A5061432370- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Reproductive System and Pregnancy
- CAR-T cell therapy research
- Endometrial and Cervical Cancer Treatments
- NF-κB Signaling Pathways
- Immune cells in cancer
- Cell Adhesion Molecules Research
- PI3K/AKT/mTOR signaling in cancer
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Chronic Lymphocytic Leukemia Research
- Cytokine Signaling Pathways and Interactions
- Hepatitis C virus research
- Signaling Pathways in Disease
- Galectins and Cancer Biology
- Glycosylation and Glycoproteins Research
- Acute Myeloid Leukemia Research
- Adipokines, Inflammation, and Metabolic Diseases
- IL-33, ST2, and ILC Pathways
- Diabetes and associated disorders
- Immunodeficiency and Autoimmune Disorders
- Phagocytosis and Immune Regulation
Inserm
2015-2025
Centre National de la Recherche Scientifique
2014-2025
Aix-Marseille Université
2015-2025
Centre de Recherche en Cancérologie de Marseille
2015-2024
Immunité et Cancer
2017-2024
Institut Paoli-Calmettes
2012-2022
Institut Pprime
2021-2022
French Clinical Research Infrastructure Network
2022
Canadian Nautical Research Society
2021
Fondation pour la Recherche Médicale
2018-2020
Natural killer (NK) cells are lymphocytes of the innate immune system that involved in early defenses against foreign cells, as well autologous undergoing various forms stress, such microbial infection or tumor transformation. NK cell activation is controlled by a dynamic balance between complementary and antagonistic pathways initiated upon interaction with potential target cells. express an array activating surface receptors can trigger cytolytic programs, cytokine chemokine secretion....
The programmed death-1 (PD-1) molecule is involved in peripheral tolerance and the immune escape mechanisms during chronic viral infections cancer. PD-1 interacts with two ligands, PD-L1 PD-L2. We have investigated molecular of interactions its ligands by surface plasmon resonance cell binding as well ability to compete for binding. PD-L2 bound comparable affinities, but striking differences were observed at level association dissociation characteristics. PD-L1, not PD-L2, had a delayed...
Abstract IFN-α is an important cytokine for the generation of a protective T cell-mediated immune response to viruses. In this study, we asked whether can regulate functional properties dendritic cells (DCs). We show that monocytes cultured in presence GM-CSF and differentiate into DCs (IFN-α-derived (IFN-DCs)). When compared with generated IL-4 (IL-4-derived DCs), IFN-DCs exhibited typical DC morphology expressed, addition markers CD1a blood Ag 4, similar level costimulatory class II MHC...
The success and limitations of current immunotherapies have pushed research toward the development alternative approaches possibility to manipulate other cytotoxic immune cells such as natural killer (NK) cells. Here, we targeted an intracellular inhibiting protein 'cytokine inducible SH2-containing protein' (CISH) in NK evaluate impact on their functions antitumor properties.To further understand CISH cells, developed a conditional Cish-deficient mouse model (Cishfl/flNcr1Ki/+ ). cytokine...
Abstract Cervical tumors are usually treated using surgery, chemotherapy, and radiotherapy, would benefit from immunotherapies. However, the immune microenvironment in cervical cancer remains poorly described. Tertiary lymphoid structures (TLS) were recently described as markers for better immunotherapy response overall prognosis patients. We evaluated tumor microenvironment, specifically focusing on TLS, combined high-throughput phenotyping, soluble factor concentration dosage TME spatial...
<div>Abstract<p>Cervical tumors are usually treated using surgery, chemotherapy, and radiotherapy would benefit from immunotherapies. However, the immune microenvironment in cervical cancer remains poorly described. Tertiary lymphoid structures (TLS) were recently described as markers for better immunotherapy response overall prognosis patients with cancer. We evaluated tumor microenvironment, specifically focusing on TLS, combined high-throughput phenotyping, soluble factor...
CD28 is a 44-kD homodimer expressed on the surface of majority human T cells that provides an important costimulus for cell activation. The biochemical basis accessory signals poorly understood. Triggering antigen receptor (TCR) activates p21ras proteins. Here we show ligation by monoclonal antibody (mAb) also stimulates and induces Ras-dependent events such as stimulation microtubule-associated protein (MAP) kinase ERK2 hyperphosphorylation Raf-1. One physiological ligand molecule B7-1. In...
