A5053623525- Neurological diseases and metabolism
- RNA regulation and disease
- Hereditary Neurological Disorders
- Cancer-related gene regulation
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Porphyrin Metabolism and Disorders
- Fibroblast Growth Factor Research
- Kruppel-like factors research
- Long-Term Effects of COVID-19
- Single-cell and spatial transcriptomics
- Cell Adhesion Molecules Research
- Drug-Induced Hepatotoxicity and Protection
- COVID-19 Clinical Research Studies
- Calpain Protease Function and Regulation
- Inflammasome and immune disorders
- Adenosine and Purinergic Signaling
- PI3K/AKT/mTOR signaling in cancer
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Metabolism and Genetic Disorders
- Peroxisome Proliferator-Activated Receptors
- Trace Elements in Health
- Systemic Sclerosis and Related Diseases
- Genomics and Chromatin Dynamics
Centre d’Immunologie de Marseille-Luminy
2009-2024
Aix-Marseille Université
2008-2024
Inserm
2008-2023
Centre National de la Recherche Scientifique
2006-2023
Institut de Neurobiologie de la Méditerranée
1998-2010
Vanin-1 is an epithelial ectoenzyme with pantetheinase activity and generating the amino-thiol cysteamine through metabolism of pantothenic acid (vitamin B(5)). Here we show that Vanin-1(-/-) mice, which lack in tissues, exhibit resistance to oxidative injury induced by whole-body gamma-irradiation or paraquat. This protection correlated reduced apoptosis inflammation reversed treating mutant animals cystamine. The better tolerance mice associated enhanced gamma-glutamylcysteine synthetase...
The membrane‐bound Vanin‐1 pantetheinase regulates tissue adaptation to stress. We investigated Vnn1 expression and its regulation in liver. is expressed by centrolobular hepatocytes. Using novel tools, we identify a soluble form of mouse human serum show the contribution cysteine catalytic activity. that liver contributes secretion PPARalpha limiting factor production. Functional PPRE sites are identified promoter. These results indicate might be reliable reporter activity
The acute phase of Crohn's disease (CD) is characterized by a large afflux polymorphonuclear leukocytes (PMNL) into the mucosa and release TNF-α. Conversion inactive TNF-α an active form requires cleavage transmembrane precursor TNF-α-converting enzyme (ADAM17), protease mainly regulated tissue inhibitor metalloproteinase 3 (TIMP3). aim present study was to investigate in vitro model PMNL transepithelial migration intestinal patients with CD expression regulation ADAM17 TIMP3 epithelial...
Vanin-1 is an epithelial pantetheinase that provides cysteamine to tissue and regulates response stress. expressed by enterocytes, its absence limits intestinal cell production of proinflammatory signals. A link between chronic active inflammation cancer illustrated in patients with ulcerative colitis, who have augmented risk developing colorectal cancer. Indeed, sustained advantageous growth conditions tumors. We examined whether cells affect tumorigenesis through vanin-1-dependent...
Inflammatory bowel diseases (IBD) are characterized during their active phase by polymorphonuclear leukocyte (PMNL) transepithelial migration. The efflux of PMNL into the mucosa is associated with production proinflammatory cytokines and release ATP from damaged necrotic cells. expression function purinergic P2X(7) receptor (P2X(7)R) in intestinal epithelial cells (IEC) its potential role "cross talk" between IEC have not been explored. aims present study were 1) to examine P2X(7)R (T84...
Vanin-1 is an epithelial pantetheinase, which regulates intestinal inflammation in mouse. We investigated whether human VNN1 levels could be associated to the susceptibility inflammatory bowel diseases (IBD) and explored participation of PPARg these processes.We studied expression colon biopsies from IBD patients. polymorphisms regulatory regions gene examined their genetic association with disease. Functional relevance single-nucleotide (SNPs) was assayed, we tested nuclear complexes...
Q fever is caused by Coxiella burnetii, a bacterium that survives in MPhi. Vanin-1 membrane-anchored pantetheinase controls tissue inflammation. Consequently, Vanin-1-deficient mice represent unique mouse model which stress-induced inflammation limited the reaction of resident cells. To investigate contribution host tissues control bacterial infection, we infected with C. burnetii. Mortality and morbidity were not affected. The lack had no effect on burnetii clearance but decreased formation...
Endothelial cell (EC) damage in systemic sclerosis (SSc) is reflected by the shedding of microparticles (MPs). The aim this study was to show that inhibiting MP release using pantethine or inactivating ATP-binding cassette transporter A1 (ABCA1) ameliorates murine SSc. First, effects on and basal oxidative nitrosative stresses ECs fibroblasts were determined vitro. then tested vivo. SSc induced BALB/c mice daily intradermal injection HOCl. Mice simultaneously treated with oral gavage. In...
Objective In the management of patients with IBD, there is a need to identify prognostic markers and druggable biological pathways improve mucosal repair probe efficacy tumour necrosis factor alpha biologics. Vnn1 pantetheinase that degrades pantetheine pantothenate (vitamin B 5 , precursor coenzyme A (CoA) biosynthesis) cysteamine. overexpressed by inflamed colonocytes. We investigated its contribution tolerance intestinal mucosa colitis-induced injury. Design performed an RNA sequencing...
Abstract Systemic sclerosis (SSc) is an autoimmune disease characterized by fibrosis of the skin and visceral organs vascular alterations. SSc pathophysiology involves systemic inflammation oxidative stress. Because vanin-1 gene (vnn1) encodes enzyme with pantetheinase activity that converts vasculoprotective pantethine into profibrotic pantothenic acid pro-oxidant cystamine, we tested this pathway in SSc. Activation vanin-1/pantetheinase was investigated wild-type BALB/c mice hypochlorous...
Like other tumors, aggressive soft tissue sarcomas (STS) use glycolysis rather than mitochondrial oxidative phosphorylation (OXPHOS) for growth. Given the importance of cofactor coenzyme A (CoA) in energy metabolism, we investigated impact Vnn1 pantetheinase-an enzyme that degrades pantetheine into pantothenate (vitamin B5, CoA biosynthetic precursor) and cysyteamine-on tumor Using two models, show Vnn1+ STS remain differentiated grow slowly, patients a detectable level VNN1 expression is...
Abstract The thymic medulla is composed of distinct epithelial cell subsets, defined in this report by the reactivity two novel antibodies, 95 and 29, raised against mouse lines. These antibodies were used to probe development wild‐type or mutant thymuses. In CD3σ‐deficient mice where thymocyte maturation arrested at CD4 − CD8 stage, few scattered + 29 cells are found. When mature thymocytes develop as CD3‐ζ/η mice, expansion organization but not cells, becomes detectable. RelB‐deficient T...