A5047358063- Cellular transport and secretion
- Antibiotic Resistance in Bacteria
- Mitochondrial Function and Pathology
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Lipid Membrane Structure and Behavior
- Cellular Mechanics and Interactions
- Sirtuins and Resveratrol in Medicine
- Gastric Cancer Management and Outcomes
- Pneumonia and Respiratory Infections
- Autophagy in Disease and Therapy
- Bacterial Identification and Susceptibility Testing
- Lung Cancer Treatments and Mutations
- Genetic factors in colorectal cancer
- Cancer Immunotherapy and Biomarkers
- Viral Infections and Vectors
- Genomics and Phylogenetic Studies
- Cell Adhesion Molecules Research
- Prostate Cancer Treatment and Research
- Radiopharmaceutical Chemistry and Applications
- Antibiotics Pharmacokinetics and Efficacy
- Helicobacter pylori-related gastroenterology studies
- Hepatitis B Virus Studies
- Rice Cultivation and Yield Improvement
- Viral gastroenteritis research and epidemiology
Jiangxi Normal University
2021-2025
First Affiliated Hospital of Nanchang University
2023-2024
Nanchang University
2014-2024
The First Affiliated Hospital, Sun Yat-sen University
2024
Sun Yat-sen University
2023-2024
Case Western Reserve University
2021-2023
First Affiliated Hospital of Guangzhou Medical University
2020-2023
State Key Laboratory of Respiratory Disease
2020-2023
Guangzhou Medical University
2020-2023
Second People’s Hospital of Huai’an
2007-2023
The mammalian target of rapamycin (MTOR) protein kinase complex is a key component pathway that regulates cell growth and proliferation in response to energy levels, hypoxia, nutrients insulin. Inhibition MTORC1 strongly induces autophagy by regulating the activity ULK required for formation autophagosomes. However, participation expression genes has not been characterized. Here we show nuclear localization transcription factor EB (TFEB), member bHLH leucine-zipper family factors drives...
The healthy vascular endothelium, which forms the barrier between blood and surrounding tissues, is known to efficiently respond stress signals like hypoxia inflammation by adaptation of cellular physiology secretion (soluble) growth factors cytokines. Exosomes are potent mediators intercellular communication. Their content consists RNA proteins from cell origin, thus depends on condition these cells at time exosome biogenesis. It has been suggested that exosomes protect their target through...
Control of the establishment cell polarity is an essential function in tissue morphogenesis and renewal that depends on spatial cues provided by extracellular environment. The molecular role cell-cell or cell-extracellular matrix (ECM) contacts has been well characterized. It hypothesized geometry adhesive microenvironment was directing surface polarization internal organization. To define how environment affects polarity, we analyzed organization individual cells plated defined...
Cells display a large variety of shapes when plated in classical culture conditions despite their belonging to common cell type. These are transitory, since cells permanently disassemble and reassemble cytoskeleton while moving. Adhesive micropatterns commonly used confine shape within given geometry. In addition the micropattern can be designed so as impose spread upon adhesive nonadhesive areas. Modulation pattern geometry allows analysis mechanisms governing determination response...
ABSTRACT Carbapenem-resistant Enterobacteriaceae (CRE) have spread globally and represent a serious growing threat to public health. Rapid methods for tracking plasmids carrying carbapenemase genes could greatly benefit infection control efforts. Here, we demonstrate that real-time, direct of single plasmid in bacterial strain responsible an outbreak is possible using commercial matrix-assisted laser desorption ionization–time flight mass spectrometry (MALDI-TOF MS) system. In this case,...
Background/Aims: LncRNAs have been reported to be vital regulators of the progression osteosarcoma, although underlying mechanisms are not completely understood. Methods: The levels MALAT1 and miR-129-5p expression were measured using qRT-PCR. Cell growth was determined CCK-8 colony formation assays. migration invasion detected wound healing Transwell assays, respectively. Tumor with a xenograft model. Results: significantly up-regulated in osteosarcoma tissues compared adjacent non-tumor...
As nutrient-sensing nuclear/cytosolic acetylation mediates cellular autophagy, we investigated whether mitochondrial modulates autophagy. Knockdown of GCN5L1, a component the acetyltransferase machinery, diminished protein and augmented enrichment autophagy mediators. This program was disrupted by Sirt3 knockdown. Chronic GCN5L1 depletion increased turnover reduced content/mass. In parallel, mitochondria showed blunted respiration enhanced ‘stress-resilience’. Genetic disruption mediators...
Drugs that activate protein kinase G could mimic the bone-building effects of mechanical stimulation.
Although inhibition of cyclic nucleotide phosphodiesterase type 3 (PDE3) has been reported to protect rodent heart against ischemia/reperfusion (I/R) injury, neither the specific PDE3 isoform involved nor underlying mechanisms have identified. Targeted disruption subfamily B (PDE3B), but not A (PDE3A), protected mouse from I/R injury in vivo and vitro, with reduced infarct size improved cardiac function. The cardioprotective effect PDE3B(-/-) was reversed by blocking cAMP-dependent PKA...
Abstract The mitochondrial enriched GCN5-like 1 (GCN5L1) protein has been shown to modulate acetylation, content and retrograde signaling. Here we show that hepatic GCN5L1 ablation reduces fasting glucose levels blunts gluconeogenesis without affecting systemic tolerance. PEPCK G6Pase transcript are downregulated in hepatocytes from liver specific knockout mice their upstream regulator, FoxO1 decreased via proteasome-dependent degradation reactive oxygen species mediated ERK-1/2...
Organic acidemias such as methylmalonic acidemia (MMA) are a group of inborn errors metabolism that typically arise from defects in the catabolism amino and fatty acids. Accretion acyl-CoA species is postulated to underlie disease pathophysiology, but mechanism(s) remain unknown. Here, we surveyed hepatic explants patients with MMA unaffected donors, parallel samples various mouse models methylmalonyl-CoA mutase deficiency. We found widespread posttranslational modification,...
Rationale: Mice lacking cyclophilin D (CypD −/− ), a mitochondrial chaperone protein, have altered cardiac metabolism. As acetylation has been shown to regulate metabolism, we tested whether changes in protein might play role these metabolic CypD hearts. Objective: Our aim was test the hypothesis that loss of alters acetylome. Methods and Results: To identify lysine-acetylated proteins map sites after ablation CypD, subjected tryptic digests isolated mitochondria from wild-type mice...