A5059129490- Hepatitis B Virus Studies
- Neonatal Respiratory Health Research
- Tuberculosis Research and Epidemiology
- Immunotherapy and Immune Responses
- Mycobacterium research and diagnosis
- Fungal Infections and Studies
- Antimicrobial Peptides and Activities
- Escherichia coli research studies
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Congenital Diaphragmatic Hernia Studies
- Gastrointestinal motility and disorders
- Fibroblast Growth Factor Research
- Gut microbiota and health
- vaccines and immunoinformatics approaches
- T-cell and B-cell Immunology
- Neuroscience of respiration and sleep
- Sphingolipid Metabolism and Signaling
- Exercise and Physiological Responses
- Musculoskeletal pain and rehabilitation
- Pharmacology and Obesity Treatment
- Occupational and environmental lung diseases
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Silk-based biomaterials and applications
- Pain Mechanisms and Treatments
Universidade Federal de Sergipe
2017-2024
University of Calgary
2024
Universidade de São Paulo
2007-2021
National Institute of Research in Tuberculosis
2013-2014
Clinics Hospital of Ribeirão Preto
2013-2014
Communication Disorders Technology (United States)
2008
Universidade de Ribeirão Preto
2008
Ministry of Agriculture, Livestock, and Food Supply
2004
Ministério da Agricultura
2004
University of Toronto
1995-1996
Lung branching morphogenesis depends on mesenchymal-epithelial tissue interactions. Keratinocyte growth factor (KGF) has been implicated to be a regulator of these In the present study, we investigated role KGF in early rat lung organogenesis. Reverse transcriptase-polymerase chain reaction analysis revealed mRNA expression mesenchymal component 13-day embryonic lung, while message for receptor (KGFR) was expressed epithelium, confirming paracrine nature KGF/KGFR axis. Antisense...
Abstract Background The greatest challenges in vaccine development include optimization of DNA vaccines for use humans, creation effective single-dose vaccines, delivery systems that do not involve live viruses, and the identification new adjuvants. Herein, we describe a novel, simple technique efficiently vaccinating mice against tuberculosis (TB). Our consists single-dose, genetic formulation DNA-hsp65 complexed with cationic liposomes administered intranasally. Results We developed novel...
The biological role of platelet-derived growth factor (PDGF)-AA in lung morphogenesis was investigated by incubating embryonic explants with phosphorothioate antisense PDGF-A oligonucleotides, which decreased PDGF-AA but not PDGF-BB protein content. Antisense oligonucleotides inhibited DNA synthesis. This inhibitory effect reversed the addition exogenous PDGF-BB. Morphometric analyses antisense-treated cultures showed a significant reduction size. number terminal buds significantly...
Background CD163, receptor for the haptoglobin–hemoglobin complex, is expressed on monocytes/macrophages and neutrophils. A soluble form of CD163 (sCD163) has been associated with M2 macrophage phenotype, macrophages have shown to down-modulate inflammatory responses. In particular, previous studies that closely most severe clinical presentation leprosy (i.e. lepromatous (LL)), as well tuberculosis. We hypothesized sCD163 correlates severity diseases caused by intracellular pathogens....
ABSTRACT There is increasing evidence to suggest that plateletderived growth factor (PDGF) or PDGF-like molecules play a role in fetal lung morphogenesis. Our previous studies demonstrated the presence of PDGF-AA and PDGF-BB homodimers embryonic rat lung. To explore further for development, we conducted intervention using PDGF-B chain-specific antisense oligodeoxynucleotides simple explant system. Unmodified inhibited, concentration-dependent manner, DNA synthesis A maximal inhibition 50%...
The great challenges for researchers working in the field of vaccinology are optimizing DNA vaccines use humans or large animals and creating effective single-dose using appropriated controlled delivery systems. Plasmid encoding heat-shock protein 65 (hsp65) (DNAhsp65) has been shown to induce protective therapeutic immune responses a murine model tuberculosis (TB). Despite success naked DNAhsp65-based vaccine protect mice against TB, it requires multiple doses high amounts immunization. In...
Although it is well established that fibromyalgia (FM) syndrome characterized by chronic diffuse musculoskeletal hyperalgesia, very little known about the effect of this pathology on muscle tissue plasticity. Therefore, present study aimed to characterize putative alterations in skeletal mass female rats subjected a FM model inducing hyperalgesia (CDH) through double injections acidic saline (pH 4.0) into left gastrocnemius at 5-day intervals. To determine protein turnover, total...
