A5030206344- Immune Cell Function and Interaction
- European Political History Analysis
- T-cell and B-cell Immunology
- Historical Studies and Socio-cultural Analysis
- Genomics and Chromatin Dynamics
- NF-κB Signaling Pathways
- Medieval and Early Modern Justice
- French Urban and Social Studies
- Immune Response and Inflammation
- Health, Medicine and Society
- HIV Research and Treatment
- CAR-T cell therapy research
- Virus-based gene therapy research
- Cytokine Signaling Pathways and Interactions
- RNA Research and Splicing
- Social Policies and Family
- Classical Studies and Legal History
- Medieval European Literature and History
- Medieval Literature and History
- Healthcare Systems and Practices
- Cancer Research and Treatments
- Polyomavirus and related diseases
- Trace Elements in Health
- Historical Legal Studies and Society
- Multiculturalism, Politics, Migration, Gender
Inserm
2013-2025
Centre National de la Recherche Scientifique
1997-2025
Montpellier GenomiX
2025
Université de Montpellier
2025
Aix-Marseille Université
2013-2020
Theories and Approaches of Genomic Complexity
2010-2019
Institut de Neurobiologie de la Méditerranée
2008-2019
Centre de Recherche en Cancérologie de Marseille
2004-2019
Centre d’Immunologie de Marseille-Luminy
2009-2012
Vitenparken
2012
Natural killer (NK) cells contribute to a variety of innate immune responses viruses, tumors and allogeneic cells. However, our understanding NK cell biology is severely limited by the lack consensus phenotypic definition these across species, specific marker visualize them in situ , genetic model where may be selectively ablated. NKp46/CD335 an Ig-like superfamily surface receptor involved human activation. In addition human, we show here that NKp46 expressed all mouse strains analyzed, as...
The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental the life cycle, yet underlying molecular details remain obscure. Here we show that an essential step genome-wide incorporation TH2B, histone H2B variant hitherto unknown function. Using mouse models in which TH2B depleted or C-terminally modified, directs final transformation dissociating nucleosomes into protamine-packed structures. Depletion induces compensatory mechanisms permit removal by...
Divergent transcription is a wide-spread phenomenon in mammals. For instance, short bidirectional transcripts are hallmark of active promoters, while longer can be detected antisense from genes conditions where the RNA degradation machinery inhibited. Moreover, many described long non-coding RNAs (lncRNAs) transcribed coding gene promoters. However, general significance divergent lncRNA/mRNA pair still poorly understood. Here, we used strand-specific RNA-seq with high sequencing depth to...
Vanin-1 is an epithelial ectoenzyme with pantetheinase activity and generating the amino-thiol cysteamine through metabolism of pantothenic acid (vitamin B(5)). Here we show that Vanin-1(-/-) mice, which lack in tissues, exhibit resistance to oxidative injury induced by whole-body gamma-irradiation or paraquat. This protection correlated reduced apoptosis inflammation reversed treating mutant animals cystamine. The better tolerance mice associated enhanced gamma-glutamylcysteine synthetase...
RNA-seq was used to study the response of Desulfovibrio hydrothermalis, isolated from a deep-sea hydrothermal chimney on East-Pacific Rise at depth 2,600 m, various hydrostatic pressure growth conditions. The transcriptomic datasets obtained after 26, 10 and 0.1 MPa identified only 65 differentially expressed genes that were distributed among four main categories: aromatic amino acid glutamate metabolisms, energy metabolism, signal transduction, unknown function. gene expression patterns...
To better understand why human neonates show a poor response to intracellular pathogens, we compared gene expression and histone modification profiles of neonatal naive CD8+ T cells with that their adult counterparts. We found lymphocytes have distinct epigenomic landscape associated lower genes involved in cell receptor (TCR) signaling cytotoxicity higher the cycle innate immunity. Functional studies corroborated are less cytotoxic, transcribe antimicrobial peptides, produce reactive oxygen...
