A5103027115- Parasites and Host Interactions
- Gut microbiota and health
- Parasite Biology and Host Interactions
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Helminth infection and control
- Head and Neck Surgical Oncology
- Inflammatory Bowel Disease
- Helicobacter pylori-related gastroenterology studies
- Vascular Malformations Diagnosis and Treatment
- Intracranial Aneurysms: Treatment and Complications
- Brain Metastases and Treatment
- Meningioma and schwannoma management
- Escherichia coli research studies
- Immune cells in cancer
- Reproductive System and Pregnancy
- Cerebrospinal fluid and hydrocephalus
- Immune Cell Function and Interaction
- Parasitic infections in humans and animals
- Barrier Structure and Function Studies
- Microscopic Colitis
- Metabolomics and Mass Spectrometry Studies
- Tea Polyphenols and Effects
- ATP Synthase and ATPases Research
- Neurofibromatosis and Schwannoma Cases
University of Calgary
2015-2024
Tulane Medical Center
2023
New York Medical College
2015-2018
Westchester Medical Center
2015-2018
Neurological Surgery
2016-2018
Institute of Infection and Immunity
2010-2011
Rainbow Babies & Children's Hospital
2010
Case Western Reserve University
2010
The Neurological Institute
2010
Canadian Society of Intestinal Research
2010
Abstract There is increasing evidence that parasitic helminth infection has the ability to ameliorate other disease conditions. In this study of rat tapeworm, Hymenolepis diminuta, modulate dinitrobenzene sulfonic acid (DNBS)-induced colitis in mice assessed. Mice receiving DNBS (3 mg intrarectally) developed by 72 h after treatment. infected 8 days before with five H. diminuta larvae were significantly protected from colitis, as gauged reduced clinical disease, histological damage scores,...
Human IL-4 macrophages promote epithelial wound recovery and suppress colitis, supporting their use as a cell therapy for IBD.
Altered intestinal barrier function has been implicated in the pathophysiology of ulcerative colitis (UC) genetic, functional, and epidemiological studies. Mast cells corticotropin-releasing factor (CRF) regulate mucosal human colon. Because eosinophils are often increased colon tissues patients with UC, we assessed interactions among mast cells, CRF, these patients.Transmucosal fluxes protein antigens (horseradish peroxidase) paracellular markers ((51)Cr-EDTA, fluorescein...
Abstract Background Studies on the inhibition of inflammation by infection with helminth parasites have, until recently, overlooked a key determinant health: gut microbiota. Infection helminths evokes changes in composition their host’s microbiota: one outcome which is an altered metabolome (e.g., levels short-chain fatty acids (SCFAs)) lumen. The functional implications helminth-evoked enteric microbiome (composition and metabolites) are poorly understood explored respect to controlling...
A characteristic of many enteropathies is increased epithelial permeability, a potentially pathophysiological event that can be evoked by T helper (Th)-1 (i.e., IFN-gamma) and Th2 IL-4) cytokines bacterial infection [e.g., enteropathogenic Escherichia coli (EPEC)]. The green tea polyphenol (-)-epigallocatechin gallate (EGCG) has immunosuppressive properties, we hypothesized it would ameliorate the permeability induced IFN-gamma, IL-4, and/or EPEC. EGCG, but not related epigallocatechin,...
Helminth parasites provoke multicellular immune responses in their hosts that can suppress concomitant disease. The gut lumen-dwelling tapeworm Hymenolepis diminuta, unlike other assessed as helminth therapy, causes no host tissue damage while potently suppressing murine colitis. With the goal of harnessing immunomodulatory capacity infection with H. we putative generation anti-colitic regulatory B cells following diminuta infection. Splenic CD19(+) isolated from mice infected 7 [HdBc(7(d))]...
Alternatively activated macrophages (AAMs) (or M2a) can inhibit colitis but may also be associated with fibrosis. Thus, by using the dinitrobenzene sulfonic (DNBS) murine model of colitis, this study aimed to determine whether 1) bone marrow (BM)-derived AAMs could reduce 2) any anticolitic effect BM-AAMs was IL-10 dependent, and 3) repeated AAM treatments remained effective were fibrosis in gut or other tissues. Balb/c mice received (10 6 intraperitoneally) from wild-type (WT) −/− 48 h...
