A5022327802- Immune Response and Inflammation
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- S100 Proteins and Annexins
- Neuropeptides and Animal Physiology
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Neonatal Respiratory Health Research
- Receptor Mechanisms and Signaling
- Vitamin C and Antioxidants Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Natural product bioactivities and synthesis
- Renin-Angiotensin System Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Protease and Inhibitor Mechanisms
- Drug-Induced Adverse Reactions
- Immune responses and vaccinations
- Chromatography in Natural Products
- Gout, Hyperuricemia, Uric Acid
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Biomarkers in Disease Mechanisms
- Complement system in diseases
- Medicinal plant effects and applications
- Sepsis Diagnosis and Treatment
Universidade Federal de Minas Gerais
2019-2025
Universidade de Ribeirão Preto
2025
Universidade de São Paulo
2025
Brigham and Women's Hospital
2024
Macrophages are central to inflammation resolution, an active process aimed at restoring tissue homeostasis following inflammatory response. Here, the effects of db-cAMP on macrophage phenotype and function were investigated. Injection into pleural cavity mice induced monocytes recruitment in a manner dependent PKA CCR2/CCL2 pathways. Furthermore, promoted reprogramming bone-marrow-derived macrophages M2 as seen by increased Arg-1/CD206/Ym-1 expression IL-10 levels (M2 markers). Db-cAMP also...
Sepsis is a lethal syndrome characterized by systemic inflammation and abnormal coagulation. Despite therapeutic advances, sepsis mortality remains substantially high. Herein, we investigated the role of plasminogen/plasmin (Plg/Pla) system during sepsis. Plasma levels Plg were significantly lower in mice subjected to severe compared with nonsevere sepsis, whereas IL-6, marker severity, higher correlated negatively IL-6 both septic patients, plasminogen activator inhibitor-1 positively IL-6....
Biochanin A (BCA) is a natural organic compound of the class phytochemicals known as flavonoids and isoflavone subclass predominantly found in red clover ( Trifolium pratense ). It has anti-inflammatory activity some pro-resolving actions, such neutrophil apoptosis. However, effect BCA resolution inflammation still poorly understood. In this study, we investigated effects on neutrophilic inflammatory response its model antigen-induced arthritis. Male wild-type BALB/c mice were treated with...
Macrophages are professional phagocytes that display remarkable plasticity, with a range of phenotypes can be broadly characterized by the M1/M2 dichotomy. Glucocorticoid (GC)-induced leucine zipper (GILZ) is protein known to mediate anti-inflammatory and some pro-resolving actions, including as neutrophil apoptosis. However, role GILZ in key macrophage function not well understood. Here, we investigated on reprogramming efferocytosis. Using murine bone-marrow-derived macrophages (BMDMs),...
Nonphlogistic migration of macrophages contributes to the clearance pathogens and apoptotic cells, a critical step for resolution inflammation return homeostasis. Angiotensin-(1-7) [Ang-(1-7)] is heptapeptide renin-angiotensin system that acts through Mas receptor (MasR). Ang-(1-7) has recently emerged as novel proresolving mediator, yet mechanisms are not fully determined. Herein, stimulated human murine monocytes/macrophages in MasR-, CCR2-, MEK/ERK1/2-dependent manner. Pleural injection...
ABSTRACT Severe coronavirus outbreaks, including SARS, MERS, and COVID‐19, have underscored the urgent need for effective antiviral therapies. This study evaluated activity of biflavanones isolated from Ouratea spectabilis —specifically ouratein (Our‐) A, B, C, D—against murine hepatitis virus (MHV‐3) human SARS‐CoV‐2. Cells infected with MHV‐3 or SARS‐CoV‐2 were treated ourateins, viral replication was assessed using plaque assays. Mice Our‐D either orally intraperitoneally. Key assessments...
ABSTRACT Non-canonical conjugation of ATG8 proteins, including LC3, to single membranes implicates the autophagy machinery in cell functions unrelated metabolic stress. One such pathway is LC3-associated phagocytosis (LAP), which aids phagosome maturation and subsequent signaling upon cargo uptake mediated by certain innate immunity-associated receptors. Here, we show that a specific isoform RAB5 GTPases, molecular switches controlling early endosome traffic, necessary for LAP. We...
Gram-negative bacteria are the main causes of pneumonia in nosocomial environment. Inflammation triggered during is necessary for bacterial clearance but must be spatially and temporally regulated to prevent further tissue damage dissemination. Emerging data shows that annexin A1 (ANXA1) signalling through its receptor FPR2 promotes host resistance resilience preclinical models infectious disease. Using a mouse model Escherichia coli-induced pneumonia, role ANXA1/FPR2 on key steps...
Pneumonia is a leading cause of morbidity and mortality. While inflammation host protective response that ensures bacterial clearance, finely regulated necessary to prevent bystander tissue damage. Glucocorticoid (GC)-induced leucine zipper (GILZ) GC-induced protein with anti-inflammatory proresolving bioactions, yet the therapeutical role GILZ in infectious diseases remains unexplored. Herein, we investigate effects during acute lung injury (ALI) induced by LPS Streptococcus pneumoniae...
Gouty arthritis (GA) is an inflammatory triggered by the deposition of monosodium urate monohydrate (MSU) crystals, causing pain, inflammation, and joint damage. Several drugs are currently employed to manage acute flares GA, but they either have limited effectiveness or induce severe adverse reactions. Ouratea spectabilis traditionally used in Brazil treat gastric ulcers rheumatism. The ethanolic extract O. stems (OSpC) four biflavanones (ouratein A - D) isolated thereof were evaluated a...
Macrophages are important effectors of inflammation resolution that contribute to the elimination pathogens and apoptotic cells restoration homeostasis. Pre-clinical studies have evidenced anti-inflammatory pro-resolving actions GILZ (glucocorticoid-induced leucine zipper). Here, we evaluated role on migration mononuclear under nonphlogistic conditions Escherichia coli-evoked peritonitis. TAT-GILZ (a cell-permeable GILZ-fusion protein) injection into pleural cavity mice induced...
Abstract Pseudomonas aeruginosa is a frequent cause of antimicrobial‐resistant hospital‐acquired pneumonia, especially in critically ill patients. Inflammation triggered by P. infection necessary for bacterial clearance but must be spatially and temporally regulated to prevent further tissue damage dissemination. Emerging data have shed light on the pro‐resolving actions angiotensin‐(1‐7) [Ang‐(1‐7)] signaling through G protein‐coupled receptor Mas (MasR) during infections. Herein, we...
<title>Abstract</title> <bold>Objective: </bold>Pro-resolving molecules, including the peptide Angiotensin-(1-7) [Ang-(1-7)], have potential adjunctive therapy for infections. Here we evaluate actions of Ang-(1-7) in betacoronavirus infection mice. <bold>Methods: </bold>C57BL/6 mice were infected intranasally with murine MHV-3 and K18-hACE2 SARS-CoV-2. Mice treated (30 μg/mouse, i.p.) at 24-, 36-, 48-hours post-infection (hpi) or 24, 36, 48, 72, 96 h. For lethality evaluation, one additional...