A5048998029- Extracellular vesicles in disease
- Acoustic Wave Resonator Technologies
- Molecular Junctions and Nanostructures
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- Mechanical and Optical Resonators
- Advanced Biosensing Techniques and Applications
- Analytical Chemistry and Sensors
- Microfluidic and Capillary Electrophoresis Applications
- Nanofabrication and Lithography Techniques
- Biosensors and Analytical Detection
- Force Microscopy Techniques and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanopore and Nanochannel Transport Studies
- Advanced MEMS and NEMS Technologies
- MicroRNA in disease regulation
- Genetics, Bioinformatics, and Biomedical Research
- Pesticide and Herbicide Environmental Studies
- DNA and Nucleic Acid Chemistry
- Monoclonal and Polyclonal Antibodies Research
- Biotechnology and Related Fields
- Characterization and Applications of Magnetic Nanoparticles
- Pesticide Exposure and Toxicity
- Photonic and Optical Devices
- Blood properties and coagulation
Franche-Comté Électronique Mécanique Thermique et Optique - Sciences et Technologies
2016-2025
Centre National de la Recherche Scientifique
2012-2025
Université de franche-comté
2008-2024
Université Bourgogne Franche-Comté
2016-2022
Université de Bourgogne
2016-2022
École Nationale Supérieure de Mécanique et des Microtechniques
2016-2020
Université de technologie de belfort-montbéliard
2016-2020
Université de Technologie de Compiègne
2016
TU Dresden
2009
ABSTRACT The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions extracellular vesicles (EVs), collective term covering various subtypes cell‐released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, many other names. However, specific issues arise when working with these entities, whose size amount often make them difficult to obtain as relatively...
ABSTRACT Noroviruses (NoVs) are one of the leading causes gastroenteritis in children and adults. For last 2 decades, genogroup II genotype 4 (GII.4) NoVs have been circulating worldwide. GII.4 can be divided into variants, since 2002 they circulated population before being replaced every or 3 years, which raises questions about role their histo-blood group antigen (HBGA) ligands evolution. To shed light on these questions, we performed an analysis interaction between representative variants...
Recent advancements in the clinical EV field highlight their potential as biomarkers for diverse diseases and therapeutic applications. This study provides an in-depth characterization of 10K EVs from human microvascular endothelial cells (HMEC-1) exposed to benzo-a-pyrene (B[a]P), a polycyclic aromatic hydrocarbon found food smoke. Given EVs’ complexity, with numerous surface cargo proteins, phenotyping remains challenging. Here, we introduce multiplex biosensor profiling distinct cellular...
Recent advances in the clinical extracellular vesicles (EVs) field highlight their potential as biomarkers for diverse diseases and therapeutic applications. This study provides an in-depth characterization of 10k EVs from human microvascular endothelial cells (HMEC-1) exposed to benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon found food smoke. Given EVs' complexity, with numerous surface cargo proteins, phenotyping remains challenging. Here, we introduce multiplex biosensor,...
We report on the formation kinetics of mixed self-assembled monolayers (SAMs) comprising 16-mercaptohexadecanoic acid (MHDA) and 11-mercapto-1-undecanol (MUDO) thiols GaAs(100) substrates. These compounds were selected for their potential in constructing highly selective efficient architectures biosensing applications. The molecular composition quality one-compound SAMs determined by Fourier transform infrared absorption spectroscopy measurements. enhanced-quality was investigated as a...
In this paper we describe a new surface plasmon resonance (SPR) biosensor dedicated to potential estrogenic compounds prescreening, by developing an estrogen receptor (ER) specific DNA chip. Through the covalent binding of strain wearing response element (ERE) activated 6-mercapto-1-hexadecanoic acid and 11-mercapto-1-undecanol self-assembled monolayer on gold surface, SPR allows detect specifically, quickly, without any labeling ER in presence estrogen. parallel, investigated interaction...
