A5067988005- Mesenchymal stem cell research
- Cancer Cells and Metastasis
- Neurogenesis and neuroplasticity mechanisms
- Immune cells in cancer
- Hematopoietic Stem Cell Transplantation
- Barrier Structure and Function Studies
- Muscle Physiology and Disorders
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Pluripotent Stem Cells Research
- Nanoplatforms for cancer theranostics
- Neuroscience and Neuropharmacology Research
- MicroRNA in disease regulation
- SARS-CoV-2 detection and testing
- Nanoparticle-Based Drug Delivery
- Salivary Gland Disorders and Functions
- RNA Research and Splicing
- Bone and Joint Diseases
- Cancer Research and Treatments
- Cancer-related molecular mechanisms research
- Wound Healing and Treatments
- Cancer, Stress, Anesthesia, and Immune Response
- Mosquito-borne diseases and control
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
University of Wisconsin–Madison
2022-2025
Albert Einstein College of Medicine
2015-2025
University of Wisconsin Carbone Cancer Center
2024-2025
Columbia University Irving Medical Center
2017-2024
Universidade Federal de Minas Gerais
2016-2024
Wake Forest University
2011-2018
Universidade de São Paulo
2018
Hudson Institute
2018
John Wiley & Sons (United States)
2018
Winston-Salem/Forsyth County Schools
2013
Tissue growth and function depend on vascularization, vascular insufficiency or excess exacerbates many human diseases. Identification of the biological processes involved in angiogenesis will dictate strategies to modulate reduced excessive vessel formation. We examine essential role pericytes. Their heterogeneous morphology, distribution, origins, physiology have been described. Using double-transgenic Nestin-GFP/NG2-DsRed mice, we identified two pericyte subsets. found that Nestin-GFP −...
Stem cells ensure tissue regeneration, while overgrowth of adipogenic may compromise organ recovery and impair function. In myopathies muscle atrophy associated with aging, fat accumulation increases dysfunction, after chronic injury, the process fatty degeneration, in which is replaced by white adipocytes, further compromises function environment. Some studies suggest that pericytes contribute to regeneration as well formation. This work reports presence two pericyte subpopulations skeletal...
Abstract Introduction Fibrosis, or scar formation, is a pathological condition characterized by excessive production and accumulation of collagen, loss tissue architecture, organ failure in response to uncontrolled wound healing. Several cellular populations have been implicated, including bone marrow-derived circulating fibrocytes, endothelial cells, resident fibroblasts, epithelial recently, perivascular cells called pericytes. We previously demonstrated pericyte functional heterogeneity...
How HSCs populate the fetal liver Hematopoietic stem cells (HSCs) undergo dramatic expansion in before migrating to their definitive site bone marrow. Khan et al. identify portal vessel–associated Nestin + NG2 pericytes as critical HSC niche components (see Perspective by Cabezas-Wallscheid and Trumpp). The vessel expand according fractal geometries, suggesting that cells—rather than factors expressed niche—drive proliferation. After birth, arterial vessels transform into veins, lose...
Abstract A safe and effective vaccine with long-term protection against SARS-CoV-2 variants of concern (VOCs) is a global health priority. Here, we develop lipid nanoparticles (LNPs) to provide delivery plasmid DNA (pDNA) show VOCs in female small animal models. Using library LNPs encapsulating unique barcoded (b-DNA), screen for b-DNA after intramuscular administration. The top-performing are further tested their capacity pDNA uptake antigen-presenting cells vitro. lead LNP used encapsulate...
In older adults, changes in skeletal muscle composition are associated with increased fibrosis, loss of mass, and decreased force, which can lead to dependency, morbidity, mortality. Understanding the biological mechanisms responsible is essential sustaining improving their quality life. Compared young mice, aged mice take longer recover from injury; tissue fibrosis more extensive, regenerated myofibers smaller. Strong evidence indicates that cells called pericytes, embedded basement...
Neuromodulation of deep brain structures (deep stimulation) is the current surgical procedure for treatment Parkinson's disease (PD). Less studied stimulation cortical motor areas to treat PD symptoms, although also known alleviate disturbances in PD. We were able show that optogenetic activation secondary (M2) cortex improves functions dopamine-depleted male mice. The stimulated M2 harbors glutamatergic pyramidal neurons project subcortical structures, critically involved control, and makes...
Symptoms of autonomic failure are frequently the presentation advanced age and neurodegenerative diseases that impair adaptation to common physiologic stressors. The aim this work was examine interaction between sympathetic motor nervous system, involvement system (SNS) in neuromuscular junction (NMJ) presynaptic function, stability postsynaptic molecular organization, skeletal muscle composition function.
Astrocytes are highly specialized glial cells responsible for trophic and metabolic support of neurons. They associated to ionic homeostasis, the regulation cerebral blood flow metabolism, modulation synaptic activity by capturing recycle neurotransmitters maintenance blood-brain barrier. During injuries infections, astrocytes act in defense through heterogeneous progressive changes their gene expression, morphology, proliferative capacity, function, which is known as reactive astrocytes....
Concerns over the replicability and reproducibility of published research have grown in many fields, but empirical data to inform policies are still scarce. Biomedical Brazil expanded rapidly last three decades, with no systematic assessment its findings. With this mind, we set up Brazilian Reproducibility Initiative, a multicenter replication experiments from science using common experimental methods: MTT assay, reverse transcription polymerase chain reaction (RT-PCR) elevated plus maze...