A5025097671- Photosynthetic Processes and Mechanisms
- Microbial Community Ecology and Physiology
- Microbial Fuel Cells and Bioremediation
- Metalloenzymes and iron-sulfur proteins
- Metal-Catalyzed Oxygenation Mechanisms
- Hemoglobin structure and function
- Genomics and Phylogenetic Studies
- Mine drainage and remediation techniques
- Mass Spectrometry Techniques and Applications
- Methane Hydrates and Related Phenomena
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Electrochemical Analysis and Applications
- Wastewater Treatment and Nitrogen Removal
- Protist diversity and phylogeny
- Porphyrin and Phthalocyanine Chemistry
- Electrocatalysts for Energy Conversion
- Spectroscopy and Quantum Chemical Studies
- Bacterial Genetics and Biotechnology
- ATP Synthase and ATPases Research
- Redox biology and oxidative stress
- Metal Extraction and Bioleaching
- Porphyrin Metabolism and Disorders
- Microbial bioremediation and biosurfactants
- Microbial Metabolic Engineering and Bioproduction
Centre National de la Recherche Scientifique
2013-2024
Aix-Marseille Université
1989-2024
Université de Toulon
2018-2024
Institut de Recherche pour le Développement
2018-2024
Institut Méditerranéen d’Océanologie
2017-2024
Laboratoire de Chimie Bactérienne
1987-2015
Institut de Microbiologie de la Méditerranée
2011-2015
Physique des interactions ioniques et moléculaires
2010-2011
Délégation Provence et Corse
1990-2010
Universidade Nova de Lisboa
2000-2009
Due to its capacity produce large amounts of cellulases, Trichoderma reesei is increasingly been researched in various fields white biotechnology, especially biofuel production from lignocellulosic biomass. The commercial enzyme mixtures produced at industrial scales are not well characterized, and their proteinaceous components poorly identified quantified. development proteomic methods has made it possible comprehensively overview the enzymes involved biomass degradation which secreted...
RNA-seq was used to study the response of Desulfovibrio hydrothermalis, isolated from a deep-sea hydrothermal chimney on East-Pacific Rise at depth 2,600 m, various hydrostatic pressure growth conditions. The transcriptomic datasets obtained after 26, 10 and 0.1 MPa identified only 65 differentially expressed genes that were distributed among four main categories: aromatic amino acid glutamate metabolisms, energy metabolism, signal transduction, unknown function. gene expression patterns...
ABSTRACT Two mutant strains of Desulfovibrio vulgaris Hildenborough lacking either the sod gene for periplasmic superoxide dismutase or rbr rubrerythrin, a cytoplasmic hydrogen peroxide (H 2 O ) reductase, were constructed. Their resistance to oxidative stress was compared that wild-type and sor reductase. The more sensitive exposure air internally externally generated than mutant, which in turn strain. No obvious phenotype found indicating H may also be conferred by two other genes D....
Desulfovibrio piezophilus strain C1TLV30T is a piezophilic anaerobe that was isolated from wood falls in the Mediterranean deep-sea. D. represents unique model for studying adaptation of sulfate-reducing bacteria to hydrostatic pressure. Here, we report 3.6 Mbp genome sequence this bacterium. An analysis revealed presence seven genomic islands as well gene clusters are most likely linked life at high Comparative genomics and differential proteomics identified transport solutes amino acids...
The ability to respire sulfate linked lactate oxidation is a key metabolic signature of the Desulfovibrio genus. Lactate by these incomplete oxidizers generates reductants through dehydrogenase (LDH) and pyruvate-ferredoxin oxidoreductase (PFOR), with latter catalyzing pyruvate conversion into acetyl-CoA. Acetyl-CoA source substrate-level phosphorylation though production ATP. Here, we show that crucial steps are performed enzymes encoded nonacistronic transcriptional unit named now as...
ABSTRACT The physiological properties of a hyd mutant Desulfovibrio vulgaris Hildenborough, lacking periplasmic Fe-only hydrogenase, have been compared with those the wild-type strain. hydrogenase is main D. which also has NiFe- and NiFeSe-hydrogenases. grew less well than strain in media sulfate as electron acceptor H 2 sole donor, especially at high concentration. Although mutation had little effect on growth lactate donor for reduction when was present, lactate- sulfate-containing...