Abstract ICOS ligation in concert with TCR stimulation results strong PI3K activation T lymphocytes. The cytoplasmic tail contains an YMFM motif that binds the p85α subunit of class IA PI3K, similar to YMNM CD28, suggesting a redundant function two receptors signaling. However, costimulation shows greater activity than CD28 cells. We show this report expression activated cells triggers participation p50α, one regulatory subunits PI3Ks. Using different T-APC cell conjugate systems, we p50α...
Women with low levels of vitamin D have a higher risk developing breast cancer. Numerous studies associated the presence CD8+ T cell infiltration good prognosis. As may play key role in modulation immune system, objective this work was to evaluate impact on cancer progression and mammary tumor microenvironment. We show that decreases growth. Immunomonitoring different subsets dissociated tumors revealed an increase infiltrating cells D-treated group. Interestingly, these exhibited more...
Natural killer (NK) cells are major antileukemic immune effectors. Leukemic blasts have a negative impact on NK cell function and promote the emergence of phenotypically functionally impaired cells. In current work, we highlight an accumulation CD56-CD16+ unconventional in acute myeloid leukemia (AML), aberrant subset initially described as being elevated patients chronically infected with HIV-1. Deep phenotyping was performed using peripheral blood from newly diagnosed AML (n = 48,...
The phosphatase and tensin homologue deleted on chromosome 10 (PTEN) negatively regulates cell survival proliferation mediated by phosphoinositol 3 kinases. We have explored the role of phosphoinositol(3,4,5)P3-phosphatase PTEN in T development analyzing mice with a cell–specific deletion PTEN. Ptenflox/floxLck-Cre developed thymic lymphomas, but before onset tumors, they showed normal cellularity. To reveal regulatory developing cells we crossed PTEN-deficient deficient for interleukin...
Abstract The human butyrophilin (BTN) 3 or CD277 molecules belong to the B7 family members and are expressed in various immune cells such as T NK cells. Here, we show that triggering considerably enhances TCR‐induced cytokine production cell proliferation, even when another co‐stimulatory molecule, CD28, is engaged. These CD277‐induced additive functional effects accordance with detection of early T‐cell activation events signaling being increased upon engagement. However, found not involved...
Accumulating evidence highlights natural killer (NK) cell parameters as potential prognostic factors in cancer patients, which provides a strong rationale for developing therapeutic strategies aiming at restoring NK cell. However, reaching this point warrants better characterization of tumor-induced alterations. Our group recently reported heterogeneous maturation acute myeloid leukemia (AML) patients. the clinical significance such observations remained to be assessed on larger cohort based...
Tumor necrosis factor superfamily member 14 (TNFRSF14)/herpes virus entry mediator (HVEM) is the ligand for B and T lymphocyte attenuator (BTLA) CD160-negative immune co-signaling molecules as well viral proteins. Its expression dysregulated with an overexpression in tumors a connection of adverse prognosis.We developed C57BL/6 mouse models co-expressing human (hu)BTLA huHVEM antagonistic monoclonal antibodies (mAbs) that completely prevent interactions HVEM its ligands.Here, we show...
mRNA applications have undergone unprecedented applications-from vaccination to cell therapy. Natural killer (NK) cells are recognized a significant potential in immunotherapy. NK-based therapy has drawn attention as allogenic graft with minimal graft-versus-host risk leading easier off-the-shelf production. NK can be engineered either viral vectors or electroporation, involving high costs, risks, and toxicity, emphasizing the need for alternative way technology. We successfully developed,...
This study compares the biochemical responses in T cells activated with CD28 ligands B7-1 and B7-2. The patterns of tyrosine phosphorylation induced by these two are identical, but clearly different from cell receptor (TCR). TCR regulates protein complexes mediated adapter Grb2 both vivo vitro. In contrast, there is no apparent regulation response to or Rather, B7-2 induce a protein, p62. p62 unique that not shared TCR. These data indicate identical kinase signal transduction pathways. show...
Journal Article CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events Get access Jacques Nunès, Nunès Search for other works by this author on: Oxford Academic PubMed Google Scholar Sandrine Klasen, Klasen Marguerite Ragueneau, Ragueneau Christine Pavon, Pavon Dominique Couez, Couez Claude Mawas, Mawas Marcello Bagnasco, Bagnasco 1DI.M.I., Universita' di GenovaViale Benedetto XV, n.6 16132 Genova, Italy...