This article details the surface-enhanced Raman scattering (SERS) performance of plasmonic substrates fabricated by a physical metal evaporation technique that uses no precursor or intermediate coating. We outline cost-effective nanofabrication protocol common laboratory equipment to produce homogeneously covered crystalline nanoparticle substrates. Our fabrication yields homogeneous SERS response over whole surface. The platform is tested with methylene blue diluted at various...
Abstract N-cadherin is a transmembrane glycoprotein expressed by mesenchymal origin cells and located at the adherens junctions. It regulates also cell motility contributes to signaling. In previous studies, we identified that its anomalous expression in bladder carcinoma was tumor progression marker. A pharmacological approach inhibit or block function could be relevant prevent disease metastasis development. The morphological exploration of T24 invasive cancer atomic force microscopy (AFM)...
GaAs crystal presents some interesting perspectives for resonant biosensors due to its piezoelectric and good mechanical properties the opportunity bio-functionalize surface. Moreover, can be micromachined by wet etching in several solutions, which constitutes a batch low-cost process of fabrication. The lateral field excitation (LFE) is used generate bulk acoustic waves. main advantage LFE possibility measure liquid media, moreover reduced aging increased frequency stability are also...
Among the enduring challenges in nanoscience, subsurface characterization of living cells holds major stakes. Developments nanometrology for soft matter thriving on sensitivity and high resolution benefits atomic force microscopy have enabled detection structures at nanoscale. However, measurements liquid environments remain complex, particular domain. Here we introduce liquid-mode synthesizing (l-MSAFM) to study both inner chemically induced intracellular impairments cells. Specifically,...
We report Gold/Silica biochips for low cost biosensor devices. Firstly, the study of biochemical interactions on silica by means Surface Plasmon Resonance (SPR) is presented. Secondly, are employed to reduce strong quenching that occurs when a fluorophore close gold surface. Furthermore, control Silica-like thickness allows optimizing distance between metallic surface and in order enhance fluorescent signal. These results represent first steps towards highly sensitive, specific biosensors...
Widely used in microelectronics and optoelectronics; Gallium Arsenide (GaAs) is a III-V crystal with several interesting properties for microsystem biosensor applications. Among these; its piezoelectric the ability to directly biofunctionalize bare surface, offer an opportunity combine highly sensitive transducer specific bio-interface; which are two essential parts of biosensor. To optimize biorecognition part; it necessary control protein coverage binding affinity layer on GaAs surface. In...
The work described here concerns the fabrication of cost-effective biosensors that permit to amplify a fluorescence signal without complex nano-structuration surface. idea is put profit natural pseudo nano-structuring observed when depositing metallic layers by various micro-fabrication techniques. This new architecture consists glass substrate. A gold film deposited on top it and silica layer onto gold. dye (Cy5) then absorbed surface intensity measured. depends distance between metal. It...
Wet chemical processes were investigated to remove alkanethiol self-assembled monolayers (SAMs) and regenerate GaAs (001) samples studied in the context of development reusable devices for biosensing applications. The authors focused on 16-mercaptohexadecanoic acid (MHDA) SAMs that are commonly used produce an interface between antibodies or others proteins metallic semiconductor substrates. As determined by Fourier transform infrared absorption spectroscopy, among solutions HCl, H2O2,...
A regenerable bulk acoustic wave (BAW) biosensor is developed for the rapid, label-free and selective detection of Escherichia coli in liquid media. The geometry consists a GaAs membrane coated with thin film piezoelectric ZnO on its top surface. pair electrodes deposited allows generation BAWs by lateral field excitation. back surface functionalized alkanethiol self-assembled monolayers antibodies against E. coli. antibody immobilization was investigated as function concentration...
Extracellular vesicles (EVs) are membrane-derived, tiny produced by all cells that range from 50 to several hundreds of nanometers in diameter and used as a means intercellular communication. They emerging promising diagnostic therapeutic tools for variety diseases. There two main biogenesis processes produce EVs with differences size, composition, content. Due their high complexity cell origin, characterization requires combination analytical techniques. This project involves the...