A5064747640- Extracellular vesicles in disease
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Food Allergy and Anaphylaxis Research
- Cancer Cells and Metastasis
- Acute Kidney Injury Research
- Cell death mechanisms and regulation
- Chronic Kidney Disease and Diabetes
- Macrophage Migration Inhibitory Factor
- Dialysis and Renal Disease Management
- Bone Metabolism and Diseases
- Asthma and respiratory diseases
- Cardiovascular Disease and Adiposity
- Immunotherapy and Immune Responses
- Phagocytosis and Immune Regulation
- Endoplasmic Reticulum Stress and Disease
- Vitamin C and Antioxidants Research
- NF-κB Signaling Pathways
- Occupational and environmental lung diseases
- Allergic Rhinitis and Sensitization
- Pancreatitis Pathology and Treatment
- Nuclear Receptors and Signaling
- Mast cells and histamine
- Renal and related cancers
- Lymphatic System and Diseases
Universidad Alfonso X el Sabio
2022-2024
Hospital Clínico San Carlos
2024
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos
2024
Cornell University
2015-2023
Lehman College
2023
City University of New York
2023
Weill Cornell Medicine
2018-2023
Hospital Universitario Fundación Jiménez Díaz
2008-2022
Children's Cancer and Blood Foundation
2015-2022
Universidad Autónoma de Madrid
2008-2015
ABSTRACT Bioinformatics tools are imperative for the in depth analysis of heterogeneous high‐throughput data. Most software developed by specific laboratories or groups companies wherein they designed to perform required group. However, such may fail capture “what community needs a tool”. Here, we describe novel community‐driven approach build comprehensive functional enrichment tool. Using existing FunRich tool as template, invited researchers request additional features and/or changes....
Galectin-3 (Gal-3) participates in different mechanisms involved atherothrombosis, such as inflammation, proliferation, or macrophage chemotaxis. Thus, there have been committed intensive efforts to elucidate the function of Gal-3 cardiovascular (CV) diseases. The role a circulating biomarker has demonstrated patients with heart failure, but its importance atherothrombosis is still unknown.Because monocyte-to-macrophage transition, we used fresh isolated monocytes and vitro model...
Urinary extracellular vesicles provide a novel source for valuable biomarkers kidney and urogenital diseases: Current isolation protocols include laborious, sequential centrifugation steps which hampers their widespread research clinical use. Furthermore, large individual urine sample volumes or sizable target cohorts are to be processed (e.g. biobanking), the storage capacity is an additional problem. Thus, alternative methods necessary overcome such limitations. We have developed practical...
The hypothesis that microvesicle-mediated miRNA transfer converts noncancer stem cells into cancer (CSC) leading to therapy resistance remains poorly investigated. Here we provide direct evidence supporting this hypothesis, by demonstrating how microvesicles derived from cancer-associated fibroblasts (CAF) miR-221 promote hormonal (HTR) in models of luminal breast cancer. We determined CAF-derived horizontally transferred tumor and, combination with hormone therapy, activated an ER
Liquid biopsies from cancer patients have the potential to improve diagnosis and prognosis. The assessment of surrogate markers tumor progression in circulating extracellular vesicles could be a powerful non-invasive approach this setting. We characterized purified lymphatic drainage also known as exudative seroma (ES) stage III melanoma obtained after lymphadenectomy. Proteomic analysis showed that seroma-derived exosomes are enriched proteins resembling progression. In addition, we found...
Apoptotic cell death contributes to diabetic nephropathy (DN), but its role is not well understood. The tubulointerstitium from DN biopsy specimens was microdissected, and expression profiles of genes related apoptosis were analyzed. A total 112 (25%) 455 death–related found be significantly differentially regulated. Among those that showed the greatest changes in regulation two receptors, <i>OPG</i> (the gene encoding osteoprotegerin) <i>Fas</i>, ligand <i>TRAIL.</i> Glomerular proximal...
The aim of this paper is to study the myocardial damage secondary long-term streptozotocin-induced type 1 diabetes mellitus (DM1). Normotensive and spontaneously hypertensive rats (SHR) received either streptozotocin injections or vehicle. After 22 6 wk, DM1, SHR, DM1/SHR, control were killed, left ventricles studied by histology, quantitative PCR, Western blot, ELISA, electromobility shift assay. Cardiomyocyte cultures also performed. expression profibrotic factors, transforming growth...
TWEAK (tumor necrosis factor-like weak inducer of apoptosis) is a TNF superfamily cytokine that activates the fibroblast growth factor-inducible 14 (Fn14) receptor. Transcriptional analysis experimental kidney tubulointerstitial inflammation showed correlation between an upregulation mRNA for transmembrane chemokine CXCL16, T-cell chemoattractant, and Fn14 activation. Exogenous increased mouse CXCL16 expression T-lymphocyte infiltration in vivo, processes inhibited by NF-κB inhibitor...
Urinary exosomes have been proposed as potential diagnostic tools. TNF superfamily cytokines and receptors may be present in are expressed by proximal tubular cells. We now studied the expression of selected proteins exosome-like vesicles from cultured human cells urine identified additional these LC-MS/MS proteomics. Human constitutively released that did not contain TRAIL or TWEAK. However, contained osteoprotegerin (OPG), a receptor protein, assessed Western blot, ELISA reaction...
Abstract A range of cell types, including embryonic stem cells, neurons and astrocytes have been shown to release extracellular vesicles (EVs) containing molecular cargo. Across EVs facilitate transfer mRNA, microRNA proteins between cells. Here we describe the kinetics content from mouse retinal progenitor cells (mRPCs). Interestingly, mRPC derived contain miRNA associated with multipotency development. Transcripts enclosed in EVs, include transcription factors Pax6, Hes1, Sox2, a mitotic...
Genetic or acquired defects of the lymphatic vasculature often result in disfiguring, disabling, and, occasionally, life-threatening clinical consequences. Advanced forms lymphedema are readily diagnosed clinically, but more subtle presentations require invasive imaging other technologies for a conclusive diagnosis. On hand, lipedema, chronic microvascular disease with pathological accumulation subcutaneous adipose tissue, is misdiagnosed as obesity lymphedema; currently there no biomarkers...