A5084918804- Neuroendocrine regulation and behavior
- Renin-Angiotensin System Studies
- Stress Responses and Cortisol
- Neuroscience of respiration and sleep
- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Hormonal Regulation and Hypertension
- Circadian rhythm and melatonin
- Birth, Development, and Health
- Electrolyte and hormonal disorders
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Apelin-related biomedical research
- Dietary Effects on Health
- Sodium Intake and Health
- Biochemical Analysis and Sensing Techniques
- Receptor Mechanisms and Signaling
- Cardiovascular Disease and Adiposity
- Heart Rate Variability and Autonomic Control
- Eicosanoids and Hypertension Pharmacology
- Sleep and Wakefulness Research
- Neonatal Respiratory Health Research
- Diet and metabolism studies
- High Altitude and Hypoxia
- Thyroid Disorders and Treatments
- Muscle metabolism and nutrition
Universidade Federal de São Paulo
2018-2025
Universidade Federal de Sergipe
2024
University of Veterinary Medicine Vienna
2022
Bridge University
2022
Universidade de São Paulo
2011-2020
Universidade Federal Rural do Rio de Janeiro
2009-2020
University of Malaya
2015-2019
Universidade de Ribeirão Preto
2011-2017
Fundação Faculdade de Medicina
2015
Universidade Brasil
2013
The respiratory pattern generator modulates the sympathetic outflow, strength of which is enhanced by challenges produced hypoxia. This coupling due to respiratory-modulated presympathetic neurons in rostral ventrolateral medulla (RVLM), but underlining electrophysiological mechanisms remain unclear. For a better understanding neural substrates responsible for generation this respiratory-sympathetic coupling, we combined immunofluorescence, single cell qRT-pCR, and recordings RVLM situ...
Rasd1 is a member of the Ras family monomeric G proteins that was first identified as dexamethasone inducible gene in pituitary corticotroph cell line AtT20. Using microarrays we previously increased mRNA expression rat supraoptic nucleus (SON) and paraventricular (PVN) hypothalamus response to plasma osmolality provoked by fluid deprivation salt loading. RASD1 has been shown inhibit adenylyl cyclase activity vitro resulting inhibition cAMP-PKA-CREB signaling pathway. Therefore, tested...
Concerns over the replicability and reproducibility of published research have grown in many fields, but empirical data to inform policies are still scarce. Biomedical Brazil expanded rapidly last three decades, with no systematic assessment its findings. With this mind, we set up Brazilian Reproducibility Initiative, a multicenter replication experiments from science using common experimental methods: MTT assay, reverse transcription polymerase chain reaction (RT-PCR) elevated plus maze...
Summary We report the effects of a chemically oversulfated chondroitin sulfate and naturally fucosylated on coagulation system. The former has been recently identified as contaminant heparin preparations latter proposed an alternative anticoagulant. mechanism action these polymers is complex target different components They have serpin-independent anticoagulant activity, which preponderates in plasma. also serpin-dependent activity but differ significantly protease preferential serpin. Their...
Salt loading (SL) and water deprivation (WD) are experimental challenges that often used to study the osmotic circuitry of brain. Central this circuit is supraoptic nucleus (SON) hypothalamus, which responsible for biosynthesis hormones, arginine vasopressin (AVP) oxytocin (OXT), their transport terminals reside in posterior lobe pituitary. On challenge evoked by a change blood volume or osmolality, SON undergoes function-related plasticity creates an environment allows appropriate hormone...
We hypothesized that acute stress would induce endothelial dysfunction. Male Wistar rats were restrained for 2 h within wire mesh. Functional and biochemical analyses conducted 24 after the 2-h period of restraint. Stressed showed decreased exploration on open arms an elevated-plus maze (EPM) increased plasma corticosterone concentration. Acute restraint did not alter systolic blood pressure, whereas it in vitro contractile response to phenylephrine serotonin endothelium-intact rat aortas....
Aging affects the body composition and balance of energy metabolism. Here, we collected in a single work several physiological parameters to show how aging sex differences can influence homeostasis. Body mass index (BMI), Lee index, glucose tolerance, glycemia, lipidogram fasting were measured male female Wistar rats at ages 2, 6, 9, 12, 18 months. We also lipid profile, free fatty acids, glycerol, leptin, adiponectin, insulin, corticosterone (CORT), prolactin (PRL), thyroid stimulated...