Archaeoglobus fulgidus oxidizes fatty acids (C(4) to C(18)) and n-alk-1-enes (C(12:1) C(21:1)) in the presence of thiosulfate as a terminal electron acceptor. End products metabolism were CO(2) sulfide. Growth on perdeuterated hexadecene yielded C(15)- C(17)-labeled metabolites, thus confirming ability A. oxidize alkyl chains.
Although obligate anaerobe, the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough (DvH) exhibits high aerotolerance that involves several enzymatic systems, including two membrane-bound oxygen reductases, a bd-quinol oxidase and cc(b/o)o3 cytochrome oxidase. Effect of constant low concentration on growth morphology wild-type, single (Δbd, Δcox) double deletion (Δcoxbd) mutant strains genes encoding these reductases was studied. When both wild-type were cultured in...
Sulfate-reducing bacteria, like Desulfovibrio vulgaris Hildenborough, have developed a set of reactions allowing them to survive in oxic environments and even reduce molecular oxygen water. D. contains cytoplasmic superoxide reductase (SOR) periplasmic dismutase (SOD) involved the elimination anions. To assign function SOD, [Fe] hydrogenase activity was followed both wild-type sod deletant strains. This lower strain lacking SOD than when cells were exposed for short time. The is thus...
Hydrogen uptake and evolution reactions have been electrochemically measured using a glassy carbon electrode modified by coating made of whole Desulfovibrio vulgaris Hildenborough cells. High cathodic anodic catalytic currents obtained methyl viologen as mediator, which are reflecting hydrogenase activity inside the bacterial cell, in production hydrogen, respectively. The influence various parameters, such pH, ionic strength nature mediator on current values has studied. An original...
Cytoplasmic membranes of the strictly anaerobic sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough contain two terminal oxygen reductases, a bd quinol oxidase and cc(b/o)o3 cytochrome (Cox). Viability assays pointed out that single Δbd, Δcox double ΔbdΔcox deletion mutant strains were more sensitive to exposure than WT strain, showing involvement these reductases in detoxification oxygen. The strain was slightly Δbd pointing importance protection. Decreased O2 reduction rates...
Formate dehydrogenases (FDHs) are enzymes that catalyze the formate oxidation to carbon dioxide and contain either Mo or W in a mononuclear form active site. In present work, influence of salts on production FDH by Desulfovibrio alaskensis NCIMB 13491 was studied. Two different FDHs, one containing (W-FDH) second incorporating (Mo/W-FDH), were purified. Both isolated from cells grown medium supplemented with 1 μM molybdate, whereas only W-FDH purified cultured 10 tungstate. We demonstrated...
Recent years have seen significant progress in understanding basic bacterial cell cycle properties such as growth and division. While characterization regulation of is quite well-documented the case fast growing aerobic model organisms, no data has been so far reported for anaerobic bacteria. This lack information microorganisms can mainly be explained by absence molecular cellular tools single microscopy fluorescent probes usable anaerobes essential to study events and/or subcellular...
Mesotoga prima strain PhosAc3 is a mesophilic representative of the phylum Thermotogae comprising only fermentative bacteria so far. We show that while unable to ferment glucose, this bacterium able couple its oxidation reduction elemental sulfur. demonstrate furthermore M. as well MesG1 and infera are grow in syntrophic association with sulfate-reducing (SRB) acting hydrogen scavengers through interspecies transfer. Hydrogen production was higher cells co-cultured SRB than cultured alone...
Desulfovibrio species are representatives of microorganisms at the boundary between anaerobic and aerobic lifestyles, since they contain enzymatic systems required for both sulfate oxygen reduction. However, latter has been shown to be solely a protective mechanism. By implementing oxygen-driven experimental evolution vulgaris Hildenborough, we have obtained strains that evolved grow with energy derived from oxidative phosphorylation linked We show few mutations sufficient emergence this...
The combination of docking algorithms with NMR data has been developed extensively for the studies protein−ligand interactions. However, to extend this development protein−protein interactions, intermolecular NOE constraints, which are needed, more difficult access. In present work, we describe a new approach that combines an ab initio calculation and mapping interaction site using chemical shift variation analysis. cytochrome c553−ferredoxin complex is used as model numerous...