A5065450083- Inflammatory Bowel Disease
- Microscopic Colitis
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- Reproductive System and Pregnancy
- IL-33, ST2, and ILC Pathways
- Eosinophilic Esophagitis
- Psoriasis: Treatment and Pathogenesis
- Pregnancy and Medication Impact
- Whipple's Disease and Interleukins
- Acute Lymphoblastic Leukemia research
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Genetic factors in colorectal cancer
- Cytomegalovirus and herpesvirus research
- Helicobacter pylori-related gastroenterology studies
- Immune Response and Inflammation
- Autoimmune and Inflammatory Disorders
- Vitamin D Research Studies
- Diabetes and associated disorders
- Immune responses and vaccinations
- Hepatitis Viruses Studies and Epidemiology
- Biosimilars and Bioanalytical Methods
University of Calgary
2008-2025
Saiseikai Suita Hospital
2022
Kitasato Institute Hospital
2017-2021
Kitasato University
2020-2021
Foothills Medical Centre
2015
Peking Union Medical College Hospital
2014
Chinese Academy of Medical Sciences & Peking Union Medical College
2014
Open Geospatial Consortium
2013
The University of Tokyo
1996-2000
Tokyo University of Agriculture
1997
IL-17 and Foxp3 double-expressing (DE) CD4(+) T lymphocytes are novel crossover immune cell population, but the presence role of these cells in human intestinal inflammation is unclear. The aim this study was to investigate circulating DE patients with inflammatory bowel disease (IBD).The entire cohort consisted 79 subjects: 31 Crohn's disease, 28 ulcerative colitis, 20 healthy control subjects (HC). IBD evidence active at endoscopy were entered into study. Peripheral blood mononuclear used...
In patients with inflammatory bowel disease (IBD) on infliximab, data are limited immune response to influenza vaccine and the impact of timing. The study aims were evaluate responses in IBD infliximab timing responses. this randomized study, 137 subjects maintenance therapy allocated receive 2012/2013 inactivated at time infusion (n = 69) or midway between infusions 68). Serum was collected before after vaccination for hemagglutination inhibition titers. Serologic protection defined by...
Distinction between 2 forms of inflammatory bowel disease (IBD), ulcerative colitis (UC) and Crohn's (CD), can be challenging. Aberrant mucosal immunity suggests that CD is a T helper type 1 cell (Th1)-driven disease, whereas UC as Th2-driven response. However, whether this paradigm truly distinguishes from controversial. We aimed to clarify the discriminating potential lamina propria Th subsets in patients with IBD. Biopsies 79 IBD 20 healthy controls were collected for analysis...
Background . Inflammatory bowel diseases (IBD) occurred in genetically predisposed people exposed to environmental triggers. Diet has long been suspected contribute the development of IBD. Supplementation with n-3 polyunsaturated fatty acids (PUFA) protects against intestinal inflammation rodent models while clinical trials showed no benefits. We hypothesized that intervention timing is crucial and dietary acid pattern may influence environment modify genesis. The aim this study was evaluate...
Pregnancy induces a state of immunological tolerance that aims at suppressing immune responses against the fetus and has been linked to temporal remission preexisting autoimmune disorders. To understand mechanisms this reversible regulation, we investigated role key pregnancy hormone, human chorionic gonadotropin (hCG), in type 1 diabetes nonobese diabetic (NOD) mice.We injected hCG into cytokine gene-deficient NOD mice evaluated effects administration on T-cells dendritic cells (DCs).We...
Distinct CD8+ T-cell subsets such as interleukin-17-expressing Tc17 and Foxp3-expressing Tcreg are functionally similar to CD4+ T cells. Though cells dysregulated in patients with inflammatory bowel disease (IBD), not well investigated. Vitamin D is an environmental factor which influences subsets. We assessed the prevalence of among peripheral blood mononuclear (PBMC) lamina propria (LPMC) Crohn's disease, ulcerative colitis, healthy controls. then tested effect 1α,25-dihydroxyvitamin D3 on...
Inflammatory bowel disease [IBD] results particularly from an aberrance of CD4+ helper and regulatory T cells comprises histopathologically chronic active enterocolitis with features reflecting both activity chronicity mucosal inflammation. The exact immunological–histological correlation in IBD is not understood. We studied the between colonic cell subsets [Th1, Th2, Th17, Th22 Treg] histological changes ulcerative colitis [UC] Crohn’s [CD]. subtyping enumeration were achieved by flow...