Background: Tuberculosis is a major threat to human health. The high disease burden remains unaffected and the appearance of extremely drug-resistant strains in different parts world argues favor urgent need for new effective vaccine. One promising candidates heat-shock protein 65 when used as genetic vaccine (DNAhsp65). Nonetheless, there are substantial data indicating that BCG, only available anti-TB clinical use, provides other important beneficial effects immunized infants. Methods: We...
Our group previously demonstrated that a DNA plasmid encoding the mycobacterial 65-kDa heat shock protein (DNA-HSP65) displayed prophylactic and therapeutic effect in mice model for tuberculosis. This protection was attributed to induction of strong cellular immunity against HSP65. As specific HSP60 family has been detected arthritis, multiple sclerosis diabetes, vaccination procedure with DNA-HSP65 could induce cross-reactive immune response trigger or worsen these autoimmune diseases.In...
Despite the enormous efforts displayed globally in fight against tuberculosis, disease incidence has modified slightly, which led to a renewed interest immunotherapy. In general, successful immunotherapeutic candidates tuberculosis are agents that can trigger strong, specific pro-inflammatory responses, especially of T-helper (Th) 1 pattern. However, how these effectively kill bacteria without eliciting immunopathology is not well understood. We reasoned that, addition immune response...
Transmission of Mycobacterium bovis from cattle to humans has been reported and can cause tuberculosis (Tb) a problem in certain risk populations. Therefore, knowledge resistance M. towards antibiotics used for therapy human Tb could help avoiding cure delay treatment cost increase when dealing with drug resistant organisms. We therefore evaluated the susceptibility isolates streptomycin, isoniazide, rifampicin, ethambutol, ethionamide, first line Tb. 185 clinical samples signs were...
Abstract Objectives Although TB immunotherapy improves the results of conventional drug treatment, effects combining chemotherapy and have never been systematically evaluated. We used a comprehensive lung transcriptome analysis to directly compare activity combined with that single treatments in mouse model TB. Methods Mycobacterium tuberculosis-infected mice chronic phase disease (day 30) received: (i) isoniazid rifampicin (drugs) daily for 30 days; (ii) DNA (DNA), consisting four 100 μg...
How the gut microbiota and immune system maintain intestinal homeostasis in concert with enteric nervous (ENS) remains incompletely understood. To address this gap, we assessed small transit, neuronal density, neurogenesis, microbiota, cell populations cytokines wildtype T-cell deficient germ-free mice colonized specific pathogen-free (SPF) conventionally raised SPF segmented filamentous bacteria (SFB)-monocolonized mice. increased transit a T cell-dependent manner. density myenteric...
Heat-shock proteins (HSPs) are currently one of the most promising targets for development immunotherapy against tumours and autoimmune disorders. This protein family has capacity to activate or modulate function different immune system cells. They induce activation monocytes, macrophages dendritic cells, contribute cross-priming, an important mechanism presentation exogenous antigen in context MHC class I molecules. These various immunological properties HSP have encouraged their use...
Despite substantial efforts in recent years toward the development of new vaccines and drugs against tuberculosis (TB), success has remained elusive. Immunotherapy TB with mycobacterial Hsp65 as a DNA vaccine (DNA-hsp65) results reduction systemic bacterial loads lung tissue damage, but high homology mammalian protein raises concern that pathological autoimmune responses may also be triggered. We searched for elicited by DNA-hsp65 immunotherapy mice chronically infected evaluating humoral...
Exercise-induced hypoalgesia (EIH) is characterized as the pain reduction after an exercise session and it seems to be related release of plasma β-endorphin. In this sense, core stabilization training (CT) has been suggested for patients with chronic nonspecific low back (CNSLBP), but unclear whether induces EIH. Patients CNSLBP have neuromotor dysfunctions that can affect performance functional tasks, thus, performing (FT) could improve motor control promote EIH, since uses multi-joint...
Abstract The gut microbiota and immune system maintain intestinal homeostasis regulate physiology in concert with the enteric nervous (ENS). However, underlying mechanisms remain incompletely understood. Using wildtype T-cell deficient germ-free mice colonized segmented filamentous bacteria (SFB) or specific pathogen-free (SPF) microbiota, we studied regulation of ENS motility. Colonization markedly increased Th17 cells Treg expressing RORγ + T both ileum colon mice. were necessary for...