Interferon-γ (IFNγ) is an important immunoregulatory cytokine that can also decrease intestinal epithelial barrier function. Little known about the intracellular signalling events immediately subsequent to IFNγ/IFNγ receptor interaction mediate increases in permeability; data could be used ablate this effect of IFNγ while leaving its immunostimulatory effects intact. This study assessed potential involvement Src family kinases IFNγ-induced permeability using confluent filter-grown monolayers...
Background & AimsAdherent-invasive Escherichia coli are implicated in inflammatory bowel disease, and mitochondrial dysfunction has been observed biopsy specimens from patients with disease. As a novel aspect of adherent-invasive E coli–epithelial interaction, we hypothesized that (strain LF82) would elicit substantial disruption epithelial form function.MethodsMonolayers human colon-derived cell lines were exposed to coli–LF82 or commensal RNA sequence analysis, function (adenosine...
Purpose . To compare infection with the tapeworm, Hymenolepis diminuta , steroid (dexamethasone) administration in inhibition of dinitrobenzene sulfonic acid- (DNBS-) induced colitis mice. Procedures Mice were treated DNBS<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mo>±</mml:mo></mml:math>infected H. or daily dexamethasone (2 mg/Kg, ip.) and assessed 72 hours post-DNBS by calculation disease activity histological damage scores, spleen cell cytokine production. Results...
Interleukin (IL)-22, an immune cell-derived cytokine whose receptor expression is restricted to non-immune cells (e.g. epithelial cells), can be anti-inflammatory and pro-inflammatory. Mice infected with the tapeworm Hymenolepis diminuta are protected from dinitrobenzene sulphonic acid (DNBS)-induced colitis. Here we assessed expulsion of H. diminuta, concomitant response outcome DNBS-induced colitis in wild-type (WT) IL-22 deficient mice (IL-22-/-) ± infection. Interleukin-22-/- had a...
The epithelial lining of mucosal surfaces acts as a barrier to regulate the entry antigen and pathogens. Nowhere is this function contiguous epithelium more important than in gut, which continually exposed huge antigenic load and, colon, an immense commensal microbiota. We assessed intracellular signaling events that underlie interferon (IFN) γ-induced increases permeability using monolayers human colonic T84 cell line. Confluent on semipermeable supports were treated with IFNγ (20 ng/ml),...
Awareness of the immunological underpinnings host-parasite interactions may reveal immune signaling pathways that could be used to treat inflammatory disease in humans. Previously we showed infection with rat tapeworm, Hymenolepis diminuta, as a model helminth, or systemic delivery worm antigen (HdAg) significantly reduced severity dinitrobenzene sulfonic acid (DNBS)-induced colitis mice. Extending these analyses, intraperitoneal injection HdAg dose-dependently suppressed dextran sodium...
Gut bacteria provide benefits to the host and have been implicated in inflammatory bowel disease (IBD), where adherent-invasive E. coli (AIEC) pathobionts (e.g., strain LF82) are associated with Crohn's disease. coli-LF82 causes fragmentation of epithelial mitochondrial network, leading increased permeability. We hypothesized that butyrate would limit disruption caused by coli-LF82. Human colonic organoids T84 cell line infected (MOI = 100, 4 h) showed a significant increase network fission...
The barrier function of the epithelium lining intestine is essential for health by preventing free passage colonic bacteria into mucosa. Epithelia treated with interferon (IFN)-γ display increased transcytosis. Much known how IFNγ affects tight junction and paracellular permeability, yet its role in modifying transcellular traffic commensal remains poorly understood. Using immunoblotting, ELISA immunolocalization, was found to activate extracellular regulated kinase (ERK)1/2 human colon-like...
The enteric epithelium must absorb nutrients and water act as a barrier to the entry of luminal material into body; this function is key component innate immunity. Nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy occurs via inhibition prostaglandin synthesis perturbed epithelial mitochondrial activity. Here, direct effect NSAIDs [indomethacin, piroxicam (cyclooxygenase 1 2 inhibitors), SC-560 (a cyclooxygenase inhibitor)] on human T84 cell line monolayers was assessed by...
The adoptive transfer of alternatively activated macrophages (AAMs) has proven to attenuate inflammation in multiple mouse models colitis; however, the effect cryopreservation on AAMs, ability previously frozen AAMs block dinitrobenzene sulfonic acid (DNBS) (Th1) and oxazolone (Th2) colitis their migration postinjection remains unknown. Here we have found that while reduced mRNA expression canonical markers interleukin (IL)-4-treated [M(IL-4)], this step did not translate protein or...