A5066060676- Inflammatory Bowel Disease
- Microscopic Colitis
- Helicobacter pylori-related gastroenterology studies
- Reproductive System and Pregnancy
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Ion Transport and Channel Regulation
- Cell Adhesion Molecules Research
- Inflammatory mediators and NSAID effects
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood Coagulation and Thrombosis Mechanisms
- Ion channel regulation and function
- Parasites and Host Interactions
- Pregnancy and Medication Impact
- Digestive system and related health
- Vitamin D Research Studies
- Ion Channels and Receptors
- Phagocytosis and Immune Regulation
- Cytokine Signaling Pathways and Interactions
- Protease and Inhibitor Mechanisms
- Barrier Structure and Function Studies
- Whipple's Disease and Interleukins
- Psoriasis: Treatment and Pathogenesis
- Receptor Mechanisms and Signaling
- HER2/EGFR in Cancer Research
University of Calgary
2006-2017
Research Network (United States)
2009-2013
Open Geospatial Consortium
2013
Health Sciences Centre
2012
Inflammation Research Foundation
2009-2010
Institute of Infection and Immunity
2010
McMaster University
2005-2009
Universidad Nacional Autónoma de México
1999
IL-17 and Foxp3 double-expressing (DE) CD4(+) T lymphocytes are novel crossover immune cell population, but the presence role of these cells in human intestinal inflammation is unclear. The aim this study was to investigate circulating DE patients with inflammatory bowel disease (IBD).The entire cohort consisted 79 subjects: 31 Crohn's disease, 28 ulcerative colitis, 20 healthy control subjects (HC). IBD evidence active at endoscopy were entered into study. Peripheral blood mononuclear used...
Transient Receptor Potential Melastatin–8 (TRPM8), a recently identified member of the transient receptor potential (TRP) family ion channels, is activated by mild cooling and chemical compounds such as supercooling agent, icilin. Since cooling, possibly involving TRPM8 stimulation, diminishes injury-induced peripheral inflammation, we hypothesized that activation may also attenuate systemic inflammation. We thus studied involvement in regulating colonic inflammation using two mouse models...
The simplistically and ambiguously termed 'fibrostenosis' of bowel is a hallmark severe Crohn's disease [CD] major contributor to medical treatment failure. Non-invasive imaging assessment novel therapy targeting this condition are under investigation, which particularly requires better understanding the underlying histological basis.We analysed 48 patients with stricturing ileitis or/and colitis that required surgical resection. most representative sections fibrostenotic, non-stenotic...
Abstract There is increasing evidence that parasitic helminth infection has the ability to ameliorate other disease conditions. In this study of rat tapeworm, Hymenolepis diminuta, modulate dinitrobenzene sulfonic acid (DNBS)-induced colitis in mice assessed. Mice receiving DNBS (3 mg intrarectally) developed by 72 h after treatment. infected 8 days before with five H. diminuta larvae were significantly protected from colitis, as gauged reduced clinical disease, histological damage scores,...
Distinction between 2 forms of inflammatory bowel disease (IBD), ulcerative colitis (UC) and Crohn's (CD), can be challenging. Aberrant mucosal immunity suggests that CD is a T helper type 1 cell (Th1)-driven disease, whereas UC as Th2-driven response. However, whether this paradigm truly distinguishes from controversial. We aimed to clarify the discriminating potential lamina propria Th subsets in patients with IBD. Biopsies 79 IBD 20 healthy controls were collected for analysis...
Mucosal biopsies from inflamed colon of inflammatory bowel disease patients exhibit elevated epithelial apoptosis compared with those healthy individuals, disrupting mucosal homeostasis and perpetuating disease. Therapies that decrease intestinal may, therefore, ameliorate disease, but treatments specifically target apoptotic pathways are lacking. Proteinase-activated receptor-2 (PAR2), a G protein-coupled receptor activated by trypsin-like serine proteinases, is expressed on cells...
Background Cannabinoid type 1 (CB1) receptors are involved in the regulation of gastrointestinal (GI) motility and secretion. Our aim was to characterize roles CB1 receptor on GI secretion vitro vivo by using different classes antagonists. Methods Immunohistochemistry used examine localization mouse ileum colon. Organ bath experiments testing comprising upper transit, colonic expulsion, whole gut transit were performed effects inverse agonist/antagonist AM251 neutral antagonist AM4113. As a...
Background and Purpose The pathogenesis of the inflammatory bowel diseases (IBD), comprising Crohn's disease (CD) ulcerative colitis (UC), involves aberrant interactions between a genetically susceptible individual, their microbiota environmental factors. Alterations in xenobiotic receptor expression function are associated with increased risk for IBD. Here, we have assessed role constitutive androstane (CAR), closely related to pregnane X receptor, regulation intestinal mucosal homeostasis....
