Dolores Di Vizio
A5059046046- Extracellular vesicles in disease
- Prostate Cancer Treatment and Research
- MicroRNA in disease regulation
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Caveolin-1 and cellular processes
- Cell Adhesion Molecules Research
- Nuclear Structure and Function
- RNA Research and Splicing
- Estrogen and related hormone effects
- Steroid Chemistry and Biochemistry
- Glutathione Transferases and Polymorphisms
- Ubiquitin and proteasome pathways
- Cholesterol and Lipid Metabolism
- Circular RNAs in diseases
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Gastrointestinal Tumor Research and Treatment
- Nanoplatforms for cancer theranostics
- Cancer-related Molecular Pathways
- Peptidase Inhibition and Analysis
- DNA Repair Mechanisms
- Tissue Engineering and Regenerative Medicine
- Sarcoma Diagnosis and Treatment
- Cancer Research and Treatments
Cedars-Sinai Medical Center
2016-2025
Boston Children's Hospital
2011-2025
Los Angeles Medical Center
2023
University of California, Los Angeles
2016-2020
Harvard University
2009-2019
Samueli Institute
2014-2019
Jagiellonian University
2014
University Medical Center
2014
Pohang University of Science and Technology
2014
AGH University of Krakow
2014
ABSTRACT The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions extracellular vesicles (EVs), collective term covering various subtypes cell‐released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, many other names. However, specific issues arise when working with these entities, whose size amount often make them difficult to obtain as relatively...
Secreted membrane‐enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology medicine. Importantly, it is currently technically challenging to obtain totally pure fraction free from non‐vesicular components for functional studies, therefore there need establish guidelines analyses of these reporting studies on biology....
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of cell origin and change functions phenotypes other cells. These features indicate strong biomarker therapeutic potential have generated broad interest, as evidenced by steady year-on-year increase in numbers scientific publications about EVs. Important advances been made EV metrology understanding applying biology. However, hurdles remain to realising EVs domains ranging from basic biology clinical...
Caveolin-1 is the principal structural protein of caveolae membranes in fibroblasts and endothelia. Recently, we have shown that human CAV-1 gene localized to a suspected tumor suppressor locus, mutations Cav-1 been implicated cancer. Here, created caveolin-1 null (CAV-1 −/−) mouse model, using standard homologous recombination techniques, assess role biogenesis, endocytosis, cell proliferation, endothelial nitric-oxide synthase (eNOS) signaling. Surprisingly, mice are viable. We show these...
Extracellular vesicles (EVs) represent an important mode of intercellular communication. Research in this field has grown rapidly the last few years, and there is a plethora techniques for isolation characterization EVs, many which are poorly standardized. EVs heterogeneous size, origin molecular constituents, with considerable overlap size phenotype between different populations EVs. Little known about current practices isolation, purification We report here first large, detailed survey...
The pyruvate kinase M2 isoform (PKM2) is expressed in cancer and plays a role regulating anabolic metabolism. To determine whether PKM2 required for tumor formation or growth, we generated mice with conditional allele that abolishes expression without disrupting PKM1 expression. deletion accelerated mammary Brca1-loss-driven model of breast cancer. null tumors displayed heterogeneous expression, found nonproliferating cells no detectable proliferating cells. This suggests not necessary cell...
The goal of this study was to understand if exosomes derived from high-metastatic cells may influence the behavior less aggressive cancer and properties endothelium. We found that metastatic colon are able transfer their amoeboid phenotype isogenic primary through exosomes, morphological transition is associated with acquisition a more behavior. Moreover, line (SW620Exos) exhibited higher ability cause endothelial hyperpermeability than non (SW480Exos). SWATH-based quantitative proteomic...
Oncosomes have recently been described as membrane-derived microvesicles secreted by cancer cells, which transfer oncogenic signals and protein complexes across cell boundaries. Here, we show the rapid formation secretion of oncosomes from DU145 LNCaP human prostate cells. Oncosome was stimulated epidermal growth factor receptor activation also overexpression membrane-targeted Akt1. Microvesicles shed cells contained numerous signal transduction proteins were capable activating...
Oncosomes are tumor-derived microvesicles that transmit signaling complexes between cell and tissue compartments. Herein, we show amoeboid tumor cells export large (1- to 10-μm diameter) vesicles, derived from bulky cellular protrusions, contain metalloproteinases, RNA, caveolin-1, the GTPase ADP-ribosylation factor 6, biologically active toward cells, endothelial fibroblasts. We describe methods by which oncosomes can be selectively sorted flow cytometry analyzed independently of vesicles...
Abstract Motivation: Extracellular vesicles (EVs) are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of vesicular nomenclatures and components, online searches for EV-related publications components currently challenging. Results: We present an improved version EVpedia, a public database EVs research. This community web portal contains identification orthologous bioinformatic tools personalized function. EVpedia includes 6879 publications, 172...
// Valentina R. Minciacchi 1 , Sungyong You Cristiana Spinelli Samantha Morley 2 Mandana Zandian Paul-Joseph Aspuria 3 Lorenzo Cavallini 1,4 Chiara Ciardiello 1,5 Mariana Reis Sobreiro Matteo Morello Geetanjali Kharmate 6 Su Chul Jang 7 Dae-Kyum Kim Elham Hosseini-Beheshti Emma Tomlinson Guns Martin Gleave Yong Song Gho Suresh Mathivanan 8 Wei Yang Michael Freeman 1,2 and Dolores Di Vizio Division of Cancer Biology Therapeutics, Departments Surgery, Biomedical Sciences Pathology Laboratory...
ABSTRACT Bioinformatics tools are imperative for the in depth analysis of heterogeneous high‐throughput data. Most software developed by specific laboratories or groups companies wherein they designed to perform required group. However, such may fail capture “what community needs a tool”. Here, we describe novel community‐driven approach build comprehensive functional enrichment tool. Using existing FunRich tool as template, invited researchers request additional features and/or changes....
Cancer-derived extracellular vesicles (EVs) are membrane-enclosed structures of highly variable size. EVs contain a myriad substances (proteins, lipid, RNA, DNA) that provide reservoir circulating molecules, thus offering good source biomarkers. We demonstrate here large (L-EV) (large oncosomes) isolated from prostate cancer (PCa) cells and patient plasma an EV population is enriched in chromosomal DNA, including fragments up to 2 million base pair long. While L-EVs small (S-EV) (exosomes)...
Protein S-acylation (palmitoylation), a reversible post-translational modification, is critically involved in regulating protein subcellular localization, activity, stability, and multimeric complex assembly. However, proteome scale characterization of has lagged far behind that phosphorylation, global analysis the localization S-acylated proteins within different membrane domains not been reported. Here we describe novel proteomics approach, designated palmitoyl identification site...
Modification by acetylation occurs at -amino lysine residues of histones and transcription factors.Unlike phosphorylation, a direct link between factor cellular growth or apoptosis has not been established.We show that the nuclear androgen receptor (AR), DNA-binding transcriptional regulator, is acetylated in vivo.The AR induced ligand dihydrotestosterone histone deacetylase (HDAC) inhibitors living cells.Direct augmented p300 binding vitro.Constructs mimicking neutral polar substitution (AR...
Evaluation of kinetic distribution and behaviors nanoparticles in vivo provides crucial clues into their roles living organisms. Extracellular vesicles are evolutionary conserved nanoparticles, known to play important biological functions intercellular, inter-species, inter-kingdom communication. In this study, the first analysis biodistribution outer membrane (OMVs)—bacterial extracellular vesicles—with immune-modulatory is performed. OMVs, injected intraperitoneally, spread whole mouse...