The interleukin 2 receptor ␣-chain (IL-2R␣) gene is a key regulator of lymphocyte proliferation.IL-2R␣ rapidly and potently induced in T cells response to mitogenic stimuli.Interleukin (IL-2) stimulates IL-2R␣ transcription, thereby amplifying expression its own high-affinity receptor.IL-2R␣ transcription at least part controlled by two positive regulatory regions, PRRI PRRII.PRRI an inducible proximal enhancer, located between nucleotides ؊276 ؊244, which contains NF-B SRE/CArG motifs.PRRII...
Ku protein is a relatively abundant DNA-binding nuclear complex composed of two polypeptide subunits, p70 and p80. has been recently identified as the regulatory component DNA-dependent kinase that phosphorylates RNA polymerase II. To further characterize in vivo regulation protein, we studied expression transcripts coding for p80 subunits different human cell lines normal tissues by Northern blot hybridization, using specific cDNA probes. The level both genes was approximately 10-fold...
Transcription implies recruitment of RNA polymerase II and transcription factors (TFs) by DNA melting near start site (TSS). Combining atomic force microscopy computer modeling, we investigate the structural dynamical properties IL2RA promoter identify an intrinsically negative supercoil in PRRII region (containing Elf-1 HMGA1 binding sites), located upstream a curved encompassing TSS. Conformational changes, evidenced time-lapse studies, result progressive positioning curvature apex towards...
Ets1 is a sequence-specific transcription factor that plays an important role during hematopoiesis, and essential for the transition of CD4−/CD8− double negative (DN) to CD4+/CD8+ positive (DP) thymocytes. Using genome-wide functional approaches, we investigated binding properties, transcriptional chromatin environment this transition. We found while at distal sites was associated with active genes both DN DP stages, its enhancer activity attained stage, as reflected by levels core hallmarks...
Glioblastomas are the most common primary brain tumors, highly vascularized, infiltrating, and resistant to current therapies. This cancer leads a fatal outcome in less than 18 months. The aggressive behavior of glioblastomas, including resistance treatments tumor recurrence, has been attributed glioma stemlike/progenitor cells. transcription factor EGR1 (early growth response 1), member zinc finger family, described as suppressor gliomas when ectopically overexpressed. Although expression...
Abstract HIV-1 infection is associated with a progressive and functional decline in the CD4+ lymphoid Th1 subset. Here, we propose that HIV nef gene product may function as specific regulator of cytokine production. By use T cell-specific inducible expression system, show upon cell activation, induced down-regulated both IL-2 IFN-gamma production dose-dependent manner, whereas IL-4, IL-9, IL-13, IL-8, TNF-alpha remained unaffected. In addition to this, independent transfected clones...
ABSTRACT Insulin-mediated cell motility as well the role of transcription factors in insulin-activated intracellular signal events have not been extensively studied. In this report we examined whether insulin could mediate haptotactic migration cultured human epidermal keratinocytes through activation factor NF-κB. Insulin caused a dose-dependent stimulation keratinocyte that maximally reached 2-fold at 2×10−7 M hormone. This phenomenon was independent nature extracellular matrix component...
Metallothionein (MT) gene promoters in higher eucaryotes contain multiple metal regulatory elements (MREs) that are responsible for the induction of MT transcription. We identified and purified to near homogeneity a 74-kilodalton mouse nuclear protein specifically binds certain MRE sequences. This protein, MBF-I, was employing as an affinity reagent trout is shown be functional cells but which lacks G+C-rich SP1-like sequences found many mammalian promoters. Using point-mutated MREs, we...
SEMA3F is a secreted semaphorin with potent antitumor activity, which frequently downregulated in lung cancer. In cancer cell lines, overexpression decreases hypoxia-induced factor 1α protein and vascular endothelial growth mRNA, inhibits multiple signaling components. Therefore, understanding how expression inhibited cells important. We previously defined the promoter organization of found that chromatin remodeling by histone deacetylase inhibitor was sufficient to activate expression....
We have followed Sp1 expression in primary human T lymphocytes induced, via CD2 plus CD28 costimulation, to sustained proliferation and subsequent return quiescence. Binding of wheat germ agglutinin lectin was not modified following activation, indicating that the overall glycosylation protein unchanged. underwent, instead, a major dephosphorylation correlated with cyclin A and, thus, cell cycle progression. similar change observed cells re-entered secondary interleukin-2 stimulation, as...