Proteinase-activated receptors (PARs) are involved in both inflammation and tumorigenesis epithelial cells. Interleukin (IL)-8 is a potent chemoattractant also angiogenesis. The molecular mechanism whereby PARs induce IL-8 expression not known. In HT-29 colonic cells, PAR1 or PAR2 agonists stimulated the of through NF-κB-dependent pathway without inducing IκB degradation disassociation from NF-κB. Further studies revealed that PAR activation induced phosphorylation p65 at Ser-276 nucleus,...
TNF-α activates multiple mitogen-activated protein kinase (MAPK) cascades in intestinal epithelial cells (IECs) leading to the secretion of interleukin 8 (IL-8), a neutrophil chemoattractant and an angiogenic factor with tumor promoting properties. As epidermal growth receptor (EGFR) is known transducer proliferative signals potent activator MAPKs, we hypothesized that EGFR participates TNF-dependent MAPK activation IL-8 by (IECs). We show tyrosine-phosphorylated following treatment IECs...
Proteinase-activated receptor (PAR)(2), a G protein-coupled activated by serine proteinases, has been implicated in both intestinal inflammation and epithelial proliferation. Cyclooxygenase (COX)-2 is overexpressed the gut during as well colon cancer. We hypothesized that PAR(2) drives COX-2 expression cells. Treatment of Caco-2 cancer cells with PAR(2)-activating peptide 2-furoyl-LIGRLO-NH(2) (2fLI), but not its reverse-sequence PAR(2)-inactive peptide, for 3 h led to an increase...
Epithelial surface hydration is critical for proper gut function. However, colonic tissues from individuals with inflammatory bowel disease or animals colitis are hyporesponsive to Cl(-) secretagogues. The secretory responses the muscarinic receptor agonist bethanechol virtually absent in colons of mice dextran sodium sulphate (DSS)-induced colitis. Our aim was define mechanism underlying this cholinergic hyporesponsiveness.Colitis induced by 4% DSS water, given orally. ion transport...
Acetylcholine (ACh) is an important regulator of intestinal epithelial ion transport via muscarinic or nicotinic ACh receptors. Previous studies emphasize the role M 3 receptor subtype in mediating effects cholinergic agonists on transport. With prevalence mouse models to study (patho)physiology, it crucial that be understood this species. Using receptor-deficient (KO) mice and wild-type (WT) mice, we examined contributions as well its colitis induced by dextran sodium sulphate (DSS). In...
Proteinase-activated receptor (PAR)<sub>2</sub> is activated by trypsin-like serine proteinases and has been implicated in intestinal inflammation. However, its role the regulation of mucosal function remains unclear. Using epithelial cell line, SCBN, we have studied stimulus-secretion coupling mechanisms PAR<sub>2</sub>-induced chloride transport, focusing on cyclooxygenase (COX)-1 COX-2 activities prostaglandin (PG) E<sub>2</sub> secretion. SCBN monolayers were grown Snapwell supports,...
Inflammatory bowel diseases are associated with dysregulated electrolyte and water transport resultant diarrhea. Aquaporins transmembrane proteins that function as channels in intestinal epithelial cells. We investigated the effect of inflammatory cytokine, interferon-γ, which is a major player diseases, on aquaporin-1 expression mouse colonic cell line, CMT93. CMT93 monolayers were exposed to 10 ng/mL interferon-γ mRNA protein expressions measured by real-time PCR western blot,...
Abstract Background Clostridium difficile (Cdf) releases toxins (TcdA and TcdB) that damage the intestinal epithelial barrier. Ecto‐5′‐nucleotidase ( CD 73) is expressed on cells, it hypothesized to protect against toxin‐induced through cleavage of 5′‐ AMP adenosine (Ado) subsequent activation receptors (AdoRs). Herein, we sought assess potential protective effects 73 AdoR signaling injurious Cdf toxins. Methods Barrier function was assessed with T84 colonocytes. Transepithelial electrical...
Isoprostanes comprise a class of membrane lipid metabolites produced during oxidative stress, including asthma, chronic obstructive pulmonary disease, and cystic fibrosis. They are widely recognized to evoke variety biological responses in airway vascular smooth muscle, lymphatics, innervation. However, their effects on epithelium largely unstudied. We examined the electrophysiological evoked by several different isoprostane species bovine using Ussing chamber technique. The E-ring...
Chronic inflammatory conditions of the gut including Helicobacter pylori gastritis and bowel diseases ulcerative colitis Crohn's disease are characterized by compromised epithelial barrier function tight junction alterations. Both associated with increased mucosal levels proinflammatory cytokine interleukin‐18 (IL‐18). The role IL‐18 in defects has yet to be investigated. AIM This study assessed effect on gastric intestinal structure function. RESULTS Human (HGE‐20) colonic (Caco‐2)...