A5057698507- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Metabolomics and Mass Spectrometry Studies
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Lipid metabolism and biosynthesis
- Extracellular vesicles in disease
- Enzyme Structure and Function
- Ion-surface interactions and analysis
- Sphingolipid Metabolism and Signaling
- Glycosylation and Glycoproteins Research
- Protein Structure and Dynamics
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Advanced biosensing and bioanalysis techniques
- X-ray Diffraction in Crystallography
- Cancer, Lipids, and Metabolism
- Crystallization and Solubility Studies
- Endoplasmic Reticulum Stress and Disease
- Molecular Biology Techniques and Applications
- Eicosanoids and Hypertension Pharmacology
- MicroRNA in disease regulation
- Hemoglobin structure and function
- Monoclonal and Polyclonal Antibodies Research
The University of Melbourne
2016-2025
Michigan State University
2007-2024
Florey Institute of Neuroscience and Mental Health
2023
Prince of Wales Hospital
2012-2015
Liverpool Hospital
1998-2015
St George Hospital
2012-2015
St Vincent’s Private Hospital Sydney
2014-2015
Michigan United
2012-2014
La Trobe University
2011
Nepean Hospital
2010
Extracellular vesicles (EVs) are membraneous released by a variety of cells into their microenvironment. Recent studies have elucidated the role EVs in intercellular communication, pathogenesis, drug, vaccine and gene-vector delivery, as possible reservoirs biomarkers. These findings generated immense interest, along with an exponential increase molecular data pertaining to EVs. Here, we describe Vesiclepedia, manually curated compendium (lipid, RNA, protein) identified different classes...
Exosomes are membraneous nanovesicles of endocytic origin released by most cell types from diverse organisms; they play a critical role in cell–cell communication. ExoCarta (http://www.exocarta.org) is manually curated database exosomal proteins, RNA and lipids. The catalogs information both published unpublished studies. mode purification characterization, the biophysical molecular properties listed aiding biomedical scientists assessing quality preparation corresponding data obtained....
Lipid dyshomeostasis is associated with the most common form of dementia, Alzheimer's disease (AD). Substantial progress has been made in identifying positron emission tomography and cerebrospinal fluid biomarkers for AD, but they have limited use as front-line diagnostic tools. Extracellular vesicles (EVs) are released by all cells contain a subset their parental cell composition, including lipids. EVs from brain into periphery, providing potential source tissue specific lipid biomarkers....
The dynamic nature of bacterial lipid diversity and membrane properties in response to stress conditions is discussed. This emerging area has implications for a range cellular processes drug design selective cell targeting.
Apical membrane antigen-1 (AMA-1) of Plasmodium falciparum is one the leading asexual blood stage antigens being considered for inclusion in a malaria vaccine. The ability this molecule to induce protective immune response has been shown be dependent upon conformation stabilized by disulfide bonds. In study we have utilized reversed-phase high performance liquid chromatography dithiothreitol-reduced and nonreduced tryptic digests chabaudi AMA-1 secreted from baculovirus-infected insect...
A database of 5500 unique peptide tandem mass spectra acquired in an ion trap spectrometer was assembled for peptides derived from proteins digested with trypsin. Peptides were identified initially their by the SEQUEST algorithm and subsequently validated manually. Two different statistical methods used to identify sequence-dependent fragmentation patterns that could be improve models incorporated into current sequencing search algorithms. The currently accepted "mobile proton" model...
When murine interleukin-6 is overexpressed in Escherichia coli, a small population of molecules exhibits novel C-terminal modification. Peptide mapping, electrospray ionization-mass spectrometry, and automated N- sequencing identified peptide ("tag" peptide), -Ala-Ala-Asn-Asp-Glu-Asn-Tyr-Ala-Leu-Ala-Ala-COOH, encoded by metabolically stable RNA E. coli (10Sa RNA) attached to truncated C termini the recombinant protein. A mutant strain which chromosomal 10Sa gene (ssrA) disrupted does not...
The proteomic definition of plasma membrane proteins is an important initial step in searching for novel tumor marker expressed during the different stages cancer progression. However, due to charge heterogeneity and poor solubility membrane-associated this subsection cell's proteome often refractory two-dimensional electrophoresis (2-DE), current paradigm technology studying protein expression profiles. Here, we describe a non-2-DE method identifying proteins. Proteins from enriched...
When proteostasis becomes unbalanced, unfolded proteins can accumulate and aggregate. Here we report that the dye, tetraphenylethene maleimide (TPE-MI) be used to measure cellular protein load. TPE-MI fluorescence is activated upon labelling free cysteine thiols, normally buried in core of globular are exposed unfolding. Crucially does not become fluorescent when conjugated soluble glutathione. We find enhanced reaction with proteomes under conditions promoting accumulation proteins....
The development of strategies directed toward comprehensive analysis the phosphoproteome have undoubtedly been facilitated by recent advances in application ion trap tandem mass spectrometry-based techniques for routine phosphopeptide identification. However, when multiple potential sites phosphorylation exist within a phosphorylated peptide sequence, unambiguous characterization site remains significant challenge. Here, gas-phase fragmentation reactions series 33 synthetic phospho-serine,...
Objective To evaluate preoperative real-time dynamic transvaginal sonography (TVS) in the prediction of pouch Douglas (POD) obliteration women undergoing laparoscopy for suspected endometriosis. METHODS This was a multicenter prospective observational study undertaken from January 2009 to November 2011. All with symptoms suggestive endometriosis who were scheduled underwent detailed TVS, particular ascertain whether POD obliterated. assessed using TVS technique called ‘sliding sign’....
A "shotgun" lipidomics strategy consisting of sequential functional group selective chemical modification reactions coupled with high-resolution/accurate mass spectrometry and "targeted" tandem (MS/MS) analysis has been developed applied toward the comprehensive identification, characterization quantitative changes in relative abundances >600 individual glycerophospholipid, glycerolipid, sphingolipid sterol lipids between a primary colorectal cancer (CRC) cell line, SW480, its isogenic lymph...
Competing models exist in the literature for relationship between mutant Huntingtin exon 1 (Httex1) inclusion formation and toxicity. In one, inclusions are adaptive by sequestering proteotoxicity of soluble Httex1. other, compromise cellular activity as a result proteome co-aggregation. Using biosensor Httex1 conformation mammalian cell models, we discovered mechanism that reconciles these competing models. Newly formed were composed disordered ribonucleoproteins. As matured, reconfigured...
Sphingolipids serve not only as components of cellular membranes but also bioactive mediators numerous functions. As the biological activities these lipids are dependent on their structures, and due to limitations conventional ion activation methods employed during tandem mass spectrometry (MS/MS), there is a recognized need for development improved structure-specific comprehensive identification characterization. Here, positive-ionization mode 193 nm ultraviolet photodissociation...
ConspectusLipids play critical structural and functional roles in the regulation of cellular homeostasis, it is increasingly recognized that disruption lipid metabolism or signaling both associated with onset progression certain metabolically linked diseases. As a result, field lipidomics has emerged to comprehensively identify structurally characterize diverse range species within sample interest quantitatively monitor their abundances under different physiological pathological conditions....