The Na-K-2Cl cotransporter 2 (NKCC2) was thought to be kidney specific. Here we show expression in the brain hypothalamo-neurohypophyseal system (HNS), wherein upregulation follows osmotic stress. HNS controls stability through synthesis and release of neuropeptide hormone, arginine vasopressin (AVP). AVP travels bloodstream kidney, where it promotes water conservation. Knockdown NKCC2 elicited profound effects on fluid balance following ingestion a high-salt solution—rats produced...
Estrogen receptors are located in important brain areas that integrate cardiovascular and hydroelectrolytic responses, including the subfornical organ (SFO) supraoptic (SON) paraventricular (PVN) nuclei. The aim of this study was to evaluate influence estradiol on neuroendocrine changes induced by hemorrhagic shock ovariectomized rats. Female Wistar rats (220–280 g) were treated for 7 days with vehicle or cypionate (EC, 10 40 μg/kg, sc). On 8th day, animals subjected hemorrhage (1.5 ml/100 g...
Arginine vasopressin (AVP), a neuropeptide hormone that functions in the regulation of water homeostasis by controlling re-absorption at kidneys, is synthesised supraoptic nucleus and paraventricular hypothalamus. An increase plasma osmolality stimulates secretion AVP to blood circulation induces synthesis these nuclei. Although studies on mechanism transcriptional hypothalamus proposed cAMP glucocorticoids positively negatively regulate Avp expression, respectively, molecular mechanisms...
Abstract Active expiration is essential for increasing pulmonary ventilation during high chemical drive (hypercapnia). The lateral parafacial (pF L ) region, which contains expiratory neurones, drives abdominal muscles active in response to hypercapnia. However, the electrophysiological properties and synaptic mechanisms determining activity of pF as well specific conditions their emergence, are not fully understood. Using whole cell electrophysiology single quantitative RT‐PCR techniques,...
Abstract The hormone ghrelin displays several well-characterized functions, including some with pharmaceutical interest. receptor for ghrelin, the growth secretagogue (GHSR), is expressed in hypothalamic paraventricular nucleus (PVH), a critical hub integration of metabolic, neuroendocrine, autonomic, and behavioral functions. Here, we performed neuroanatomical functional characterization neuronal types mediating actions PVH male mice. We found that fluorescent mainly labels neurons...
Background: Hypertension is a polygenic, complex disease that impacts men and women differently; whilst the incidence of high blood pressure (BP) roughly equal over lifetime, typically are at higher risk developing earlier in life, before 50 years age. There adequate evidence brain critical for BP setpoint. The paraventricular nucleus (PVN) hypothalamus an integrative structure can influence not only neurohumoral responses to changes, but also sympathetic drive. Here we manipulate androgenic...
Abstract Aims To evaluate the molecular mechanisms involved in intermittent fasting 16/8 (16/8 IF), a widespread dietary practice adopted worldwide that consists of 16 h and 8 feeding. Methods Obese mice were fasted daily from 6 am to 10 pm. Food intake, body weight, energy expenditure measured. Molecular investigated using ELISA, western blot, qPCR white brown adipose tissues. Glucose homeostasis was also evaluated. Ucp1 knockout ob/ob utilized. Results The IF regimen improved glucose...
ABSTRACT Autism Spectrum Disorder (ASD) presents profound clinical and etiological heterogeneity, complicating the identification of core pathophysiological mechanisms. Single-cell RNA sequencing (scRNA-seq) offers cellular resolution but integrating findings across diverse studies remains challenging. Here, we constructed a unified single-cell reference framework by scRNA-seq data from 11 distinct genetic environmental ASD animal models, encompassing over 300.000 cells various brain regions...
The present work was carried out to investigate the role of angiotensin II type 1 (AT ) receptors in nocturnal thirst and sodium appetite induced by classical models osmotic depletion challenges ovariectomized rats chronically treated with oil or oestradiol benzoate (EB, 20 μg per animal, s.c . daily). In both conditions, animals were given saline losartan (108 nmol i.c.v .), a selective AT receptor blocker. Oestrogen therapy significantly reduced water intake deprivation, produced frusemide...
Secretion of vasopressin (VP), oxytocin (OT) and atrial natriuretic peptide (ANP) is an essential mechanism for the maintenance hydromineral homeostasis. these hormones modulated by several circulating factors, including oestradiol. However, it remains unclear how oestradiol exerts this modulation. In present study we investigated participation in secretion VP, OT ANP activation vasopressinergic oxytocinergic neurones supraoptic (SON) paraventricular (PVN) nuclei hypothalamus response to...