The mechanisms underlying the differential effects of cigarette smoking in patients with Crohn's disease (CD) and ulcerative colitis (UC) remain unknown. Smoking has been demonstrated to be protective UC, whereas CD it shown associated a more severe course, frequent relapses, postoperative recurrence. Dendritic cells (DC) play critical role T-cell activation differentiation. Thus, we examined vitro exposure smoke extract (CSE) on phenotype/function DC obtained from UC CD. Sixty-eight...
Abstract Background Hepatitis A virus (HAV) incident infection in Canada is rarely diagnosed (i.e. incidence of 3.6-10 cases/100,000 persons) (PMID: 18159360). Infections generally relate to imported contaminated food-products, or travelers returning from endemic countries. Due its fecal-oral spread, possible underdiagnosis, and often-cryptic presentation, HAV an ideal candidate for wastewater (WW)-based surveillance, a tool increasingly utilized monitor infectious diseases globally.Figure...
Abstract CD1d-restricted Vα14 NKT cells play an important role in both Th1- and Th2-type immune responses. To determine whether develop two functionally distinct subsets that provoke different types of responses, we examined the phenotypes cellular functions NK1.1+ DX5+ T cells. We found are with identical Ag specificities, phenotypes, tissue locations, functions. Similar to NK1.1 marker, DX5 marker (CD49b) is expressed on mature allele-positive allele-negative strains. However, when...
Abstract Stimulated by an agonistic ligand, α-galactosylceramide (αGalCer), invariant NKT (iNKT) cells are capable of both eliciting antitumor responses and suppressing autoimmunity, while they become anergic after initial phase activation. It is unknown how iNKT act as either activators or regulators in different settings cellular immunity. We examined effects αGalCer administration on autoimmune inflammation characterized phenotypes functional status dendritic αGalCer-treated NOD mice....
Abstract Background Fibrocytes are hematopoietic cells with features of mesenchymal found in the circulation and inflammatory sites implicated promoting fibrosis many fibroinflammatory diseases. However, their role(s) development intestinal is poorly understood. Here, we investigated a potential role fibrocytes Crohn’s disease (CD) sought factors that may impact function. Methods Plasma mononuclear were collected from patients without fibrostenotic CD. defined as CD11b+, CD34+, Collagen 1+...
Abstract A burst release of cytokines by Vα14 invariant NKT (iNKT) cells upon their TCR engagement critically regulates innate and adaptive immune responses. However, it remains unclear in vivo why iNKT respond efficiently to microbial or intracellular lipid Ags that are at low levels possess suboptimal antigenicity. We found dendritic (DCs) potentiated a minimal amount ligand α-galactosylceramide (αGalCer) through CD1d-dependent autoreactive responses require endosomal processing CD1d...
Abstract Semi-invariant NK T cell (iNKT) deficiency has long been associated with the pathogenesis of type 1 diabetes (T1D), but linkage between this and T1D susceptibility gene(s) remains unclear. We analyzed NOD mice subcongenic for resistant alleles Idd9 locus in search protective mechanisms against T1D, found that iNKT development was significantly enhanced a more advanced mature phenotype function containing Idd9.1 sublocus B10 origin. The suppressed effector diabetogenic cells....
The relation between the distribution of viral RNA and development histopathological changes was investigated in early stage encephalomyocarditis (EMC) virus-induced orchitis mice. Signals were first detected by situ hybridization a few Sertoli cells almost intact germinal epithelia at 2 days post-inoculation (d.p.i.). number bearing signals increased 3 d.p.i. when mild degenerative exceptionally found epithelia. came to be not only but also small spermatogonia 4 moderate developed...
The pathogenesis of Crohn's disease (CD) involves defects in the innate immune system, impairing responses to microbes. Studies have revealed that mutations NLRP3 are associated with CD. We reported previously Nlrp3-/- mice were more susceptible colitis and exhibited reduced colonic IL-10 expression. In current study, we sought determine how loss might be altering function regulatory T cells, a major source IL-10. Colitis was induced wild-type (WT) by treatment dextran sulphate sodium (DSS)....
Electron microscopic in situ hybridization (ISH-EM) was first applied to the detection of viral RNA germinal epithelium mice inoculated i.p. with 10(5) plaque-forming units/mouse D variant encephalomyocarditis virus (EMC-D). Signals were detected a small number Sertoli cells showing mild degeneration at 2 days post inoculation, and later, they also spermatogonia when showed prominent degeneration. The results clearly demonstrated that site attack cell case EMC-D-induced mouse orchitis.