS. The provide first evidence that unabsorbed trap form TF; furthermore, both have capacity generate...
Western-style diets high in saturated fat and refined carbohydrate have been shown to alter gut microbiota as well being associated with altered behaviour learning ability. The objective of this study was determine the effects short-term intake a diet on intestinal cytokine expression, tryptophan metabolism, levels neurotransmitters brain.At 7 weeks age, 129S1/SvImJ mice were placed standard chow or (fat 33%, carbohydrates 49%) for 3 weeks. Anxiety-like assessed by latency step-down test...
Proteinase-activated receptor (PAR)<sub>2</sub> is activated by trypsin-like serine proteinases and has been implicated in intestinal inflammation. However, its role the regulation of mucosal function remains unclear. Using epithelial cell line, SCBN, we have studied stimulus-secretion coupling mechanisms PAR<sub>2</sub>-induced chloride transport, focusing on cyclooxygenase (COX)-1 COX-2 activities prostaglandin (PG) E<sub>2</sub> secretion. SCBN monolayers were grown Snapwell supports,...
Increasing evidence has shown that gut microbiota plays important roles in metabolizing large molecular polyphenols to bioavailable and bioactive microbial metabolites. Theaflavin (TF) is one of the major color compounds black tea demonstrated anti-inflammation, antioxidant, anticancer effects properties. However, little known about metabolism TF by vivo. In this study, following administration mice, C-ring cleavage metabolites, dihydro- tetrahydro-theaflavin (DH-TF TH-TF) were detected...
Abstract Antigen (Ag)-specific tolerization prevents type 1 diabetes (T1D) in non-obese diabetic (NOD) mice but proved less effective humans. Several auto-Ags are fundamental to disease development, suggesting T1D etiology is heterogeneous and may limit the effectiveness of Ag-specific therapies distinct endotypes. Colonization factor antigen I (CFA/I) fimbriae from Escherichia coli can inhibit autoimmune diseases murine models by inducing bystander tolerance. To test if Ag-independent...
How the gut microbiota and immune system maintain intestinal homeostasis in concert with enteric nervous (ENS) remains incompletely understood. To address this gap, we assessed small transit, neuronal density, neurogenesis, microbiota, cell populations cytokines wildtype T-cell deficient germ-free mice colonized specific pathogen-free (SPF) conventionally raised SPF segmented filamentous bacteria (SFB)-monocolonized mice. increased transit a T cell-dependent manner. density myenteric...
Both pathogenic and commensal strains of Escherichia coli colonize the human intestinal tract. Pathogenic differ only in expression virulence factors, many which comprise a type III secretion system (TTSS). Little is known regarding effect E. on epithelial response to secretagogues that drive ion secretion, despite its importance causing clinically significant diarrhoea. Using Ussing chambers measure electrogenic transport T84 cell monolayers, we found all tested (pathogenic, commensal,...