A5058778314- Extracellular vesicles in disease
- Cardiovascular Disease and Adiposity
- Blood properties and coagulation
- MicroRNA in disease regulation
- Atherosclerosis and Cardiovascular Diseases
- Immune Cell Function and Interaction
- Biosensors and Analytical Detection
- Signaling Pathways in Disease
- Circular RNAs in diseases
- Inhalation and Respiratory Drug Delivery
- Advanced Proteomics Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Adenosine and Purinergic Signaling
- Nitric Oxide and Endothelin Effects
- RNA Research and Splicing
- Horticultural and Viticultural Research
- Cardiac Ischemia and Reperfusion
Lund University
2016-2025
ABSTRACT The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions extracellular vesicles (EVs), collective term covering various subtypes cell‐released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, many other names. However, specific issues arise when working with these entities, whose size amount often make them difficult to obtain as relatively...
Extracellular vesicles (ECVs), including microparticles and exosomes, are submicrometer membrane released by diverse cell types upon activation or stress. Circulating ECVs potential reservoirs of disease biomarkers, the complexity these is significantly lower compared to their source, blood plasma, which makes ECV-based biomarker studies more promising. Proteomic profiling important not only discover new diagnostic prognostic markers but also understand roles in biological function. In...
We investigated the mRNA expression of G protein-coupled receptors (GPCRs) in cells from four human vascular beds: umbilical vein (HUVEC), microvasculature (MVEC), aorta (HAEC), and coronary artery (CAEC). Our study revealed that orphan receptor ELTD1 (ADGRL4) was most abundantly expressed GPCR all EC types. When recombinantly U87 cells, activated canonical pathways, particularly Gq pathway. Conditioned medium also HEK293 supporting existence an autocrine ELTD1-activating factor. Using...
Endothelial cells release ATP in response to fluid shear stress, which activates purinergic (P2) receptor-mediated signaling molecules including endothelial nitric oxide (eNOS), a regulator of vascular tone. While P2 the vasculature is well studied, role P2Y2 receptors stress-associated cell alignment, cytoskeletal alterations, and wound repair remains ill defined. To address these aspects, human umbilical vein (HUVEC) monolayers were cultured on gelatin-coated dishes subjected stress 1 Pa....
Improved reproducibility in seed particle mediated acoustic trapping of submicron particles enables clinical biomarker studies extracellular vesicles.
Diet rich in bilberries is considered cardioprotective, but the mechanisms of action are poorly understood. Cardiovascular disease characterized by increased proatherogenic status and high levels circulating microvesicles (MVs). In an open-label study patients with myocardial infarction receive 8 week dietary supplementation bilberry extract (BE). The effect BE on patient MV its influence endothelial vesiculation vitro investigated.MVs captured acoustic trapping platelet-derived MVs (PMVs),...
We investigated how microvesicles (MVs) from patients with cardiovascular diseases are related to coronary flow reserve (CFR), a clinical parameter reflecting blood in the heart. Our results show negative relationship between CFR and levels of platelet endothelial MVs. The pattern MV-enriched biomarkers differs high low CFR. findings suggest potential value MVs as reduced proatherogenic status, additional
Abstract Background Endothelial and microvascular dysfunction are pivotal causes of major adverse cardiac events predicted by coronary flow reserve (CFR). Extracellular Vesicles (EVs) have been studied extensively in the pathophysiology artery disease. However, little is known on impact non-coding RNA content EVs with respect to CFR. Methods We carried out a study among 120 patients divided high-CFR low-CFR profile miRNA circulating EVs. Results A multiplex array profiling revealed...
Objective Endothelial dysfunction, inflammation and platelet activation are conditions that predispose for development of cardiovascular disease. These also previously shown to trigger release microparticles (MPs). As isolation MPs from plasma is time consuming requires large sample volumes, we decided develop an alternative method based on acoustic trapping. Acoustic trapping a non‐contact where sound waves used retain particles in microfluidic channel ( Figure 1 ). Exciting capillary with...