A5025825293- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Lipid Membrane Structure and Behavior
- Synthesis and Biological Evaluation
- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Parasites and Host Interactions
- Toxin Mechanisms and Immunotoxins
- Arsenic contamination and mitigation
- Protein Interaction Studies and Fluorescence Analysis
- Drug Transport and Resistance Mechanisms
- Advanced biosensing and bioanalysis techniques
- Garlic and Onion Studies
- Metal complexes synthesis and properties
- X-ray Diffraction in Crystallography
- Trace Elements in Health
- Advancements in Transdermal Drug Delivery
- Crystallization and Solubility Studies
- Neuroscience and Neuropharmacology Research
- Renin-Angiotensin System Studies
- Venomous Animal Envenomation and Studies
- Bioactive Compounds and Antitumor Agents
- Crystallography and molecular interactions
- Advanced Drug Delivery Systems
- DNA and Nucleic Acid Chemistry
Universidade Federal de Minas Gerais
2016-2025
Instituto de Ciencias Tecnológicas
2001-2015
Centro Universitário de Belo Horizonte
2015
National Institute of Standards and Technology
2014
In-Q-Tel
2012
Sociedade Brasileira de Biofísica
2010
Instituto di Biofisica
2006-2008
Universidade Federal de Ouro Preto
2000-2001
Centre National de la Recherche Scientifique
1989-1998
Université Paris Cité
1990-1998
Abstract A safe and effective vaccine with long-term protection against SARS-CoV-2 variants of concern (VOCs) is a global health priority. Here, we develop lipid nanoparticles (LNPs) to provide delivery plasmid DNA (pDNA) show VOCs in female small animal models. Using library LNPs encapsulating unique barcoded (b-DNA), screen for b-DNA after intramuscular administration. The top-performing are further tested their capacity pDNA uptake antigen-presenting cells vitro. lead LNP used encapsulate...
Extracellular vesicles (EVs) has been considered an alternative process for intercellular communication. EVs release by filamentous fungi and the role of vesicular secretion during fungus-host cells interaction remain unknown. Here, we identified from pathogenic fungus, Aspergillus fumigatus. Analysis structure demonstrated that A. fumigatus produces round shaped bilayer structures ranging 100 to 200 nm size, containing ergosterol a myriad proteins involved in REDOX, cell wall remodeling...
Introduction: Immunotherapy has revolutionized cancer treatment by harnessing the immune system to enhance antitumor responses while minimizing off-target effects. Among promising cancer-specific therapies, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) attracted significant attention. Methods: Here, we developed an ionizable lipid nanoparticle (LNP) platform deliver TRAIL mRNA (LNP-TRAIL) directly microenvironment (TME) induce cell death. Our LNP-TRAIL was formulated via...
ABSTRACT The standard treatment of human leishmaniases involves the use pentavalent antimony [Sb(V)] compounds, including meglumine antimoniate. mode action these compounds has not been fully elucidated. possibility that Sb(III) is involved suggested; however, biomolecule may induce conversion Sb(V) to yet identified. In present study, we investigated both ability reduced glutathione (GSH) promote reduction into in antimoniate and effects pH temperature on this transformation. GSH did a...
Two novel organoantimony(V) and two organobismuth(V) complexes of the type ML2 were synthesized, with L = acetylsalicylic acid (HL1) or 3-acetoxybenzoic (HL2) M triphenylantimony(V) (M1) triphenylbismuth(V) (M2). Complexes, [M1(L1)2] (1), [M1(L2)2]∙CHCl3 (2), [M2(L1)2], (3) [M2(L2)2] (4), characterized by elemental analysis, IR NMR. Crystal structures dicarboxylate 1 2 determined single crystal X-ray diffraction. Structural analyses revealed that adopt five-coordinated extremely distorted...
Background Drug resistance is a major problem in leishmaniasis chemotherapy. RNA expression profiling using DNA microarrays suitable approach to study simultaneous events leading drug-resistance phenotype. Genomic analysis has been performed primarily with Old World Leishmania species and here we investigate molecular alterations antimony the New L. amazonensis. Methods/Principal Findings We selected populations of amazonensis promastigotes for by step-wise drug pressure. Gene highly...
Mesenchymal stem/stromal cells (MSCs) are promising tools in cell therapy. They secrete extracellular vesicles (EVs) that carry different classes of molecules can promote skin repair, but the mechanisms poorly understood. Skin wound healing is a complex process requires activity several signaling pathways and types, including keratinocytes fibroblasts. In this study, we explored whether adipose tissue MSC-derived EVs could accelerate migration proliferation fibroblasts, activate AKT pathway,...
Antimony resistance complicates the treatment of infections caused by parasite Leishmania.Using next generation sequencing, we sequenced genome four independent Leishmania guyanensis antimony-resistant (SbR) mutants and found different chromosomal alterations including aneuploidy, intrachromosomal gene amplification deletion. A segment covering 30 genes on chromosome 19 was amplified intrachromosomally in three mutants. The coding for multidrug associated protein involved antimony also...
Gene therapy is a promising approach to be applied in cardiac regeneration after myocardial infarction and gene correction for inherited cardiomyopathies. However, cardiomyocytes are crucial cell types that considered hard-to-transfect. The entrapment of nucleic acids non-viral vectors, such as lipid nanoparticles (LNPs), an attractive safe effective delivery.Here, mini-library engineered LNPs was developed pDNA delivery cardiomyocytes. were characterized screened identified lead LNP...
One of the phenotypes multidrug resistance is characterized by a decrease in intracellular concentration drug resistant cells as compared to sensitive cells. This correlated with presence membrane 150–180‐kDa glycoprotein, P‐glycoprotein, responsible for an active efflux drug. The fluorescence emission spectra from anthracycline‐treated suspended buffer have been used compare transport five anthracycline derivatives: adriamycin, daunorubucin, 4′‐ o ‐tetrahydropyranyladriamycin, carminomycin...
The toxicity and antileishmanial effectiveness of a novel liposome formulation meglumine antimoniate in mongrel dogs with visceral leishmaniasis (VL) obtained from region where VL is endemic Brazil have been investigated. Groups 12 animals received by the intravenous route four doses (with 4-day intervals) either liposomal (group I [GI], 6.5 mg Sb/kg body weight/dose), empty liposomes (GII), or isotonic saline (GIII). Evaluation markers hematopoietic, hepatic, renal functions before just...